Suppl. Fig. 1

A B

HP

DE

PA

NC

1

Su

it02

8

Su

it00

7

Ca

pa

nI

Pa

Tu

89

88

t

Mo

no

Ma

c 6

TGF

Actin

A

Suppl. Fig. 2

empt

y ve

ctor

CU

X1

CUX1

β-Actin

ED

CUX1

β-Actin

un

tre

ate

d

Ctr

l siR

NA

CU

X1

siR

NA

#1

CU

X1

siR

NA

#2

CU

X1

siR

NA

#3

B CCXCL10 CCL5

NFkB p65

0 2 4 24 0 2 4 24 LV CUX1

CUX1

STAT1

β-Actin

STAT3

STAT6

NFkB p50

Phospho Stat3 (Ser727)

phosphoNFkBp65 (Ser536)

A

Suppl. Fig. 3

TNF (incubation time in hours)

β-Actin

STAT1

STAT3

STAT6

LV-

LV+

CUX-

CUX+ LV-

LV+

CUX-

CUX+

Nucleus Cytoplasma

CUX1

MEK1

ORC2

B

Suppl. Fig. 3

Suppl. Fig. 4

human

mouse -200 ACCTCACTCGTTAAAAATTAAAAGGAGCACAAGAGGGG-AGAGGGAAATTCC--AAGTTCATGGGTCACAATAAACACAAGCAATGCCCTCG-GTTT---ACAGGGGACTTCCCTCGGGT

-212 ACCTAATTCACTATTACCAAAAA--------------AAGAGGAGCAGAGGGAAATTCCGTAACTTGGAGGCT-ACAATAAATA------ATACCTTCGAGTCTGCAACATGGGACTTCCCCAGGA----

human

mouse -------T-GC--GGAGCCTT-GCTGAGTCATCTCCAAAGTC---AGCCAATCAGGACTCAGGGAGGGAAACT--CTTTGCAGATAAATA---CTC -10

ACAGCCAGCAGGTTTTGCTAAGTCAACTGTAATGCCCTTATCCAATCAGAATT-AGGGAGGGAAAATGGCTTTGCAGATAAATATGGCAC -18

CXCL10 CXCL10-1 for CXCL10-1 rev

CXCL10-2 for CXCL10-2 rev

Oligo-1

Oligo-2

Oligo-2

Green: putative CUX1 binding siteRed: putative NF-kB binding sitesSolid lines: qPCR primer for ChIPDashed lines: Oligonucleotides for DNA-pulldown

Suppl. Fig. 5

A

*

B C

merge

DAPI

Granzyme B

CD8