sputum examination
TRANSCRIPT
Investigation Seminar
Sputum ExamiNAtionBy
Dr.Ajay Chavan
104/12/09
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OBJECTIVE
Production of Sputum . Collection of sample. Examination – Macroscopic . Microscopic . Conclusion .
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PRODUCTION OF SPUTUM Sputum is produced by a spontaneous
deep cough bringing up material from
small airways and alveoli.
Sputum is basically a product of secretions
of mucous glands & goblet cells in the
bronchial wall. It's the cellular material
from the respiratory tree.
95% water,5%solids
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COLLECTION OF SPECIMEN1. Before collecting or expectorating sputum
the mouth should be prerinsed and this
removes contaminants from oral cavity.
2. For best results early morning freshly
expectorated sputum specimen should be
collected in dry sterile bottle.
A cough plate is held before the child’s
mouth & the child’s urged to cough.
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CONT ….
Cough swab method gives the most
representative, non contaminated sputum
sample. Child’s mouth is held open by using
tongue depressor. Material expelled from
Trachea is deposited on the swab, which can
then plated on the appropriate culture media.
In pts who are uncooperative or cannot
produce adequate sputum, induction should
be tried.
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D
IFFE
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SPU
TU
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7SPUTUM IN THE CONTAINER
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EXAMINATION
Specimen should be collected in a sterile,
disposable , impermeable container.
Transfer specimen in sterile petridish placed
against dark background.
Wooden applicator sticks can be used to
spread it thinly & can be seen by naked eye
or by using lens.
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CONT ….
A. MACROSCOPIC OR BEDSIGHT
B. MICROSCOPIC OR IN LABORATERY
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MACROSCOPIC
VOLUME-
A 24 hr volume of sputum is measured in
pt with chronic bronchitis, lung abscess or
bronchial asthma. A rising volume indicates
worsening & decreasing volume indicates
improvement.
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CONT …. COLOUR-
Watery or Frothy = pulmonary oedema
Green= Early stage of T.B.
Yellow= Brochiectasis
Blood in sputum= T.B., Ca of lung
Pink= lung oedema
White= mucoid
Black= excess ‘C’in respiratory track
Rusty= lobar pneumonia (Streptococcal)
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CONT….
ODOUR-
Normal sputum is odorless
Suppurative smell= pulmonary
disorders
Fecal odour= liver abscess
Intolerable smell= gangrene of lungs
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CONT ….
VISOSITY-
Mucoid sputum has high viscosity
Purulent sputum has low viscosity
Mucopurulent has intermediate viscosity
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CONT ….
APPEARANCE-
Serous frothy= bronchioalvolar CA
Nummular sputum= pulmonary TB
Thick viscid sputum= asthma
Thin watery sputum= pulmonary
oedema
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MICROSCOPIC
After macroscopic examination
transfer material to a clear slides.
Smears made on clear slides should be
air dried, fixed over flame and stained.
Then examine under the microscope.
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16SPUTUM SLIDE
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CONT ….
Diff stains used:-
Gram’s stain/ Ziehl-Neelsen stain = for
AFB
Wright’s stain= for blood cells
Buffered crystal violet= for epithelial
cells
Pap’s stain= cytology of sputum
Usual peripheral smear stain= for
blood cell
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Gram’s Stain
Smear the sputumStained by
Gentian violet stain Keep for 3 min.
Bacteria get violet colourPour
Gram’s IodineFor 1 min.
Wash with Alcohol
Wash with water and dry
Mount under oil immersion
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Ziehl Nielsen's Stain
Smear the sputumFixed by Heating
Pour carbol fuchin and heat it from belowKeep for 5 min.
Wash with water
Decolorize with 20%H2SO4Wash with
Loffler's methyene blue for 1 min.Wash &dry
Mount under oil immersion
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SPUTUM CULTURE
Each specimen received for culture should be plated on agar.
Diff agars:-
Blood agar
Chocolate agar
MacConkey’s agar
Thioglycollate broth
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CLASSICAL REFERENCES
Pitavarna ,Kaphasthibana – Pittaj Kasa.(Ch.chi. 18\14)
Madhur Snigdha Ghana – Kaphaj Kasa.(Ch.Chi. 18\17)
Pita Shava Sashonita – Kshataja Kasa.(Ch.Chi 18\19)
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CONT ….
Duraghanda Harita – Kshayaja Kasa Rakta Puyamapi (Ch.Chi.
18\25) Shonita Drashana (Raktasthibana) –
Rajayakshama.
(Ma.Ni.Rajayakshama \2,6,7) Shonita Shleshamana Chardi – Ekadasha
rupa of Rajayakshama.
(Ch.Chi.8\43)
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CONCLUSION
Sputum sample are easily obtained is the
advantage.
Sputum have cellular contain which
represented the entire Respiratory tract.
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REFERENCES
Chamberlain’s symptoms & signs in clinical medicine
Medical laboratory technology-Dr. Sood Index of differential diagnosis Harrison’s Internal Medicine Text book of pathology- Harsha Mohan Hutchison’s Charka Sahita Chikastastana Madava Nidana www.Scribed.com
Thank
You27
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