Actoprobe Scanning Laser Confocal Microscope

SLM Overview

Designed using top class off-the-shelf components Fully automated Fast Imaging capabilities:4 fp/sec to 30 fp/sec Optical sectioning available up to 100 µm Field of scan up to 280 µms Suitable for wavelengths from 300 to 700 nm Compatible with most AFMs Compatible with upright and inverted microscope configurations

Dimensions

33 inches

20 inches16 inches

Enclosure

Enclosure

System offers very durable and sturdy

enclosure to protect the microscope from airborne noise and

dust

ComponentsScanner and Confocal optics:Galvano Scanners and optical fiber optics

Color Camera

Objectives: Can hold up to 5 objectives

Sample Holder Stage

Lasers and fiber launcher: Accommodate up to 2 lasers

Detector: Compatible with PMT, APD or Silicon detectors

White light source

Confocal System

1kHz or 8kHz Scanners 4 fp/sec to 30fp/sec acquisition rate at 512X512 pixel resolution Confocal aperture diameters up to 1.8 µm Fiber coupled: Suitable for wavelengths from 300 to 700 nm Suitable for objectives from 10X to 100X Suitable for PMT, APD or Silicon detectors Field of View up to 280X280 µm Lateral resolution up to 270 nm and axial resolution up to 370 nm

Sample Holder Stage

Crystal Holder (New Port) Specially Designed Crystal Holder adapter Tip-Tilt-Rotation stage: 5 degrees tip, tilt and rotation Z Piezo Stage: 100 µm travel range close loop with .2 nm resolution for optical sectioning X-Y Stage: 25 mm travel range close loop with 7 nm resolution Focus Block: 2 inches travel range in Z with 1 µm accuracy Automated stage (X-Y-Z) controlled through JoyStick

NLO Crystal

Laser - Fiber Launcher

ND filter: Each laser has an independent intensity control Lasers: 2 to 3 lasers can be used (RGB); current lasers are 100 mW blue and green Signal optimization through chopper Fiber launcher: 2 to 3 fibers can be launched; current configuration- 2 SM fibers (blue and green)

Software: Data Acquisition GUI

Sample Holder Stage GUI

Camera GUI

c Data Analysis GUI

Quick Data Plotter GUI

Optical sectioning of a laser burn on mirror surface

Camera Image

3D Image constructed from individual slices

Slice at the top surface

Slice at 1.5 µm depthSlice at 1 µm depth

Slice at 2.5 µm depthSlice at 2 µm depth

Slice at 500nm depth

Optical sectioning of a laser burn on LBO crystal surface

Camera Image

3D Image constructed from individual slices

Slice at the top surface of pattern Slice at 4 µm depth

Slice at 2 µm depth Slice at 6 µm depth

Optical sectioning of a laser burn on LBO crystal surface

Camera Image

3D Image constructed from individual slices

Slice at the top surface of pattern Slice at 600 nm depth

Slice at 400 nm depth Slice at 800 nm depth