7 Subject index

Accessory cell groups 12ff- vasopressin 12ff- - in adult rats 12,14- - in infantile rats 17, 21, 27ff- - in post partum rats 72- - in thirsting rats 83Amygdala 37, 47, 122f- vasopressin 2Annular granules 21, 25, 27f, 119Antibody, titer 114Arcuate nucleus. See nucleus arcuatusArea postrema 111- preoptica, luliberin 3Axonal swellings, Brattleboro rat 136- - thirsting rat 84ff, 134ff- - vasopressin, post partum rat 78

Calleja islets 36, 122fCircumventricular organs 1, 142ffCRF-activity, vasopressin 2, 119, 139ff

Development, LRF system 130fDiestrus, EM, somatostatin, immobilization

stress 102- - vasopressin, immobilization stress 98- LRF, thirsting rat 93

OVLT, somatostatin, immobilization stress104f

Eminentia mediana. See median eminenceEstrus, EM, somatostatin 103f- - vasopressin 98- OVLT, somatostatin, immobilization stress

104fExtrahypothalamic projections,

neurohormones 117

Granules, annular 21,25, 27f, 119

Hypothalamohypophyseal tract 12f- vasopressin 2, 113, 116£- - in adult rats 10ff- - in immobilization stressed rats 17,21,29- - in post partum rats 78- - in pregnant rats 72, 130f

in thirsting rats 84- - seasonal variations 15

Immobilization stress 5, 123Interstitial nucleus, stria terminalis 37

LH-RH. See luliberinLRF. See luliberinLuliberin, cycle variations 58- experimental stress 4- in adult rats 58ff, 64- - immobilization stressed rats 107f, 142- - infantile rats 130f- - perikarya 58f, 128f- - post partum rats 79ff, 133f- - pregnant rats 76ff, 132f- - thirsting rats 91ff, 137f- mamillary nuclei 4- nucleus arcuatus 58, 128f- postnatal development 4- preoptic area 3, 58, 126, 128f- projections 129f- seasonal differences 58, 61ff- sexual differences 58Luliberin-system 1, 3f, 58ff, 115, 126ff- development 63ff, 130- differences in stainability 128f, 132fLuteinizing hormone releasing factor. See

luliberin

Median eminence, EM 1- development 125- luliberin 3f, 127ff- - in adult rats 58ff, 64- - in immobilization stressed rats 96ff, 107f- - in infantile rats 63ff, 68, 130f- - in post partum rats 79ff- - in pregnant rats 76ff- - in thirsting rats 91 ff- neurohemal contact zone 4- portal vessels 3f- section planes 8- somatostatin 3f- - in adult rats 37ff- - in immobilization stressed rats 99ff- - in infantile rats 49f- - in post partum rats 79- - in pregnant rats 74ff- - in thirsting rats 89f- staining intensity 8f- vasopressin 2, 116

162 . BRIGITIE KRISCH

Median eminence, vasopressin (Continued)in adult rats 12, 16in immobilization stressed rats 96ff, 139ffin infantile rats 15ff, 21ff, 26ff, 32, 118in post partum rats 78ffin pregnant rats 72, 130fin thirsting rats 84ff

- - seasonal variations 15Methods, electron microscopy 6- light microscopy sf

Neural lobe 1- vasopressin 116

in adult rats 12f, 18- - in immobilization stressed rats 93ff, 138ff- - in infantile rats 19ff, 24ff

in post partum rats 79- - in pregnant rats 72ff- - in thirsting rats 86£- seasonal variations 15Neuroendocrine systems, individual variations

115fNeurohemal regions 1, 109ff, 124, 142Neurohormone, release 113f- transport 113fNeurohormones, extrahypothalamic

projections 112Nuclear areas If- vasopressin 10f

in adult rats 1offin immobilization stressed rats 93ff, 138ffin infantile rats 17, 21, 27ffin post partum rats 78, 133in pregnant rats 70ff, 130in thirsting rats 81 ff, 134f

Nucleus arcuatus 35, 37, 40, 110f- - LRF 3,137- - somatostatin 3, 122f- mamillaris medialis 100, 112- paraventricularis, vasopressin If, 116- - - in adult rats 12- - - in immobilization stressed rats 93ff- - - in infantile rats 21, 26ff

in post partum rats 78- - - in pregnant rats 70ff, 130- - - in thirsting rats 80ff- premamillaris ventralis 35, 37,42- preopticus 3, 35f, 122- suprachiasmaticus 129, 140- - somatostatin 35, 39

- - vasopressin If, 116£- - - in adult rats 15,22Nucleus suprachiasmaticus, vasopressin

(Continued)in immobilization stressed rats 98fin infantile rats 21, 27, 29ffin pregnant rats 74in thirsting rats 88

- supraopticus, vasopressin If, 116- - - in adult rats 10ff- - - in immobilization stressed rats 93ff- - - in infantile rats 17,21, 26ff- - - in post partum rats 78- - - in pregnant rats 70ff, 130- - - in thirsting rats 80ff- ventromedialis 35, 40, 122f

Olfactory tubercle 36£, 44Organum vasculosum laminae terminalis 1,4- blood supply 122- development 56£- luliberin 4, 115, 126

in adult rats 50, 61 ff, 66in immobilization stressed rats 109in infantile rats 67f, 130fin post partum rats 80fin pregnant rats 76ffin thirsting rats 92f

- somatostatin 3f, 115in adult rats 45fin immobilization stressed rats 104ffin infantile rats 56ffin post partum rats 79fin pregnant rats 76in thirsting rats 89ff

Ovarian cycle 4, 37EM, LRF 107, 129EM, somatostatin 99LRF 4, 61OVLT, LRF 127somatostiltin 3, 37

- - vasopressin 2, 10

Paraventricular nucleus. See nucleusparaventricularis

Paraventriculohypophyseal tract 12, 27PAP-method, interpretation 112f- limits of, 112fPars tuberalis, adenohypophysis 96- - vasopressin 132, 139f

Immunocytochemistry of Neuroendocrine Systems . 163

Periodic acid disulfite aldehyde thionine(PBA) method 6£, 56

Peroxidase studies 7Pituicytes 21, 25Premamillary nucleus. See nucleus

premamillarisPreoptic area 123- - LRF, thirsting rat 137fPreoptic nucleus. See nucleus preopticusProjections, extrahypothalamic,

neurohormones 117

Recording of findings 114fRelease, neurohormone 113f

Seasonal differences, EM, somatostatin 88- - OVLT, somatostatin 91- variations 15,37,41- - EM, LRF 61, 127ff- - OVLT, LRF 63, 93,129- - vasopressin 2, 81,117,135,137Sexual differences, EM, LRF 63,67, 100, 107,

142- -, - somatostatin 99, 124- -, - vasopressin 31, 96ff- - OVLT, LRF 63, 67- -, - somatostatin 104f, 124, 104f, 124Somatostatin, cycle phases 37, 45, 49- effector hormone 132- in adult rats 33ff, 41, 48- - immobilization stressed rats 99ff, 141f- - infantile rats 49ff, 124f- - pregnant rats 74ff, 132- - thirsting rats 88ff, 137- nucleus arcuatus 122f- nucleus premamillaris ventralis 122f- nucleus suprachiasmaticus 122f- nucleus ventromedialis 122f- olfactory tubercle 122f- postnatal development 3- seasonal phases 37f, 45, 49- sexual differences 37, 45, 49, 54, 56£Somatostatin-fiber projections 35ff, 122ff- - descending projections 37- - dorsolateral projections 37, 46- - hypothalamic projections 35ff, 122ff- - rostral projections 35ff

- - ventrolateral projections 37, 46£Somatostatin-LRF antagonism 13fSomatostatin-perikarya, in immobilization

stressed rats 99- - in infantile rats 49ff, 53, 55- - in post partum rats 79, 133- - in thirsting rats 88Somatostatin-system Iff, 31ff, 115, 120ff- - plasticity 121f, 133Staining intensity, EM 8fStria terminalis 2, 36£, 43, 122f- - interstitial nucleus 37Subfornical organ 36, 44, 111, 122fSupraoptic nucleus. See nucleus supraopticusSupraopticohypophyseal tract 10, 27Swollen axons, post partum rat, vasopressin 78- - thirsting rats 134ff

Tanycytes 109ff, 142ffThirst stress 5Transport, neurohormones 113f

Vascularization, development, EM 19ff, 25,124f

-, - nuclear areas, 17,21,27-,- OVLT56£Vasopressin, amygdala 2- CRF-activity 2, 119, 139ff- cyclic variations 2- in adult rats 10ff, 15f, 18,22, 45f, 50- - immobilization stressed rats 93ff, 138ff- - infantile rats 15ff, 21, 23f, 26, 28, 32, 118- - post partum rats 78f- - pregnant rats 70ff, 131f- - thirsting rats 2, 81ff, 88, 134ff- - intercellular clefts 139f- multiple functions 2- non-granule bound 83ff, 94, 135ff, 141- pars tuberalis, adenohypophysis 132, 139f- seasonal variations, 2, 20, 81, 117, 135, 137Vasopressin-system 10ff, 115ffVessels, neurohemal regions 11 offVentral premamillary nucleus. See nucleus

premamillaris ventralisVentromedial nucleus. See nucleus

ventromedialis