sistemas sensorias prof. m.d. ph.d. fayez bahmad jr
DESCRIPTION
Sistemas Sensorias Prof. M.D. Ph.D. Fayez Bahmad Jr. Mestrando Leonardo Costa Pereira. Introdução. Introdução. Crescimento Aleatório Carcinoma (DCC) + Netrin 1. Objetivo. - PowerPoint PPT PresentationTRANSCRIPT
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Sistemas SensoriasProf. M.D. Ph.D. Fayez Bahmad Jr
Mestrando Leonardo Costa Pereira
![Page 2: Sistemas Sensorias Prof. M.D. Ph.D. Fayez Bahmad Jr](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56813445550346895d9b2ffb/html5/thumbnails/2.jpg)
![Page 3: Sistemas Sensorias Prof. M.D. Ph.D. Fayez Bahmad Jr](https://reader036.vdocuments.mx/reader036/viewer/2022062308/56813445550346895d9b2ffb/html5/thumbnails/3.jpg)
Introdução
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Introdução
Crescimento Aleatório
Carcinoma (DCC) + Netrin 1
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Objetivo
Determinar os efeitos de Netrin 1, guiados pelo gene DCC, quanto a orientação e crescimento do axônio do neurônio situado no Gânglio Espiral, em uma orelha adulta.
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Material e Métodos O presente estudo utilizou-se de tecidos de
camundongos e os gânglios acústicos de galinhas.
Os Prime’s (By PCR) 5!AGTSTGTCTCAACTGCCGCC3! (netrin-1 sense)
5!TACACGGAGATGATGTTCACGG3! (netrin-1 antisense)
5!AGTGCCTCTCATTCAGGTCAGG3! (DCC sense)
5!TCACAGACTGAGTTCTTCCTGC3! (DCC antisense)
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Material e Métodos
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Material e Métodos• As células do gânglio
coclear foram micro dissecadas.
• Trituradas e fixadas em laminas histológicas
• Fixação do gene DCC após 48h
• 120h de cultivo dos neurônios
• 2 grupos (Controle X Netrin 1 + Gene DCC.
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Resultados
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Resultados
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Discussão
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Conclusão O Netrin 1, pode promover uma neurite
orientada dos axônios do Gânglio Espiral, em modelo in vitro.