session 7: development of a female q-fly lure
TRANSCRIPT
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biosecurity built on science
Development of a female Qfly lure
Andrew Finlay, Summerfruit Australia&
Paul CunninghamResearch Leader, AgriBio, DEDJTR, VIC.
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biosecurity built on science
Female Qfly LuresFeeding
Egg laying
Bactrocera tryoni
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biosecurity built on science
Phase 1: Proof of concept
Blends containing fruit ripening volatiles attract female Qfly
Proof of concept
11V
• analytical chemistry• blend formulation• electrophysiology• cage trials
A SYNTHETIC FRUIT ODOUR TOATTRACT MATED FEMALE FLIES
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biosecurity built on science
Phase 2: Formulations & Field studiesFinding the right mix
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biosecurity built on science
Phase 2: Formulations & Field studiesFinding the right mix FEMALE PHEROMONES
Day 12 mated
Day 3 virgin
Deterrent?
• Solvent extraction• Rectal gland extracts• Insect emissions
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biosecurity built on science
Phase 2: Formulation & Field studiesFinding the right mix YEASTS Cryptococcus flavescens
Aureobasidium pullulans
Rhodotorula sp (2)
Hannaella sp (3)
Pichia fermentans
Sydowia eucalypti
C. flavescens
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biosecurity built on science
Phase 2: Formulation & Field studiesFinding the right mix Deterrents
• Females avoid larval infested fruit• Acetoin emissions
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biosecurity built on science
Phase 2: New technologies
Applying calcium imaging of the antennal lobe to insect lure development
Volatile-evoked responsesin the Qfly antennal lobe
Qfly brain
Improved methods for attractant formulation across insect pests
• blend synergy• volatile detection• valence
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biosecurity built on science
Fieldwork 2016/17Field trials:
2. Mated vs unmated capture rates• feeding vs egg laying lures
1. Trial lure formulations • x 4 formulations• New dispensers
Tatura (VIC)
Stanthorpe (QLD)
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biosecurity built on science
Benefits of female lure research
Commercial fruit growers / consultants Monitoring / surveillance Reducing Qfly damage Lowering within / between season populations Reducing urban Qfly populations Improved market access Reduction in production losses
Sterile Insect Technique Assessment of success Lowering populations
Scientists Olfactory research Developing new technologies for pest management
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biosecurity built on science
Thank you
Project TeamJane Royer (QDAF)Pawel Sadowski (QUT/CARF)Mikael Carlsson (Stockholm University, AL imaging)Tony Clarke (QUT)
Tommaso Villa (female pheromones)Owen Webb (acetoin emissions)Alex Piper (yeast analysis)
Summerfruit AustraliaAndrew Finlay (Chairman)Rowan Berecry