Solutions for Biobanking

Products. Expertise. Support.

WhiteSciWhitehead Scientific (Pty) Ltd

Contents

The preservation of biomolecular and/or morphological integrity at the point of sample collection is a crucial aspect in any scientific study. Sample collection solutions should seek to:

• Immediately stabilise samples at the point of collection• Minimise pre-analytical variables to ensure reproducibility• Simplify sample transport logistics following collection• Offer integrated solutions for downstream purification

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Biobanking

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1. Sample Collection & Stabilisation - DNA Genotek ..............................................................................

2. Sample Collection & Stabilisation - QIAGEN .......................................................................................

3. Analyte Purification Solutions ................................................................................................................

4. Solutions for Automation ......................................................................................................................

5. Innovation in Sample Storage Solutions ...............................................................................................

6. Laboratory Sample Management System ...........................................................................................

7. Sample QC & Purity Analysis ................................................................................................................

8. Sample Utilisation & Analysis .................................................................................................................

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Introduction

Biobanking Workflow

Sample Collection and Stabilisation

Oragene•DNA (OG-500) for the collection, stabilisation and transportation of DNA from saliva.

Oragene•DNA (OG-575) for assisted collection – a non-invasive way to collect DNA from infants and young children.

Oragene•RNA for the collection, stabilisation and transportation of high quality mRNA from saliva. RNA is stable at room temperature for months.

OMNIgene•DISCOVER for collecting nucleic acids from saliva for infectious disease sampling. DNA and RNA remains stable for 3 weeks at room temperature.

HEMAgene™•BUFFY COAT for stabilising high molecular weight DNA in buffy coat samples at ambient temperature.

• Improve donor care and compliance with painless, non-invasive sample collection• Eliminate phlebotomy costs• Ideal for use with children or patients that will not comply with blood collections• Samples remain stable for years at room temperature, reducing transportation and storage costs• Samples can be mailed using the standard postal system• DNA from saliva is equivalent to DNA from blood for downstream applications

As researchers strive to discover the causes of disease and identify potential treatments, the acquisition of high quality biospecimens is vital. Reliable long-term stabilisation and storage of biospecimens is critical to the success of biobanks which strive to archive and distribute these valuable genetic resources.

DNA Genotek’s saliva collection kits

Features:

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collection and stabilisation products from DNA Genotek

Biobanking

Sample Collection and Stabilisation

QIAGEN offers an extensive range of products for the immediate stabilisation and subsequent purification of biomolecules

PAXgene Blood DNA System

PAXgene Blood RNA System

PAXgene Blood miRNA Kit

Tissue

RNAlater Stabilisation Reagent

RNAlater TissueProtect Tubes

Allprotect Tissue Reagent• Immediate stabilisation of DNA, RNA, and protein in tissue samples at room temperature• Tissues stabilised at 15-25°C (7 days), 2-8°C (12 months) or -20°C/-70°C (archiving)

• Screw-top tubes containing RNAlater RNA Stabilisation Reagent for added convenience

• Integrated system for the collection, storage and purification of DNA from whole blood • DNA stable at 18-25°C (14 days), 2-8°C (28 days) or -20°C (10 years)

• Isolation and purification of intracellular RNA from whole blood stabilised in PAXgene Blood RNA Tubes• RNA stable at 18-25°C (3 days), 2-8°C (5 days) or -20°C/-70°C (60 months)

• Purification of microRNA and total RNA from whole blood stabilised in PAXgene Blood RNA Tubes• RNA stable at 18-25°C (3 days), 2-8°C (5 days) or -20°C/-70°C (50 months)

• Quickly permeates tissue, stabilising and preserving the RNA expression pattern• RNA stable at 37°C (1 day), 18-25°C (7 days), 2-8°C (4 weeks) or -20°C/-70°C (archiving)• Allows processing, transportation, storage, and shipping of samples without liquid nitrogen or dry ice

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Blood

PAXgene Bone Marrow RNA system

PAXgene Tissue Container

Cells

RNAprotect Cell Reagent

Bone marrow

• For collection, fixation, and nucleic acid stabilisation of human tissues• Novel system to preserve both morphology and nucleic acids• Purification of RNA, miRNA and/or DNA from one sample• Tissue fixation and paraffin embedding without formalin cross-linking• Tissues stabilised at 15-25°C (7 days), 2-8°C (4 weeks), -20°C (months) or -70°C (years)

• Immediate stabilisation and convenient transport of sorted or cultured cells• No need to remove medium• RNA stable at 30°C (1 day), 15-25°C (7 days), 2-8°C (4 weeks) or -20°C/-70°C (archiving)

• Collection of bone marrow samples and stabilisation/ purification of intracellular RNA• Cellular RNA is stable for 18-25°C (3 days), 2–8°C (5 days) and -20°C/-70°C (several months)

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• QIAamp DNA FFPE Tissue kit• RNeasy FFPE Tissue kit• Qproteome FFPE Tissue kit (full-length proteins)• REPLI-g FFPE kit (whole genome amplification)

Analyte Purification Solutions

The purification of high quality samples is a prerequisite to any downstream assay, impacting on assay success rates, reliability and reproducibility.

Efficient purification of DNA

Blood

• PAXgene Blood DNA kit• QIAamp DNA blood kits• QIAsymphony DSP DNA kits• QIAamp 96 DNA QIAcube HT Kit

Tissue

• QIAamp DNA mini kit• QIAsymphony DSP DNA kits

Efficient purification of RNA and/or miRNA

Blood

• QIAamp RNA Blood Mini Kit• PAXgene Blood RNA Kit• QIAsymphony PAXgene Blood RNA Kit

Tissue and cells

• RNeasy and miRNeasy kits• QIAsymphony RNA Kit• RNeasy 96 QIAcube HT Kit

Simultaneous purification of RNA, DNA and protein

Tissue and cells

Tissue

QIAGEN kits maximise yields and quality, ensuring first time success

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Content release from FFPE Tissue

• AllPrep DNA/RNA/Protein mini kit

• Innovative, easy-to-use modular system with built-in touchscreen• Purification of DNA and RNA from a wide range of samples• Process up to 96 samples per run in batches of 24• Continuous sample loading, with bar code reading for sample tracking• Import of sample lists and export of sample sheets

Solutions for Automation

Automation improves lab productivity and frees up valuable time, allowing resources to be allocated to critical tasks. Whatever your requirement, we have a solution to fit your needs.

• Low throughput purification of DNA, RNA, or proteins• Throughput of up to 12 samples per run• Automation of trusted QIAGEN spin-column kits• Elimination of manual processing steps• More free time with affordable automated processing• Standardized results and increased productivity

• Reliable DNA, RNA, and miRNA extraction from virtually any sample type• Mid- to high-throughput solution (up to 96 samples) with dedicated purification kits saves costs and time• User-friendly software allows for easy data management and documentation• Innovative design features increase safety and minimize cross-contamination• Convenient, flexible, and easy-to-use instrument with small footprint

QIAGEN

QIAcube

QIAcube HT

QIAsymphony SP

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Micronic provides traceable sample storage solutions for almost any storage application.

The Micronic portfolio includes a wide range of traceable 2D coded sample storage tubes, as well as code readers for single tubes, plates and racks. A full range of cappers, de-cappers, tube sorters and other tube management systems are available.

Innovation in Sample Storage Solutions

Key features

MICRONIC Traceable sample storage solutions

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• Class 7 cleanroom production• RNase, DNase and endotoxin (pyrogen) free• F.D.A. approved• Sterilisation (ISO 11137)• Sample storage up to 80 years

• Complete Traceability• Laser etched 2D codings: The unique laser encryption code cannot be abraded, fall or be wiped off from the tube• Intuitive and precise tube code reading• Seamless integration into any laboratory sample database• Optimising valuable freezer space• Store your samples over long periods of time without loss of sample integrity• Efficient retrieval of samples• Colour caps for effective means of visually differentiating stored samples

Micronic offers a complete line of sample storage tubes that fit in a standard 96-well or 24-well SBS standard format. This comprehensive line contains tubes from 0.50ml to 7.50ml for your most common storage applications.

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Laboratory Sample Management System

Track-IT is a new Laboratory Sample Management System (LSMS) that allows you to organise and track individual samples in your library. The database is fully customisable to match the workflow of your laboratory, as well as to match the unique aspects of each of your samples.The main concept behind Track-IT is that each scientist in the lab can manage data and information quickly and make it available to the rest of the lab community. Via Intranet support, data can be accessed from any connected computer in the lab, which accelerates information retrieval.Laboratory staff in turn spend less time on recurrent and time-consuming operations and dedicate more precious time to research activity.

Features and benefits

MICRONIC Track-IT Software

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• Central information database• Quick information retrieval• Alerts system• Cross-database searches• Uniform data presentation• Perpetuation of information & Traceability• Data reports export (screen and MS-Excel)• Automatic data imports• Versatile administration• Upgradable• One licence per database covers all lab users

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• Safety and convenience with ready-to-use gel cartridges• Accurate analysis of low concentration nucleic acids with 3-5 bp resolution• User-friendly analysis software that supports electronic signatures and records• Objective RNA quality measurement with new RNA Integrity Score (RIS)

Sample QC and Purity Analysis

An evaluation of sample quality and quantity is crucial to assessing whether a sample is suitable for archiving and/or demanding downstream assays such as real-time PCR, microarrays or next generation sequencing. We have a range of solutions to fit your needs.

The BioDrop provides a new generation of micro-volume, UV-Vis spectro-photometers where recalibration is never necessary. Requiring just 0.5 µl of sample (detection limit of 1 ng/µl), the BioDrop saves precious samples and provides reliable results.

The Biodrop

The QIAxpert

The QIAxpert is a small, benchtop, microfluidics based, UV-Vis spectro-photometer that can process 16 samples in less than 90 sec. With a sample input of 2 µl, the QIAxpert has a detection limit of 1.5 ng/µl. The advanced analysis offered by the QIAxpert is capable of spectrally deconvoluting samples to:

• Check turbidity• Differentiate molecules of interest• Detect other absorbing impurities• Quantify DNA, RNA or protein fraction specifically

• Never have to calibrate, in-built sample port has no moving parts offering maintenance free performance • Accurate, affordable low volume spectroscopy• USB connectivity for easy PC connection or data export• Easy to clean sample port• Fast and easy to use

The QIAxcel Advanced

The QIAxcel Advanced offers effortless DNA, RNA, and protein analysis for up to 96 samples at a time.

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• GenDx NGS-Go HLA typing kits• GenDx SBTxcellerator HLA typing kits

Sample Utilisation and Analysis

• QIAGEN real-time PCR assays• QIAGEN assays and pathway specific arrays (SABiosciences)• Integrated DNA Technologies (IDT) PrimeTime assays and probes

• QIAGEN Multiplex PCR and Type-it kits (HRM, SYBR, Probe and Microsatellite)• QIAGEN qBiomarker Somatic Mutation PCR Arrays (SABiosciences)• QIAGEN qBiomarker Copy Number PCR Arrays (SABiosciences)• Illumina Omni Family of Microarrays

• QIAGEN EpiTect Whole Bisulfitome Kit (whole genome amplification of bisulfite converted DNA)• QIAGEN EpiTect HRM PCR Kit• QIAGEN EpiTect Methyl II PCR Assays and Arrays (SABiosciences)• Illumina methylation arrays

• QIAGEN miScript Primer Assays• QIAGEN miScript miRNA PCR Arrays (SABiosciences)

• Illumina whole genome sequencing• Illumina targeted resequencing (custom or catalogued panels)• Illumina RNA Sequencing• Illumina small RNA sequencing, CHIP-Seq and methylation sequencing• Integrated DNA Technologies (IDT) xGen® Lockdown® Probes and Blocking Oligos for target capture

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Whitehead Scientific offers an extensive portfolio of cutting-edge solutions for the downstream interrogation of samples

Gene expression

Genotyping

Epigenetics

miRNA research

Next-generation sequencing

HLA typing

Whitehead Scientific is a proud supplier of:

Whitehead Scientific (Pty) LtdSouth Africa - Cape Town

(tel) +27 (0) 21 944 6460 - (fax) +27 (0) 21 949 5478PO Box 194, Brackenfell, 7561, Cape Town, South Africa

whitesci@whitesci.co.za - www.whitesci.co.za