Archives of Virology

Detection, discrimination and quantitation of 22 bluetongue virus serotypes using real-time RT-PCR with TaqMan MGB probes

Yufei Feng1, 2, Tao Yang2, Qingyuan Xu2, Encheng Sun2, Junping Li2, Shuang Lv2, Haixiu Wang2, Qin Zhang2, Jikai Zhang2, Donglai Wu1, 2*

1 Department of Basic Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, P. R. China 2 The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P. R. China

*Corresponding author: E-mail: dlwu@hvri.ac.cn (D. Wu); Tel: +86-189-4606-6088

Supplementary Material 1

Supplementary Material 1 BTV serotype-specific TaqMan-MGB probe target sequences are highly conserved among each BTV serotype. Segment-2 nucleic acid sequences were aligned for all available BTV segment 2 sequences available in GenBank to identify highly conserved regions within each BTV serotype. Shown by BTV serotype is the selected TaqMan-MGB probe target sequence and the corresponding sequence found in a series of BTV isolates from the corresponding serotype.