space seed experiment “life cycle of higher plants under microgravity conditions”

1 Training Purposes OnlyJEM SPACE SEED 31407JMU-081220 (Ver. 1.1)

Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA).

Space Seed Experiment“Life Cycle of Higher Plants under Microgravity Conditions”

JEM SPACE SEED 31407

Instructor: Takashi YamasakiToru Mori

([email protected])([email protected])


Objectives

Space Seed experiment overview

Space Seed experiment operations flow

Key points during Space Seed experiment operations

Space Seed experiment operations

Explain the science objective of Space Seed experiment.

List onboard outcomes from performing Space Seed experiment.

Explain function of Plant Exp Unit.

Explain function of Exp Laptop Terminal (ELT).

Explain Space Seed operations flow regarding Plant Exp Unit.

Explain function of KSC Fixation Tube (KFT).

Explain what crew must check after attaching Plant Exp Unit on CBEF Turntable.

Explain what crew must do in case fragments of plant tissue fly out of Glove Bag.

Explain what crew must check before opening KFT Ziplock Bag.

Explain what crew must take care while inserting plants in KFT.

Explain what crew must take care while taking Plant Exp Sample Chamber out of Plant Exp Unit.

Identify where crew must cut while harvesting plants.

Explain what crew must not do while inserting plants in KFTs to prevent cross-contamination.

Perform Space Seed experiment operations according to ODFs without instructor’s assistance.

After this lesson the trainee will be able to:


Lesson Outline

Contents:

- Space Seed Experiment Overview

- Space Seed Experiment Operations Flow

- Key Points during Space Seed Experiment

- Appendix

Page:

4

8

10

11


Space Seed Experiment Overview

Plants grown in Space (Arabidopsis thaliana) - Plant Exp Sample Chamber (4) - Plant Exp Sample Chamber Bottom (4) - KFT (12) (Kennedy Space Center Fixation Tube) (RNAlater (4), Formaldehyde (2), Fromalin Acetic Acid Ethanol (2), Glutaraldehyde Paraformaldehyde (2), Glutaraldehyde Paraformaldehyde for Long Term (2))

Environment Gas - Plant Exp Gas Sampling Syringe (2)

Data - Temperature/Humidity Data - Video Images - Radiation Data - Microgravity Environment Data

To study Life Cycle of Higher Plants under Microgravity Conditions.

Space Seed experiment science objective

Onboard Outcomes from Space Seed experiment


Space Seed Experiment Overview (cont’d)

Plant Exp Unit- Plant Exp Unit is a kind of CBEF Canister to grow plants.- Plant Exp Unit has following components. - Plant Exp Sample Chamber - LEDs for growing plants - LEDs for lighting and a video camera - Water bag, Water Sensor and Water Supply Pump - Temperature/Humidity Sensor, Ventilation Pump, Heater and Circulation Fan - Plant Exp Sample Chamber also has following components. - Bio Dosimeter - Temperature Logger

Bio DosimeterTemperature Logger

LED for growing plants

Video Camera

Water Bag

Ventilation Pump

Water Supply Pump

Circulation Fan

Temperature/Humidity

Sensor

Water Sensor

Heater

Plant Exp Chamber

LED for lighting


Exp data files

Plant Exp Unit

Cell Biology Experiment Facility (CBEF)

Installed in CBEF Incubator Units

GroundPlant Exp Unit activation/deactivation Commands

Plant Exp Unit on/off status, Temp of circuit board

Exp Laptop Terminal (ELT)(Think Pad A31p)

ELT Data C

able

Temperature/Humidity of Sample Chamber, Pump/Fan status, etc.

Communication Check

Experiment start/s

top,

etc.

Parameter files

Plant Exp Units in CBEF Micro-G Incubator Unit

Plant Exp Units in CBEF 1G

Incubator Unit

Exp Laptop Terminal

Space Seed Experiment Overview (cont’d)

- Exp Laptop Terminal is a laptop computer for controlling Plant Exp Unit in CBEF.


Intentionally Blank


CBEFPlant Exp Unit

:Crew operations

:Ground operations

LEGEND:

CBEF Activation/Environment Control

Plant Exp Unit Activation

Experiment Start by ELT

Plant Exp Unit Installation (8)

-Mic

rogr

avity

Env

ironm

ent

Dat

a

Exp Laptop Terminal

Measurement Start

Space Seed Experiment Operations Flow

Dehumidifier Installation ELT Setup/Activation

MMA

Experiment Stop by ELT

Plant Exp Unit Deactivation

Plant Exp Unit Removal (4)

Harvest (Short/Long Term) CBEF Deactivation

Dehumidifier Removal ELT Deactivation/Removal

Measurement Stop

Rep

ea

t fo

r L

ong

Te

rm E

xpe

rim

en

t

MLT Activation

MLT Deactivation

MELFI Insertion

Tasks just before or during Long Term Experiment

Dehumidifier Exchange

Water Supply/Gas Sampling

ELT Check (periodically)

-Tem

pera

ture

/Hum

idity

Dat

a-V

ideo

Imag

e-R

adia

tion

Dat

a

-Cul

tivat

ion

(S

hort

Ter

m: a

ppro

x. 3

0 da

ys)

(Lo

ng T

erm

: app

rox.

60

days

)

Exp Laptop Terminal

Plant Exp Unit

After Long Term Experiment

After Long Term Experiment

Dehumidifier

Gas Sampling Syringe

PEU Program Installation

Before Short Term experiment


Space Seed Experiment Operations Flow (Cont’d)

Harvest

Take out Sample Chambers from Plant Exp Units

Put Sample Chambers, KFTs, and Harvest Kit in Glove Bag

Open Sample Chamber

Harvest plants

Put plants in KFTs

Fix plants by KFTs

Take out Sample Chambers, KFTs, and Harvest Kit from Glov

e Bag

Glove Bag

Space Seed Harvest Kit

KSC Fixation Tube

Fix plantsPut plants in KFTHarvest plants

Open Sample Chamber

Take out Sample Chamber


Key Points during Space Seed Experiment

NOTE: Underlined key points have been covered by CBEF Advanced Training.

CAUTION: Payload Safety Review Panel has not been completed yet.

Category Equipment Key Points

Crew Safety

Electrical Shock Sample Tray,Centrifuge

Before attaching/detaching the Plant Exp Units to/from the CBEF Sample Tray/Centrifuge, power to Plant Exp Units must be turned off by switches on the Sample Tray/Centrifuge.

Entanglement with rotating device

Incubator Unit1G

Verify visually through the Observation Window that CBEF turntable does not rotate before opening the door of the CBEF Incubator Unit 1G.

Centrifuge When attaching/detaching the Plant Exp Units to/from the CBEF 1G Centrifuge, lock turntable of the Centrifuge by stopper pin.

Release of fragments from rotating device

Centrifuge,Plant Exp Unit

Verify no gap exists between base plate of Plant Exp Unit and CBEF Turntable.

Biomaterial Leakage to Cabin

Glove Bag Vacuum fragments of plant tissues in case they fly out of Glove Bag.

Release of hazardous chemicals

KSC Fixation Tube - Check KFT seals (2 sets of triple O-ring seals on KFT Base Plug and KFT Expansion Plug) have good compression against Main Tube and there are no leaks before opening KFT Ziplock Bag.

- Insert plants into KFT so that they are no longer than the Sample Tube and KFT Actuator O-rings will NOT catch them.

Contamination due to hazardous material leakage to Cabin

CBEF Dehumidifier Verify that there is no leakage before taking out CBEF Dehumidifier from Ziplock Bag.

Time Critical Operation N/A N/A

Dos and Don’ts Incubator Unit Do NOT touch inside of CBEF Incubator Units with bare hand to keep them clean.

Sample Tray,Centrifuge

Be careful not to pinch fingers when releasing drawer locks of slide rails on CBEF Sample Tray/Centrifuge.

Plant Exp Unit Do NOT pull Plant Exp Sample Chamber all the way out of Plant Exp Unit. Or cable/hoses may be snapped.

N/A

(Plant Sample)- Cut under leaves while harvest.- Do NOT insert unidentifiable plants in KFTs to prevent cross-contamination.

Difficulty in Ops N/A N/A

Possible Mis-Ops N/A N/A


Appendix

Appendix

- Harvest for Short Term Experiment- Harvest for Long Term Experiment- Harvest in case of KFT leakage- Acronyms


MELFI-95 deg C

Harvest for Short Term Experiment

Sample Chamber Top (4)

Trash

A sideB side

A sideB side

Half of B side

A side Half of B side

Half of B side

A side Half of B side

Sample Chamber Bottom (4)

KFT RNAlater (4)

KFT Formaldehyde (2)

KFT Formalin Acetic Acid Ethanol (2)

KFT Glutaraldehyde Paraformaldehyde (2)

MELFI2 deg C

CBEF Micro-G Incubator Unit

CBEF 1G Incubator Unit

KFT RNAlater (4)

Relocation5 days later

Harvest for Short Term experiment(approx. 30 days)

Remain in CBEFIncubator Unit for Long Term Experiment

S/N 001

S/N 003

S/N 005

S/N 007


Harvest for Long Term Experiment

Harvest for Long Term experiment(approx. 60 days)



Sample Chamber (4)

Stowed in MELFI from Short Term Experiment

MELFI-95 deg C





MELFI2 deg C

KFT RNAlater (4)Half of B side



KFT Glutaraldehyde Paraformaldehyde for L

ong Term (2)

S/N 004S/N 008

S/N 004

S/N 008

S/N 002

S/N 006


MELFI-95 deg C

KFT RNAlater (3)

MELFI2 deg C



KFT RNAlater (3)

Relocation5 days later

Harvest in case of KFT RNALater leakage


Harvest in case of KFT leakage

- There are 5 KFT RNALater (4 used, 1 spare).- If 1 KFT RNALater has leakage, use spare KFT RNALater.-If 2 KFT RNALater have leakage, plant samples from 2 Plant Exp Sample Chamber A side (S/N 005, 007) will be fixed in 1 KFT RNA Later.

A side

A side

A side

A side

S/N 001

S/N 003

S/N 005

S/N 007


MELFI-95 deg C

B side

B side


MELFI2 deg C




Harvest in case of KFT leakage (Cont’d)

Harvest in case of KFT Formaldehyde leakage

- There are 3 KFT Formaldehyde (2 used, 1 spare).- If 1 KFT Formaldehyde has leakage, use spare KFT Formaldehyde.

S/N 001

S/N 005


- There are 2 KFT Formalin Acetic Acid Ethanol (2 used).- If 1 KFT Formalin Acetic Acid Ethanol has leakage, plant sample from B side of Plant Exp Sample Chamber S/N 007 will be fixed by KFT Glutaraldehyde Paraformaldehyde.

MELFI-95 deg C

Half of B side

Half of B side

B side



MELFI2 deg C





Harvest in case of KFT Formalin Acetic Acid Ethanol leakage

S/N 003

S/N 007


MELFI-95 deg C

Half of B side

Half of B side

B side



MELFI2 deg C





Harvest in case of KFT Glutaraldehyde Paraformaldehyde leakage

S/N 003

S/N 007

- There are 2 KFT Glutaraldehyde Paraformaldehyde(2 used).- If 1 KFT Glutaraldehyde Paraformaldehyde has leakage, plant sample from B side of Plant Exp Sample Chamber S/N 007 will be fixed by KFT Formalin Acetic Acid Ethanol.




Sample Chamber (4)

MELFI-95 deg C


MELFI2 deg C

Half of B side



KFT Glutaraldehyde Paraformaldehyde for L

ong Term (1)

S/N 004


Harvest in case of KFT Glutaraldehyde Paraformaldehyde for long term leakage

- There are 2 KFT Glutaraldehyde Paraformaldehyde for long term (2 used).- If 1 KFT Glutaraldehyde Paraformaldehyde for long term has leakage, plant sample from half of B side of Plant Exp Sample Chamber S/N 008 will be fixed by KFT Formaldehyde spare for short term experiment.

S/N 004

S/N 008

S/N 002

S/N 006

KFT Formaldehyde (1)(Spare for short term)

Half of B side


S/N 008

Stowed in MELFI from Short Term Experiment


Acronyms

CBEF

ELT

KSC

KFT

LED

MMA

MLT

MELFI

ODF

Cell Biology Experiment Facility

Experiment Laptop Terminal

Kennedy Space Center

KSC Fixation Tube

Light Emitting Diode

Microgravity Measurement Apparatus

MMA Laptop Terminal

Minus Eighty degree Laboratory Freezer for ISS

Operations Data File

space seed experiment “life cycle of higher plants under microgravity conditions”

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