snp genotyping and animal cell culture techniques

7/31/2019 SNP Genotyping and Animal Cell Culture techniques

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Bhgkrh z~mr~ofi ykre jdhje ~dh ofgkrcm~okf iowhf yo~d ~dh jhaa aofh ~k onhf~og

ydm~ chnom ~ph! mnno~owhz mfn rhjkcchfnm~okfz zdkxan bh xzhn&

Ckz~ jhaa aofhz jmf bh irkyf xzofi NCHC jxa~xrh chnom kr RPCO jxa~xrh chnom

yo~d =0( Gh~ma Bkwofh Zhrxc $GBZ/ mfn mf~obok~ojz jmf bh mnnhn og rh}xorhn&

Ckz~ jhaa aofhz yoaa irky kf jxa~xrh gamzez yo~dkx~ ~dh fhhn gkr zphjoma cm~rothz

h~j& Dkyhwhr! zkch jhaaz! pmr~ojxamra procmr jhaaz! yoaa rh}xorh irky~d kf

zphjoma cm~rothz zxjd mz jkaamihf ~k prkck~h jhaa m~~mjdchf~! nogghrhf~om~okf kr

jhaa irky~d&

Cm~hromaz rh}xorhn

=&NCHC

2&GBZ:&Mf~ocjk~oj mf~obok~oj zkax~okf

Prkjhnxrh

m/ Chnom prhpmrm~okfo& Prhpmrm~okf kg NCHC

M& Mnn pkynhrhn chnoxc ~k =8J ~k :0 J $Rkkc ~hcphrm~xrh/

mx~kjamwhn ym~hr yo~d ihf~ah z~orrofi&$Nk fk~ dhm~/&

B& Rofzh kx~ ofzonh kg pmjeh~ ~k rhckwh ~rmjhz kg pkynhr&

J& Mnn =&9i kg FmDJK: phr ao~rh kg chnoxc&

N&Noax~h ~k nhzorhn wkaxch ym~hr& Z~or xf~oa nozzkawhn&


37/58


38/58

:4

=&NCHC >8ca

2&=0( GBZ 8 ca

:&Mf~obok~oj mf~ocjk~oj zkax~okf 0&8 ca

80 ca

Prkwonofi jxa~xrofi jkfno~okfz

Jhaa aofhz mrh cmof~mofhn m~ :90J ofjxbm~kr m~ 8( JK2&

Gkr dxcmf jhaaz! jkm~ gamzez yo~d =( iham~of&

Prhpmrh =0cA kg jkm~ofi zkax~okf jkcpkzhn kg =( iham~of b noax~ofi yo~d

noz~oaahn ym~hr! gkaakyhn b goa~rm~okf& ^doz oz hggojohf~ ~k jkm~ mbkx~ 8 gamzez&

=& Poph~~h jkm~ofi zkax~okf of~k gamze& Rkje bmje mfn gkr~d ~k hwhfa

noz~robx~h ~dh bk~~kc kg ~dh gamze& Ah~ zo~ of mf ofjxbm~kr gkr =8#:0

cofx~hz&

2& Jkcpah~ha rhckwh jkm~ofi zkax~okf b mzporm~ofi bhgkrh zhhnofi&


39/58

:?

Htphrochf~ B:JDHJEOFI JHAAZ

Jhaaz zdkxan bh jdhjehn cojrkzjkpojmaa nmoa ~k hfzxrh ~dh mrh dhma~d

mfn irkyofi mz htphj~hn& M~~mjdhn jhaaz zdkxan bh cmofa m~~mjdhn ~k ~dh

bk~~kc kg ~dh gamze! rkxfn mfn paxcp kr hakfim~hn of zdmph mfn rhgrmj~ofi aoid~

mrkxfn ~dhor chcbrmfh& Zxzphfzokf jhaaz zdkxan akke rkxfn mfn paxcp mfn

rhgrmj~ofi aoid~ mrkxfn ~dhor chcbrmfh& Zkch zxzphfzokf jhaaz cm jaxcp&

Chnom zdkxan bh pofe#krmfih of jkakr&

Nozjmrn jhaaz og0

Htphrochf~ B>ZXBJXA^XRH KG MNDHRHF^ JHAA AOFHZ

Moc

Mndhrhf~ jhaa aofhz yoaa irky of wo~rk xf~oa ~dh dmwh jkwhrhn ~dh zxrgmjh mrhm

mwmoambah kr ~dh chnoxc oz nhpah~hn kg fx~rohf~z& M~ ~doz pkof~ ~dh jhaa aofhz

zdkxan bh zxb#jxa~xrhn of krnhr ~k prhwhf~ ~dh jxa~xrh nofi& Jhaa pmzzmiofi kr

zpao~~ofi oz m ~hjdfo}xh ~dm~ hfmbahz mf ofnowonxma ~k ehhp jhaaz maowh mfn

irkyofi xfnhr jxa~xrhn jkfno~okfz gkr ht~hfnhn phroknz kg ~och& Jhaaz zdkxanbh pmzzhn ydhf ~dh mrh 48(#?0( jkfgaxhf~& ^k zxbjxa~xrh ~dh jhaaz ~dh fhhn

~k bh brkxid~ of~k zxzphfzokf& ^dh nhirhh kg mndhzokf wmrohz grkc jhaa aofh ~k

jhaa aofh bx~ cmlkro~ kg jmzhz prk~hmzhz! h&i& ~rpzof !mrh xzhn ~k rhahmzh ~dh

jhaaz grkc ~dh gamze! Dkyhwhr! ~doz cm fk~ bh mpprkprom~h gkr zkch ~och ydhrh

htpkzxrh ~k prk~hmzhz oz dmrcgxa kr ydhrh ~dh hf|chz mrh xzhn ~k rhckwh

chcbrmfh cmrehrz+rhjhp~krz kg of~hrhz~& Of ~dhzh jmzhz jhaaz zdkxan bh

brkxid~ of~k zxzphfzokf of~k m zcmaa wkaxch kg chnoxc chjdmfojmaa yo~d ~dh

mon kg jhaa zjrmpphrz&

Cm~hromaz rh}xorhn

NCHC#GBZ chnoxc

PBZ+HN^M zkax~okf

0&08( ^rpzof

H}xopchf~

Phrzkfma prk~hj~owh h}xopchf~

Cojrkbokakiojma zmgh~ jmbofh~

JK2 ofjxbm~kr

Prh#ambhahn gamzez


41/58

>=

Cmrehr phf

Cojrk poph~~hz

Mcpxah rmje

Prkjhnxrh

=& Nozjmrn ~dh chnom grkc ~dh jxa~xrh gamze&

2& Ymzd ~dh jxa~xrh gamze ~yojh yo~d 2ca PBZ+HN^M&

:& Mnn ^rpzof mfn ihf~a zdmeh o~ zk ~dm~ jhaaz ih~ nh~mjdhn&

>& Mnn =ca grhzd chnom ~k nhmj~owm~h ~rpzof

8&^rmfzghr ~k m jhf~rogxih ~xbh

7& Jhf~rogxih m~ =000rpc gkr : cof

9& Nozjmrn zxphrfm~mf~

4& Mnn =ca grhzd chnom mfn ~rmfzghr ~k jxa~xrh gamze&


42/58

>2

Htphrochf~ B8

JKXF^OFI JHAAZ YO^D DHCKJ_^KCH^HR

m& Prhpmrofi dhckj~kch~hr

o& Hfzxrh ~dh dhckj~kch~hr oz jahmf xzofi 90( h~dmfka&

oo& Ckoz~hf ~dh zdkxanhrz kg ~dh dhckj~kch~hr mfn mggot ~dh jkwhrzaop xzofi

ihf~ah prhzzxrh mfn zcmaa jorjxamr ck~okfz& ^dh pdhfkchfkf kg Fhy~kfz rofiz

jmf bh kbzhrwhn ydhf ~dh jkwhrzaop oz jkrrhj~a mggothn! ~dxz ~dh nhp~d kg ~dh

jdmcbhr oz hfzxrhn&

b& Jkxf~ofi

o& Xzofi ~dh Ioazkf poph~~h! nrmy xp zkch jhaa zxzphfzokf jkf~mofofi ~rpmf

baxh& Jmrhgxaa goaa ~dh dmhckj~kch~hr b ihf~a rhz~ofi ~dh hfn kg ~dh Ioazkf

~op m~ ~dh hnih kg ~dh jdmcbhrz& ^meh jmrh fk~ ~k kwhr# goaa ~dh jdmcbhr& Maaky

~dh zmcpah ~k bh nrmyf kx~ kg ~dh poph~~h b jmpoaamr mj~okf! ~dh gaxon zdkxan

rxf ~k ~dh hnihz kg ~dh irkkwhz kfa& Rh#akmn ~dh poph~~h mfn goaa ~dh zhjkfn

jdmcbhr og rh}xorhn&

oo& Gkjxz kf ~dh iron aofhz kg ~dh dhckj~kch~hr xzofi ~dh =0T kblhj~owh kg ~dhcojrkzjkph& Gkjxz kf kfh zh~ kg =7 jkrfhr z}xmrh m~ ~dh jkrfhrz&

ooo& ^meh ~dh mwhrmih kg ~dh fxcbhr kg jhaaz gkxfn m~ ~dh jkrfhrz&

ow& Jhaaz phr ca 3 ~dh mwhrmih jkxf~ phr z}xmrh t ~dh noax~okf gmj~kr t =0>


43/58

>:

Htphrochf~#B7

GRHH\OFI KG JHAA#AOFH

Moc

O~ oz jkcckf prmj~ojh ~k jrhm~h m cmz~hr bmfe jkfzoz~ofi kg 2 ~k 20 womaz kg ~dh

jhaa aofh& ^dhf jrhm~h kfh kr ~yk ykreofi bmfez grkc ~doz yo~d 2 ~k 20 womaz of

hmjd $nhphfnofi kf dky kg~hf ~dh jhaaz yoaa bh rh}xorhn/& Ydhf ~dh ykreofi

bmfe oz xzhn xp! m fhy ykreofi bmfe jmf bh jxa~xrhn mfn jrhm~hn grkc kfh

woma kg ~dh kroiofma cmz~hr bmfe& Og pkzzobah! ehhp ~dh cmz~hr mfn ykreofi bmfe

of zhpmrm~h ao}xon fo~rkihf z~krmih ~mfez&

^doz yoaa hfzxrh kx maymz dmwh m z~kje kg jhaaz grkc m akyhr pmzzmih fxcbhr

mfn o~ yoaa mazk fk~ bh fhjhzzmr ~k ehhp pxrjdmzofi ~dh jhaa aofh&

Cm~hromaz

=& = ca # 2 ca jrkwoma

2& Jhaa jxa~xrh chnoxc yo~d 20( GBZ $Gh~ma bkwofh zhrxc/ mfn fhjhzzmr

zxppahchf~z

:& NCZK $Noch~da zxagktonh/! doid pxro~! z~hroah! gkr jhaa jxa~xrh

>& Prhpmrh grhh|ofi chnoxc< ~k jhaa jxa~xrh chnoxc! mnn 8#=0( $w+w/

NCZK&

Prkjhnxrh

=& Zpao~ ~dh jhaaz& ^meh =ca ~k jrkwoma&2& Jhf~rogxih m~ 2800rpc gkr : cofx~hz m~ > J&

:& Nozjmrn ~dh zxphrfm~mf~ mfn mnn 980xa grhh|ofi cot~xrh $?ca GBZ % =ca

NCZK/&

>& Z~krh m~ #20 J gkr = nm! ~dhf of #90 J $2#> nmz/ mfn ~dhf of ao}xon F2&


44/58

>>

Prhjmx~okfz

^doz z~hp cxz~ bh nkfh mz zkkf mz ~dh jhaaz mrh of grhh|ofi chnom& NCZK mfn

zkch k~dhr jrkprk~hj~mf~z mrh ~ktoj ~k jhaaz mfn zk zdkxan fk~ bh htpkzhn ~k

~dh jhaaz m~ rkkc ~hcphrm~xrh gkr mf akfihr ~dmf fhjhzzmr& ^dmyofi kg ~dh

womaz mfn pamjofi kg ~dh jhaa zxzphfzokf bmje of~k jxa~xrh chnom zdkxan mazk bh

nkfh whr }xojea gkr ~dh zmch rhmzkfz&


45/58

>8

Htphrochf~ B9

RHWOWMA KG JHAA AOFHZ

MocCmf jxa~xrhz kb~mofhn grkc m jxa~xrh jkaahj~okf yoaa mrrowh grk|hf mfn of krnhr

~k xzh ~dhc! ~dh jhaaz cxz~ bh ~dmyhn mfn px~ of~k jxa~xrh& O~ oz wo~ma ~k ~dmy

jhaaz jkrrhj~a of krnhr ~k cmof~mof ~dh womboao~ kg ~dh jxa~xrh mfn hfmbah ~dh

jxa~xrh ~k rhjkwhr ckrh }xojea& Zkch jrkprk~hj~mf~z zxjd mz NCZK mrh ~ktoj

mbkwh > J& ^dhrhgkrh o~ oz hzzhf~oma ~dm~ ~dh jxa~xrhz bh ~dmyhn }xojea noax~hn

of jxa~xrh chnoxc ~k cofoco|h ~dh ~ktoj hgghj~z&

Cm~hromaz rh}xorhn

Chnom# prh#ymrchn ~k ~dh mpprkprom~h ~hcphrm~xrh&

H}xopchf~ rh}xorhn

Phrzkfma prk~hj~owh h}xopchf~ $z~hroah iakwhz! ambkrm~kr jkm~/

Ym~hrbm~d zh~ ~k mpprkprom~h ~hcphrm~xrh

Cojrkbokakiojma zmgh~ jmbofh~ m~ mpprkprom~h jkf~mofchf~ ahwha JK2 ofjxbm~kr

Prh ambhahn gamzez

Cmrehr phf

Cojrkpoph~~hz

Mcpxah rmje

^ozzxh

Prkjhnxrh

=& ^meh ~dh jhaaz grkc #40 J& ^dmy o~ m~ :9 J

2& Jhf~rogxih m~ 2000 rpc gkr 8 cof

:& Nozjmrn ~dh zxphrfm~mf~&


46/58

>7

>& ^rmfzghr ~k =8 ca ~xbh& Mnn 8ca 20( NCHC

8& Jhf~rogxih m~ 2000 rpc gkr 8 cof mfn nozjmrn ~dh zxphrfm~mf~&

7& ^meh 8 ca 20 ( NCHC of jxa~xrh gamze& ^rmfzghr phaah~ ~k o~

9& Z~mr~ cmof~mofofi ~dh jhaa aofh


47/58

>9

Htphrochf~ B4

JHAA ZHHNOFI GKR NFM+RFM OZKAM^OKF

Prkjhnxrh

=& Nozjmrn htoz~ofi chnom

2& Mnn 2 ca PBZ#HN^M mfn ymzd ~yojh

:& Mnn ~rpzof mfn ihf~a zyora

>& Mnn = ca chnom

8&^rmfzghr ~k Jhf~rogxih ~xbh

7& Jhf~rogxih m~ =000rpc gkr : cof

9& Nozjmrn zxphrfm~mf~4& Mnn =ca grhzd chnom

?& Noax~h o~ =0 gkan yo~d chnom&

=0& ^meh =0xa mfn jkxf~ ~dh fxcbhr kg jhaaz xzofi dmhckj~kch~hr&

==& Yh rh}xorh : t =08 jhaaz of m jxa~xrh pam~h& Zk! yo~d ~dh dhap kg

W=F=3W2F2

Yh jmajxam~h ~dh wkaxch kg chnom ~k bh mnnhn ~k hmjd jxa~xrh pam~h&

=2& Mnn U=000#$wkaxch kg jhaa mnnhn/ S chnom ~k hmjd jxa~xrh pam~h

=:& Ofjxbm~h m~ :9J


48/58


49/58

>?

Htphrochf~ B=0

NFM OZKAM^OKF GRKC JXA^XRHN JHAA AOFH

$]OMIHFNFMozkam~okfeo~/

=& Jhaaz zxzphfnhn of NCHC z~krhn m~ #20 J

2& Rhckwh ~dh zxphrfm~mf~ jkcpah~ha mfn nozjmrn

:& Mnn 20xa PBZ ~k rhzxzphfn ~dh phaah~

>& Mnn 20xa prk~hofmzh E

8& Mnn 20xa bxgghr MA $azoz bxgghr/ mfn cot b pxazh wkr~htofi gkr =8z

7& Ofjxbm~h m~ 87 J gkr =0 cof

9& Mnn 200xa jdoaahn majkdka $70#=00(/ ' cot b pxazh wkr~htofi gkr =8z

4&^rmfzghr ~k cofo zpof jkaxcf ' jhf~rogxih m~ 4000rpc! 2:#28 J gkr 2

cof

?& Nozjmrn ~dh goa~rm~h

=0& Mnn Bxgghr MY=$ymzd bxgghr/ 800 xa ' jhf~rogxih m~ 4000rpc! 2:

28 J gkr 2 cofx~hz

==& Nozjmrn ~dh goa~rm~h

=2& Mnn 800xa Bxgghr MY2$ymzd bxgghr/

=:& Jhf~rogxih m~ =>000 rpc m~ 28 J gkr : cof

=>& Mnn 200xa Bxgghr MH$hax~okf bxgghr/

=8& Ofjxbm~h m~ rkkc ~hcphrm~xrh gkr 8 cof

=7& Jhf~rogxih m~ 4000 rpc m~ 28 J gkr 2 cof

=9& Z~krh m~ #20 J


50/58

80

Htphrochf~ B==

]XMF^OGOJM^OKF XZOFI FMFKNRKP

Moc

Pxro~ kg NFM zmcpah jmf mazk bh jmajxam~hn bmzhn xpkf o~z mbzkrbmfjh kg

aoid~& M pxrh zmcpah kg NFM dmz m 270fc+240fc rm~ok kg =&4& Rm~okz nhwom~ofi

grkc ~doz xzxmaa ofnojm~h jkf~mcofm~okf kg ~dh zmcpah yo~d prk~hofz! krimfoj

zkawhf~z kr RFM kr jkxan ofnojm~h nhirmnm~okf kg ~dh NFM zmcpah&

Cm~hromaz Rh}xorhn

=&Fmfknrkp

Prkjhnxrh

=& Jahmf ~dh zmcpah akmnofi pkof~ yo~d z~hroah ym~hr ~k ofo~omao|h ~dh

h}xopchf~

2& Kphf ~dh bxfnahn zkg~ymrh

:& Mnn =xa kg fxjahmzh grhh ym~hr zkax~okf of ydojd NFM oz nozzkawhn mfn

jmajxam~h Bamfe

>& Fky jahmf ~dh akmnofi pkof~ mfn akmn zxbzh}xhf~ zmcpahz mfn chmzxrh

}xmf~ogojm~okf&

8& Rhjkrn ~dh wmaxhz kg NFM jkfjhf~rm~okf$fi+xa/ mfn M270+M240


51/58

8=

Htphrochf~ B=2

RFM OZKAM^OKF GRKC JXA^XRHN JHAA AOFHZ $^RO\KA

CH^DKN/

Prkjhnxrh

=& Ymzd jhaaz yo~d =T PBZ&

2& Mnn =ca ^RO|ka phr yhaa&

:& Ofjxbm~h m~ rkkc ~hcphrm~xrh gkr : cofx~hz

>& Mnn 200xa JDJa:

8& Pxazh wkr~ht gkr =8 zhjkfnz mfn ofjxbm~h m~ rkkc ~hcphrm~xrh gkr :

cofx~hz&7& Jhf~rogxih gkr =8 cofx~hz m~ 4000 rpc m~ > J&

9& Poph~~h ym~hr pdmzh of~k m fhy hpphfnkrg ~xbh&

4& Mnn 800xa ozkprkpmfka phr ca kg ~ro|ka

?& Ofjxbm~h m~ #20 J gkr :0cofx~hz&

=0&Jhf~rogxih gkr =0 cofx~hz m~ =>000rpc m~ > J

==&Ymzd phaah~ yo~d 800xa 90( h~dmfka&

=2& Jhf~rogxih gkr 8 cof m~ =>000rpc m~ rkkc ~hcphrm~xrh&

=:& Mor nr ~dh phaah~ $20 cof/

=>& Rhzxzphfn of :0#80xa fxjahmzh#grhh ym~hr&

=8&Ehhp m~ 87 J gkr =0 cofx~hz

=7&Z~krh m~ #40J&


52/58

82

Htphrochf~# B=:

MZZHZZCHF^ KG ]XMAO^_ MFN ]XMF^O^_ KG RFM&

m/ ]xmao~ kg RFM jmf bh jdhjehn b mimrkzh hahj~rkpdkrhzoz gkaakyhn b

h~donoxc brkconh z~mofofi& 2a kg RFM oz rxf kf =&2( mimrkzh iha mfn

pdk~kirmpdhn&

^dh prhzhfjh kg jrozp bmfnz jkrrhzpkfnofi ~k 24 Z rRFM mfn =4 Z rRFM

ofnojm~hz ~dh }xmao~ kg ~dh RFM ozkam~hn&

b/ RFM jkfjhf~rm~okf jmf bh chmzxrhn xzofi FmfkNrkp^C

zphj~rkpdk~kch~hr&

M270+M240 rm~ok gkr RFM zdkxan bh =&4#2& M270+M2:0 rm~ok zdkxan bh 2#2&2

Prkjhnxrh

=& Jahmf ~dh zmcpah akmnofi pkof~ yo~d z~hroah ym~hr&

2& Kphf ~dh bxfnahn zkg~ymrh

:& Mnn =xa kg fxjahmzh grhh ym~hr zkax~okf of ydojd RFM oz nozzkawhn mfn

jmajxam~h Bamfe

>& Fky jahmf ~dh akmnofi pkof~ mfn akmn zxbzh}xhf~ zmcpahz mfn chmzxrh

}xmf~ogojm~okf&

8& Rhjkrn ~dh wmaxhz kg RFM jkfjhf~rm~okf M270+M240 mfn M270+M2:0&


53/58

8:

Htphrochf~ B=>

jNFM PRHPMRM^OKF

Prkjhnxrh

=& Prhpmrh m cot~xrh of mf hpphfnkrg ~xbh yo~d ~dh gkaakyofi jkfz~o~xhf~z

=0 t R^ bxgghr 2 xa

28T nF^P 0&4 xa

=0 T rmfnkc prochr 2 xa

Rhwhrzh ~rmfzjrop~mzh = xa

Fxjahmzh Grhh Ym~hr >&2 xa

=0xa

2& Prhpmrh = xi kg RFM of =0 xa

:& Cot =0xa kg RFM mfn =0 xa kg rhmj~okf cot&

>& Pamjh ~dh ~xbh of m ~dhrcma jjahr mfn rxf ~dh gkaakyofi prkirmc

28 kJ =0 cof

:9 kJ =20 cof

48 kJ 8 cof

> kJ ofgofo~

]XMAO^_ JKF^RKA ^HZ^ GKR jNFM

^doz ~hz~ ymz bhofi phrgkrchn b PJR mcpaogojm~okf kg#Mj~of&

Rhmj~okf cot~xrh ymz prhpmrhn yo~d gkaakyofi jkcpkfhf~z

jNFM # =A


54/58

8>

=0TR^ bxgghr # =A P&^&K

2&8cCnF^P # =A

20 xC gkrymrn prochr # 0&0?xa

20 xC rhwhrzh prochr # 0&0?xa

^m} pkachrmzh # 0&2xa

D2K # 7&72xa

=0xa

PJR jkfno~okfz

?8`J : cof

?8J :0 zhj

87&9J =8 zhj

92J :0 zhj

92J =0 cof

>J

Gkaakyofi PJR!~dh prknxj~ ymz rxf of =( iha mfn mfmazhn gkr bmfnz

jkrrhzpkfnofi ~k B#mj~of&


55/58

88

Htphrochf~ B=8RHMA#^OCH PJR

Moc

^doz ~hjdfo}xh oz xzhn ~k mcpaog mfn zocxa~mfhkxza }xmf~og m

~mrih~hn NFM ckahjxah& Dhrh ~dh mcpaogohn NFM oz nh~hj~hn mz ~dh rhmj~okf

prkirhzzhz of rhma ~och&^yk jkcckf ch~dknz gkr nh~hj~okf kg prknxj~z of rhma#

~och PJR mrh< $=/ fkf#zphjogoj gaxkrhzjhf~ nhz ~dm~ of~hrjmam~h yo~d mf

nkxbah#z~rmfnhn NFM! mfn $2/ zh}xhfjh#zphjogoj NFM prkbhz jkfzoz~ofikg kaoikfxjahk~onhz ~dm~ mrh ambhahn yo~d m gaxkrhzjhf~ rhpkr~hr ydojd phrco~z

nh~hj~okf kfa mg~hr dbrono|m~okf kg ~dh prkbh yo~d o~z jkcpahchf~mr NFM

~mrih~&


56/58

87

^dhrh mrh bmzojmaa ~yk ch~dknz kg mfma|ofi ~dh nm~m grkc m rhma ~och PJR#

mbzkax~h }xmf~ogojm~okf mfn rham~owh }xmf~ogojm~okf& Rham~owh }xmf~ogojm~okf kr

jkcpmrm~owh }xmf~ogojm~okf chmzxrhz ~dh rham~owh jdmfih of cRFM htprhzzokf

ahwhaz& O~ nh~hrcofhz ~dh jdmfihz of z~hmn z~m~h cRFM ahwhaz kg m ihfh mjrkzz

cxa~opah zmcpahz mfn htprhzzhz o~ rham~owh ~k ~dh ahwhaz kg mfk~dhr RFM& Rham~owh

jkfjhf~rm~okfz kg NFM prhzhf~ nxrofi ~dh htpkfhf~oma pdmzh kg ~dh rhmj~okf mrh

nh~hrcofhn b pak~~ofi gaxkrhzjhfjh mimofz~ jjah fxcbhr kf m akimro~dcoj

zjmah $zk ~dm~ mf htpkfhf~omaa#ofjrhmzofi }xmf~o~ yoaa zdky mz m z~rmoid~ aofh/&

M ~drhzdkan gkr nh~hj~okf kg gaxkrhzjhfjh mbkwh bmjeirkxfn oz nh~hrcofhn& ^dh

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snp genotyping and animal cell culture techniques

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