study title - pineapple uk...study title: measurement of antiviral activity on plastics and other...

Test identification Reference: J001515

BS ISO 21702:2019

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Study Title:

Measurement of antiviral activity on plastics and other non-porous surfaces

Microbiological Solutions Limited (MSL)

Gollinrod, Walmersley, Bury, BL9 5NB, UK

Angela Davies, CEO

Customer: Pineapple Contracts Contact name: Glyn Hathorn Email: [email protected] Address: Westmead, Aylesford, Kent, ME20 6XJ PO/Quote number: Q002344

____________________ ____________________ Megan Barrett Peter Thistlethwaite Laboratory Manager Technical Projects Manager

The test results on this report refer only to the items tested as supplied by the customer. This report shall not be reproduced

except in full and with written approval of Microbiological Solutions Ltd. All reports are archived for a minimum of 2 years. The sample will be retained for 1 month unless otherwise requested in writing.


BS ISO 21702:2019

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Scope

The standard describes the method for measuring antiviral activity on plastics and other non-porous surfaces of antiviral-treated products against specified viruses. Outline of Test Method (Obligatory Test Conditions) A test suspension of the virus is inoculated onto a test plastic surface and covered with a cover film. The surface is maintained at a specified temperature for a defined period. At the end of the contact time media is added to the surface of the plastic, and the surface is washed over to recover any remaining organism. The number of surviving organisms which can be recovered from the surface is determined quantitatively taking in to account the test surface size. Coronavirus summary


BS ISO 21702:2019

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Deviations from Standard Method

Test Result Summary

Test information Deviation Name of Product 1) Ryno

2) Scorpio 3) Sovie

Batch Number & Expiry Date N/S

Date of Delivery 26/03/2020

Period of Analysis 27/03/2020-31/03/2020

Manufacturer / Supplier Pineapple contacts

Storage Conditions Ambient

Appearance of the Product 1) Ryno – Multicoloured plastic tiles 2) Scorpio – Blue material 3) Sovie – White wood

Test Concentrations As supplied Test Temperature 20°C ± 1°C Temperature of Incubation 37°C ±1°C Identification of the Viral Strains: Feline coronavirus, Strain Munich 1

Contact Times 24 hours Stability and Appearance During Test No Change Observed

The test products received have received the following log reductions (resistance to growth) against Feline coronavirus:

1) Ryno – Multicoloured plastic tiles 0.65 log reduction 2) Scorpio – Blue material 0.58 log reduction 3) Sovie – White wood 0.45 log reduction

See page 2 for outline of the test method and for raw data tables below for complete test results.

1 - The test surface was challenged against Feline coronavirus. 2 – No control material was supplied so samples were compared to glass slides. All samples were visibly soiled upon receipt. In order to test with the required sterility, samples were wiped with 70% alcohol wipes and allowed to dry before testing. Calculation notes All recovery and log reduction calculations were performed for TCID50 rather than plaque assays. Cytotoxicity of the test product was performed through adding 10ml of culture media and washing the surface, this solution was then added to cells in serial dilution and cytotoxicity calculated by TCID50. Log recovery per surface is calculated and an average reduction given.


BS ISO 21702:2019

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Summary

Reduction test 1

Reduction test 2

Reduction test 3

1 2 3 Average

Test 1 2.92 3.04 3 2.99 At 4.99

Test 2 3.04 3.04 3.08 3.05 At 5.05

Test 3 3.17 3.25 3.13 3.18 At 5.18

Control (t) 3.66 3.83 3.42 3.64 Ut 5.64

Control (0) 5.29 5.71 6.13 5.71 Uo 7.71

Log recovered per surface

Log recovery

Antiviral activity per surface (R)

0.65

R=(Ut-Uo)-(At-Uo)

R=(Ut-Uo)-(At-Uo)


0.58

R=(Ut-Uo)-(At-Uo)


0.45


BS ISO 21702:2019

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Controls

Cytotoxicity (Test 1) Negative



Cytotoxicty (Control) Negative

Difference Valid

Test 1 St 4.17 0.16 Y

Test 2 St 4.21 0.12 Y

Test 3 St 4.25 0.08 Y

Control (Untreated) Su 4.29 0.04 Y

Negative control Sn 4.33 N/A Y

Log recovered

Inactivation control


BS ISO 21702:2019

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Raw data

Test 1 R1 Organism Feline Coronavirus

Dilution Counts % CPE p(1-p) Strain Munich

-2 4 4 4 4 4 4 1 0 d 1

-3 1 1 2 2 1 1 0.33333333 0.222222 sum px 1.42

-4 1 1 0 0 0 0 0.08333333 0.076389 n 8

-5 0 0 0 0 0 0 0 0 SD50 -2.92

-6 0 0 0 0 0 0 0 0 SE 0.21

-7 0 0 0 0 0 0 0 0 xp -2

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 2 2 3 1 1 1 0.41666667 0.243056 sum px 1.54

-4 1 1 0 0 0 1 0.125 0.109375 n 8

-5 0 0 0 0 0 0 0 0 SD50 -3.04

-6 0 0 0 0 0 0 0 0 SE 0.22

-7 0 0 0 0 0 0 0 0

-8 0 0 0 0 0 0 0 0 xp -2

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 2 2 2 1 1 1 0.375 0.234375 sum px 1.50

-4 1 1 1 0 0 0 0.125 0.109375 n 8

-5 0 0 0 0 0 0 0 0 SD50 -3.00

-6 0 0 0 0 0 0 0 0 SE 0.22

-7 0 0 0 0 0 0 0 0 xp -2

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0


BS ISO 21702:2019

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Raw data



-2 4 4 4 4 4 4 1 0 d 1

-3 3 3 2 2 1 1 0.5 0.25 sum px 1.54

-4 1 0 0 0 0 0 0.04166667 0.039931 n 8

-5 0 0 0 0 0 0 0 0 SD50 -3.04

-6 0 0 0 0 0 0 0 0 SE 0.20

-7 0 0 0 0 0 0 0 0 xp -2

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 3 3 1 1 1 1 0.41666667 0.243056 sum px 1.54

-4 0 0 1 0 0 2 0.125 0.109375 n 8

-5 0 0 0 0 0 0 0 0 SD50 -3.04

-6 0 0 0 0 0 0 0 0 SE 0.22

-7 0 0 0 0 0 0 0 0

-8 0 0 0 0 0 0 0 0 xp -2

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 3 3 2 2 2 1 0.54166667 0.248264 sum px 1.58

-4 1 0 0 0 0 0 0.04166667 0.039931 n 8

-5 0 0 0 0 0 0 0 0 SD50 -3.08

-6 0 0 0 0 0 0 0 0 SE 0.20

-7 0 0 0 0 0 0 0 0 xp -2

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0


BS ISO 21702:2019

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Raw data



-2 4 4 4 4 4 4 1 0 d 1

-3 3 3 3 2 2 1 0.58333333 0.243056 sum px 1.67

-4 1 1 0 0 0 0 0.08333333 0.076389 n 8

-5 0 0 0 0 0 0 0 0 SD50 -3.17

-6 0 0 0 0 0 0 0 0 SE 0.21

-7 0 0 0 0 0 0 0 0 xp -2

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 2 2 2 2 4 4 0.66666667 0.222222 sum px 1.75

-4 1 1 0 0 0 0 0.08333333 0.076389 n 8

-5 0 0 0 0 0 0 0 0 SD50 -3.25

-6 0 0 0 0 0 0 0 0 SE 0.21

-7 0 0 0 0 0 0 0 0

-8 0 0 0 0 0 0 0 0 xp -2

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 4 3 2 2 1 1 0.54166667 0.248264 sum px 1.63

-4 1 1 0 0 0 0 0.08333333 0.076389 n 8

-5 0 0 0 0 0 0 0 0 SD50 -3.13

-6 0 0 0 0 0 0 0 0 SE 0.22

-7 0 0 0 0 0 0 0 0 xp -2

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0


BS ISO 21702:2019

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Raw data

Control (t) R1 Organism Feline Coronavirus


-2 4 4 4 4 4 4 1 0 d 1

-3 4 4 4 4 3 3 0.91666667 0.076389 sum px 2.17

-4 1 1 0 0 2 2 0.25 0.1875 n 8

-5 0 0 0 0 0 0 0 0 SD50 -3.67

-6 0 0 0 0 0 0 0 0 SE 0.19

-7 0 0 0 0 0 0 0 0 xp -2

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 4 4 4 4 4 4 1 0 sum px 1.33

-4 2 2 1 1 0 0 0.25 0.1875 n 8

-5 1 1 0 0 0 0 0.08333333 0.076389 SD50 -3.83

-6 0 0 0 0 0 0 0 0 SE 0.19

-7 0 0 0 0 0 0 0 0

-8 0 0 0 0 0 0 0 0 xp -3

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 3 3 3 3 4 1 0.70833333 0.206597 sum px 1.92

-4 2 2 1 0 0 0 0.20833333 0.164931 n 8

-5 0 0 0 0 0 0 0 0 SD50 -3.42

-6 0 0 0 0 0 0 0 0 SE 0.23

-7 0 0 0 0 0 0 0 0 xp -2

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0


BS ISO 21702:2019

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Raw data

Control (0) R1 Organism Feline Coronavirus


-2 4 4 4 4 4 4 1 0 d 1

-3 4 4 4 4 4 4 1 0 sum px 2.79

-4 4 4 4 4 3 3 0.91666667 0.076389 n 8

-5 3 3 3 4 2 1 0.66666667 0.222222 SD50 -5.29

-6 1 1 1 1 1 0 0.20833333 0.164931 SE 0.26

-7 0 0 0 0 0 0 0 0 xp -3

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 4 4 4 4 4 4 1 0 sum px 2.21

-4 4 4 4 4 4 4 1 0 n 8

-5 4 4 3 3 3 3 0.83333333 0.138889 SD50 -5.71

-6 2 2 1 1 0 1 0.29166667 0.206597 SE 0.25

-7 1 1 0 0 0 0 0.08333333 0.076389

-8 0 0 0 0 0 0 0 0 xp -4

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 4 4 4 4 4 4 1 0 sum px 2.63

-4 4 4 4 4 4 4 1 0 n 8

-5 4 4 3 3 4 4 0.91666667 0.076389 SD50 -6.13

-6 2 2 1 3 3 3 0.58333333 0.243056 SE 0.25

-7 1 1 0 0 1 0 0.125 0.109375 xp -4

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0


BS ISO 21702:2019

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Raw data

Interference Test 1 Organism Feline Coronavirus


-2 4 4 4 4 4 4 1 0 d 1

-3 4 4 4 4 4 4 1 0 sum px 1.67

-4 3 3 3 2 2 1 0.58333333 0.243056 n 8

-5 1 1 0 0 0 0 0.08333333 0.076389 SD50 -4.17

-6 0 0 0 0 0 0 0 0 SE 0.21

-7 0 0 0 0 0 0 0 0 xp -3

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 4 4 4 4 4 4 1 0 sum px 1.71

-4 2 2 1 3 3 2 0.54166667 0.248264 n 8

-5 1 1 1 0 0 1 0.16666667 0.138889 SD50 -4.21

-6 0 0 0 0 0 0 0 0 SE 0.24

-7 0 0 0 0 0 0 0 0

-8 0 0 0 0 0 0 0 0 xp -3

-9 0 0 0 0 0 0 0 0



-2 4 4 4 4 4 4 1 0 d 1

-3 4 4 4 4 4 4 1 0 sum px 1.75

-4 3 3 2 1 2 1 0.5 0.25 n 8

-5 1 1 2 2 0 0 0.25 0.1875 SD50 -4.25

-6 0 0 0 0 0 0 0 0 SE 0.25

-7 0 0 0 0 0 0 0 0 xp -3

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0


BS ISO 21702:2019

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Raw data

Interference Control 1 Organism Feline Coronavirus


-2 4 4 4 4 4 4 1 0 d 1

-3 4 4 4 4 4 4 1 0 sum px 1.79

-4 4 3 3 3 2 1 0.66666667 0.222222 n 8

-5 1 1 1 0 0 0 0.125 0.109375 SD50 -4.29

-6 0 0 0 0 0 0 0 0 SE 0.22

-7 0 0 0 0 0 0 0 0 xp -3

-8 0 0 0 0 0 0 0 0

-9 0 0 0 0 0 0 0 0

Interference Negative 2 Organism Feline Coronavirus


-2 4 4 4 4 4 4 1 0 d 1

-3 4 4 4 4 4 4 1 0 sum px 1.83

-4 3 3 3 3 3 3 0.75 0.1875 n 8

-5 1 1 0 0 0 0 0.08333333 0.076389 SD50 -4.33

-6 0 0 0 0 0 0 0 0 SE 0.19

-7 0 0 0 0 0 0 0 0

-8 0 0 0 0 0 0 0 0 xp -3

-9 0 0 0 0 0 0 0 0


BS ISO 21702:2019

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KEY CPE Cytopathic effect Counts 0-4 indicating degree of cytopathic effect 0 = No effect, 1 = 25% CPE, 2 = 50% CPE, 3 = 75% CPE, 4 = 100% CPE d Dilution factor (log) Sum px Sum of % CPE from the highest dilution showing 100% CPE to the lowest dilution assessed. n Number of dilutions SD50 Dilution showing 50% of the end point according to Spearman-Kärber method SE Standard error xp Lowest dilution showing 100% CPE TCID50 Titre causing 50% of the end point according to Spearman-Kärber Sn Log of negative control Su log of untreated control St Log of test control At Log of treated test Ut Log of untreated control at time Uo Log of untreated control at time 0 R Log reduction

study title - pineapple uk...study title: measurement of antiviral activity on plastics and other...

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