signaling pathways produced by combining dsrna with paclitaxal to treat ovarian cancer
DESCRIPTION
Signaling Pathways Produced By Combining DsRNA with Paclitaxal to treat Ovarian Cancer. Switu Patel. Ovarian Cancer. Fifth in, malignant deaths of US women Cause unknown Common Therapy: surgery + chemotherapy. Dual treatments. - PowerPoint PPT PresentationTRANSCRIPT
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Signaling Pathways Signaling Pathways Produced By Combining Produced By Combining DsRNA with Paclitaxal to DsRNA with Paclitaxal to treat Ovarian Cancertreat Ovarian Cancer
Switu Patel
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Ovarian CancerOvarian CancerFifth in, malignant deaths of US
womenCause unknownCommon Therapy: surgery +
chemotherapy
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Dual treatmentsDual treatmentsIn Van et al. they combined Double
stranded RNA (DsRNA) with therapeutic agents like paclitaxel and carboplatin to get a significant decrease in cell viability
DsRNA is known to stop the immunosuppression signals caused by ovarian tumor cells and activate four innate immune receptors
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Cell response to dsRNACell response to dsRNA
When stimulated within a cell, it activates four receptors.
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Cell response to dsRNACell response to dsRNA
DsRNA activates Protein Kinase and gives it a pro-apoptotic or cell killing response
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Cell response to dsRNACell response to dsRNA
Which then phosphorylates EIF2S which stops translation
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Cell response to dsRNACell response to dsRNA
Apoptosis
PKR also activates FADD which further activates caspase 8
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Cell response to dsRNACell response to dsRNA
ApoptosisOther pathways that influence apoptosis
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Individual vs. Combined Individual vs. Combined treatmentstreatments
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Proposal questionProposal questionWhat essential pathways related to
apoptosis are activated by dsRNA + paclitaxel? ◦These pathways will serve as biomarkers
for individual patients
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ExperimentExperimentApoptotic PCR ArrayFor:
◦Untreated◦Treated: dsRNA, paclitaxel, dsRNA +
paclitaxel
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Hypothetical ResultsHypothetical Results
Caspase 3
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ResultsResultsWe expect that the samples treated
with combined therapeutic agents will have a higher levels of apoptotic pathway molecules or lower CT values, than the individual treated samples.
However, it may cause down regulation of anti-apoptotic proteins or cause up regulation of pro-apoptotic or cell death proteins.