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Pyropia spheroplasts (PS) as new feed ingredient for culturing Japanese sea cucumber (Apostochopus japonicus)M. ShahabuddinStudent ID:513D3S2

IntroductionCommercially important species in Japan

North West Pacific 35o N to 44o N of Far East Russia FederationOver-exploitation

China

Russia

Japanese seacucumber in East AsiaLanding of Japanese Sea cucumber

Seed production Commercial aquaculture Small scale aquaculture

Japan

Average 5.2 Millions/year

To investigate the effects of dietary inclusion of Pyropia spheroplasts on growth and body composition of Japanese sea cucumber

Aim of the study Optimum nutrition requirements,low cost balanced dietMicroalgae and powdered macroalgae with are using as main components of formulated diet

Materials and methods

A. japonicusExperiment

Collection from nature Acclimatization-2 weeksTemp.-15 oC Water exchange Aeration Photoperiod16:8 D:L Re-circulatingtanks Dry sinking pellets

6

Layout of re-circulating systems

Exp. Tank=Experimental tank, SP= Submersible pump, PSk= Protein skimmer, MF= Mechanical filter, BF=Biological filter, MP=Main circular pamp, UV=Ultra violet, Chiller In and Out, Aeation pump and Thermal controller system

Experimental set-upCentrifuge and keep in refrigerator

Ingredients1%PS Diet3%PS Diet5%PS DietControlDietSoybean meal50505050Rice powder15151515Rice bran15151515White Fish meal97510Trace mineral premix3333Vitamin premix2222CMC4444Chromic Oxide1111Pyropia spheroplasts1350Protein35.735.534.334.7Lipid3.13.13.13.2Ash9.79.010.19.9

FecesFeedConsuming feed bytentacles Eating and discharging

Consumed almost all feeds

Searching for food Searching for foodMoving towards to the food

No competition for foodLiving togetherHealthy with sharp spikes

ResultsWater quality parametersDO and pHNH3-N and NO2-NNitrosomones bac. NH4 NO2Nitrobacter bac. NO2 NO3

Phosphorus and NO3-NMean weight (g/week)

Total weight gain of sea cucumbers during the experiment (g)

Control

1%PS3%PS5%PS

Mean weight gain of sea cucumbers during the experiment (g)

1%PS3%PS5%PS

Net yield of sea cucumbers during the experiment (kg/m3/42days)

1%PS3%PS5%PS

Specific growth rate (%) between treatments

Feed conversion efficiency (%) by sea cucumbersduring the experiment

Muscle Protein gain in different treatments

Protein Efficiency ratio (PER) between treatments

Protein Productive Value (PPV%)

Exponential growth was observed among the PS dietsDiscussionTotal weight gain, Mean weight gain and Net yield was significantly higher in 5% PS dietGradual increase of growth was observed in all the treatments

Highest SGRs and Feed conversion efficiency was observed in higher percentage of PS than control

Biochemical analysis also showed that PS diet is better than non PS dietIncreasing level of PS showed better performance 5% PS can be added in the formulated diet of Japanese sea cucumber

Thank you