Super Resolution Imaging Solution Provider

Imaging Future

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NanoBioImaging(NBI) is the Industrial Partner of HKUST Super Res-olution Imaging Center (SRIC). NBI aims to develop and commer-cialize unconventional optical microscope techniques and provides professional, affordable and fast super resolution imaging services for life sciences institutes, universities and the biomedical industry.

Imaging SolutionMore Than Equipment

Super Resolution Imaging - A Door To The Nano-World

Using appropriate dyes, the fluorescent signal decay can be combined with single mol-ecule localization techniques to achieve 20 nm resolution. The NBI super resolution (SR) imaging system achieves a 10-fold improvement compared to conventional confocal microscopy. It allows nanoscale quantification of protein localization or protein-pro-tein interaction. The super resolution microscope can be used for biological research in cell biology, neuron biology, immunology, virology and nanomaterials.

Two-color super resolution imaging of Al-exa750-anti-GFP nanobody labeled Tub1 of spindle microtubule and Alexa647-an-ti-RFP nanobody labeled Tub4 of spindle pole body in a yeast cell. (a) and (b) are Epi fluorescent images where (b) is the zoom in of the marked area in (a). (c) and (d) are the two-color super resolution images at the same position where the anchoring of spin-dle microtubules to spindle pole bodies can be clearly resolved.

(Collaboration with biochemistry group from HKUST)

Synaptic structure in tissue slice resolved by localization microscope. (a)(c) and (e) show the EPI-fluorescent image which fails to show structural details. (b)(d) and (f) are super res-olution images acquired by NBI two-color lo-calization microscope. The pre- and post-syn-aptic structures are clearly resolved.

(Collaboration with biochemistry group from HKUST)

Yeast Organelle

Neuron Synapse

Two-color super resolution imaging re-solves p32 (mitochondrial matrix) packed by Tom20 (mitochondrial outer membrane) in HEK293 cell line. (a) Epi fluorescent image with totally blurred structure. (b) The super resolution images at the same location. (c) The transverse profile correspondence to boxed region in (b), clearly shows that the p32 is packed inside the mitochondrial out-er membrane.

(Collaboration with biochemistry group from HKUST)

Mitochondrial Membrane Structure

Widefield Image Super Resolution Image

Two-color super resolution imaging re-solves GM130 (cis-Golgi matrix protein) and Golgin97 (trans-Golgi network membrane protein) in HeLa cell line. (a) Epi fluorescent image with blurred strucutre and more over-lapping regions of the two proteins. (b) Su-per resolution image at the same location where two layers (cis vs. trans) in golgi appa-ratus are better distinguished.

(Collaboration with biochemistry group from HKUST)

Three-dimensional super-resolution imag-ing of F-actin network in Drosophila S2 cell, with the z-position information color-coded according to the color scale bar.

(Collaboration with biochemistry group from HKUST)

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F-actin Network Structure

Golgi Apparatus

1000 nm

a b

1 μm

200 nm 200 nm 200 nm

1 μm

200 nm

How to Obtain The Best SR Imaging Result?

Stable and Efficient Hardware

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Local and Instant Tech-support

Easy and Convenient Software

Super Resolution Imaging Plan

Exclusively For You

NBI-SR

Widefield

Professional and Accurate Analysis

Customized & Upgradable System Design

Professional & ComprehensiveService Support

Super resolution imaging is a combination of physics, chemistry, biology, computer science and engineering technology. This interdisciplinary nature can make it challenging for biologists (experimental design, sample preparation, equipment calibration, software configuration, data analysis). In collaboration with HKUST SRIC, NBI has built a team that provides customized hardware, software and comprehensive technical support. Ultimately our customers benefit when they get the best results with minimal effort.

Stable and Efficient Hardware

System Specifications

Super-Resolution ~ 20 nm in lateral

~ 50 nm in axial

Compatible Laser 405 nm, 488 nm, 656 nm, 750 nm and others upon demand

Color Channels TTwo channel imaging simultaneously

Compatible Objec-tive

CFI Apochromat TIRF 100×oil (NA1.49)

CFI Plan Apochromat VC 100×oil (NA1.40)

Compatible Micro-scope ECLIPSE Ti-E

Camera Andor iXon 897 EMCCD

Active Pixels 512×512

Pixel Size 16 microns

Readout Noise < 1e-

Autofocus Perfect Focus System

Lateral Locking LED based Active Locking, std ~ 1nm

Anti-vibration Optical table with passive isolator

Environment General lab conditions

Dye Alexa 647, Alexa 750 and others upon demand

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Architecture of the super resolution imaging system developed by NBI:

The intermediate image is split and imaged on EMCCD sensor through two separate optical paths simultaneously. Compared with gener-al super resolution microscopy, long term ac-quisition of super resolution imaging benefits from the two channel design, which reduces photobleach and halves the imaging time.

Real-time locking system compensates lateral sample drift in caused by environ-mental conditions (temperature change, air flow, vibratior noises). Compared with post processing techniques used by others, the sample locking module designed by NBI can compensate this drift at nanometer res-olution with high reliability.

EFFICIENT Two-Channel Imaging Simultaneously

STABLEHigh-Resolution Sample Locking

Convenient and User-Friendly Software

Automatic User & Data Management

Real-time Monitoring of Reconstruction with Details

ROHDEA maintains all data for each user in an intelligent and well-organized structure, including raw images and all useful parameters and computed/reconstructed results. ROHDEA also records configuration of arguments for similar experiments in the future.

Streamlined Image Acquisition

ROHDEA is software developed by NBI for super resolution imaging. It makes using the system both easy and efficient for our users.

Imaging Result Analysis

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ROHDEA provides improved user experience compared with competing imaging software solu-tions that are function-oriented and are harder to use. ROHDEA structures super resolution imag-ing workflow into four steps: 1. view preparing, 2. wide-field image acquisition, 3. super resolution image testing and 4. super resolution image acquisition. This standardized sequence of steps is easier to follow compared with traditional approaches.

ROHDEA embeds multiple super resolution imaging algorithms, and presents real-time reconstructed images. Parameters can be changed in real-time to op-timize the result.

Users can analyze the reconstructed super resolution images with SPRUCE, an image analysis tool also developed by NBI, to get customized analysis reports. Analysis of the result can provide useful information such as the distribution of cluster sizes/patterns, localization distribution, error distribution etc.

Easy to Test Arguments for Reconstruction

The parameters for the reconstruction algorithms will affect the result quality. The testing step offers user an easy and visualized way to test different arguments for better configuration.

Simplicity is the soul of efficiency

Professional and Comprehensive Services

Experimental Design - Super Resolution Or Not

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The ultimate goal of microscopy is to achieve high quality images. NBI imaging service will take you there with minimal time and effort.

Judging whether a new technology will be useful for a particular application requires experience. NBI can give you the answer rapidly, with our academic strengths and our knowledge base in the field. We also customize an imaging plan for your project. You can also choose our imaging service, with which you can test your hypothesis within weeks. Contact us now to discuss your imaging project!

Consultation - Your Problem Is Our Problem

Hardware after-sales service doesn't just mean hardware warranty -- helping you run your machine is also considered to be an important part of NBI service.

Our consultation service includes trouble shooting, sharing technical experience, data analysis and modeling, statistical analysis, etc.

Training - More Than Operation

Following a standard "step-by-step" manual process can produce the optimal result for a demo sample. However your particular samples may require a different approach. NBI's scientists in different fields can provide professional training to the users, so they know which technique will work best for their situation.

NanoBioImagingHelp Know Why, Not only How

Long Term Collaboration - Make Progress Together

Leading edge technologies can provide an advantage in research.In our long-term technical collaboration, our customers have priority access to our most advanced technologies. Furthermore, NBI also provides customized solutions for your research needs.

Unit 623, 6/F Biotech Centre 2, No.11 Science Park West AvenueHong Kong Science Park, Shatin, N.T., Hong Kong

Tel: (852)2688 0511 (86) 14716022251http://www.nanobioimaging.com

Email: contact@nanobioimaging.com

Imaging Future

Specifications and appearances are subject to change without notice or obligation on the part of the manufacturer.