Slide PreparationSlide Preparation

D. Crowley, 2007D. Crowley, 2007

Slide PreparationSlide Preparation

To be able to prepare a microscope slideTo be able to prepare a microscope slide

Tuesday, April 18, 2023

Microscope CrosswordMicroscope Crossword

Have a go at the microscope crossword - you have five minutes…

Down: -

1. Lens that you look down

2. What the slide is placed on

3. A wheel to make the image clear

Across: -

4. Lens closest to the slide

5. The specimen is placed on this

6. You need this to see something

Have a go at the microscope crossword - you have five minutes…

Down: -

1. Lens that you look down

2. What the slide is placed on

3. A wheel to make the image clear

Across: -

4. Lens closest to the slide

5. The specimen is placed on this

6. You need this to see something

EyepieceStageFocusing

ObjectiveSlideLight

MicroscopesMicroscopes

Set up a microscope to view an animal cell - remember the steps to take

Place the smallest objective lens (the smallest lens) over the hole in the stage Turn the coarse focusing wheel to make the gap between the stage and the

objective lens as small as possible Place a slide on the stage, and secure with the clips Adjust the light source so light goes up through the stage Turn the coarse focusing wheel slowing, until your specimen is in focus To see your specimen in more detail, turn the next largest objective lens over your

specimen Use the fine focusing wheel to get your image into focus again - do not use the

coarse focusing wheel - this can break your slide!

Set up a microscope to view an animal cell - remember the steps to take

Place the smallest objective lens (the smallest lens) over the hole in the stage Turn the coarse focusing wheel to make the gap between the stage and the

objective lens as small as possible Place a slide on the stage, and secure with the clips Adjust the light source so light goes up through the stage Turn the coarse focusing wheel slowing, until your specimen is in focus To see your specimen in more detail, turn the next largest objective lens over your

specimen Use the fine focusing wheel to get your image into focus again - do not use the

coarse focusing wheel - this can break your slide!

Preparing SpecimensPreparing Specimens

A specimen is the object we look at under a microscope

The specimen needs to be thin, so light can pass through it

We use a coverslip between the slide and specimen which helps flatten it out. It also helps keep the specimen in place, and stops it drying out

When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:

Magnification = magnification eyepiece lens x magnification objective lens

A specimen is the object we look at under a microscope

The specimen needs to be thin, so light can pass through it

We use a coverslip between the slide and specimen which helps flatten it out. It also helps keep the specimen in place, and stops it drying out

When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:

Magnification = magnification eyepiece lens x magnification objective lens

Preparing OnionsPreparing Onions

You are going to prepare a slide containing a specimen of onion cells

Follow the slide preparation steps, then draw what you see (using A4 plain paper and in pencil). Also write down your magnification

Magnification = magnification eyepiece lens x magnification objective lens

You are going to prepare a slide containing a specimen of onion cells

Follow the slide preparation steps, then draw what you see (using A4 plain paper and in pencil). Also write down your magnification

Magnification = magnification eyepiece lens x magnification objective lens

Place a drop of water on the centre of a slide

(this may contain a stain to make the

specimen more clear, e.g. iodine)

Cut some onion, then using forceps, peel off

the inside layer of a piece of onion

Place your onion skin onto the water droplet

on your slide

Using a mounted needle, lower a

coverslip onto the onion skin. Do this

slowly and carefully, to avoid trapping air

bubbles!

MagnificationMagnification

When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:

Magnification = magnification eyepiece lens x magnification objective lens

E.g. eyepiece lens = 10xobjective lens = 60x

Magnification = 10 x 60

= 600x magnification

When you have prepared your specimen you can look at it under the microscope. It is vital you know what magnification you are using: this is easy to work out - it is the eyepiece lens x the objective lens:

Magnification = magnification eyepiece lens x magnification objective lens

E.g. eyepiece lens = 10xobjective lens = 60x

Magnification = 10 x 60

= 600x magnification