PowerPoint Presentation

SESSION 2: MORE ACCURATE AND TIMELY DIAGNOSTICS TO BETTER INFORM BIOSECURITY DECISIONS

Rohan Kimber

Plant Biosecurity Cooperative Research CentreProject 2014Part A: New tools for field grains surveillanceJenny Davidson: Senior Plant Pathologist (SARDI)Rohan Kimber: Plant Pathologist (SARDI)Greg Baker: Entomologist (SARDI)Helen De Graaf: Entomologist (SARDI)Les Zeller: Engineer (USQ)Paul Kamel: Engineer (USQ)

New opportunities for pest & disease surveillance using new technologies & sensing systemsBroad scale surveillance (coordinated network)Detect & report endemic or exotic incursions of fungal spores / insects in the field to the decision maker or grower community Local (on farm) outbreaks Regional threats (e.g. rusts) National interests (biosecurity)

Project 2014 New tools for field grains surveillance and diagnostics of high priority exotic pestsNew technology & opportunities

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New technology & opportunitiesTechnologies implementedSpecies specific detection qPCR, Pheromone lures (stacking)Sensors - optical, impact & digital to confirm targetSmart capture GPS, environment or diurnal triggersWireless / telemetry networked grid & transmissionSystems being developed / evaluated Smart insect traps (moths) in field detection and imagerySuction insect traps (aphids) localized monitoringLab qPCR diagnostics (mixed populations)Smart traps (pathogens) mobile & fixed systemsLab qPCR diagnostics & toward real-time sensor detectionField evaluation, technology adaptation, collaborationDAFF/USQ & SARDI Qld and SA test sitesRothamsted & Burkard Manufacturing Co. UKResearch & opportunities Surveillance and New technologiesNew technology & platforms pest & disease surveillance PBCRC Project 2014BCA

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Next generation trapping (2015 - )High sampling efficiency & automation systemsSmart capture GPS, meteorological triggers, data analyticsMore compatible or adaptable to rapid down-stream diagnosticsLab-based qPCR species specific primers for accurate IDToward real-time detection (in-field) eg. LAMP, biosensors, fiber optic sensing

Fixed locationLow sampling rate = 10 l/minAdhesive tape (impact sampling)Retrospective analysis DNA probesSeveral endemic pathogens to pulse & cerealsSARDI & collaborators SA, Vic, NSW, WA

Current status Trapping

Research & opportunities Airborne spore surveillance

Systems developed/being evaluated by PBCRC project 2014Collaborative effortInnovation - Prof Jon West (Rothamsted Research)Engineering Stuart Wili, Burkard Manufacturing Co (UK) Jet Sampler Les Zeller, Paul Kamel, Bruen Smith, USQ NCEA SMARTs technology

Smart Spore Traps - meteorological triggers eg. Temp, RH, diurnal, wind speed/direction- Impactor type (capture on adhesive type) CRC30032- Miniature Virtual Impactor (direct capture to tube)

Jet Spore Sampler - Virtual impaction system, high sampling rate, 20-tube carrousel system- Mobile trapping - Smart capture (GPS)

BurkardBurkardUSQ

Burkard

New technology & platforms Airborne spore surveillance PBCRC Project 2014

New technology & opportunities Jet Spore SamplerJet Spore Sampler Burkard Manufacturing Co. Prototype 1 delivered to SARDI Mobile Jet Spore SamplerSMART technology adaptation being finalised at USQ GPS and climate data (temp, wind direction, RH)

Prototype 2 delivered to Rothamsted Research (UK) Prof Jon West Jet Spore SamplerFixed (static) location air sampling. For evaluation in Rothamsted wind tunnel optimise operational function and collection efficiency That version will not be a mobile device.Visits supported by PBCRC Small Initiative Projects SI20004 & SI20060

New technology & opportunities Jet Spore SamplerJet Spore Sampler Evaluation at Rothamsted Research (July 2016) with Prof Jon WestPrototype 2 determine collection efficiency in comparison to Burkard Volumetric TrapLycopodium clavatum (used in aerobiology studies) approx 35um diamGiant puffball spores (Lycoperdon giganteum) approx. 3.5um diam

Standard vs Multi-vial vs Jet Spore Samplers Rothamsted Wind Tunnel July 2016Lycopodium clavatumLycoperdon giganteum

1970s Jet Spore Sampler (samples onto culture plates or plant leaves)2016 Jet Spore Sampler fan assembly2016 Jet Spore Sampler (samples into diagnostic tubes)New technology & opportunities Jet Spore SamplerJet Spore Sampler Modifications at Burkard Manufacturing Co (Stuart Wili) July 2016Prototype 2 Initial tests showed poor collection efficiency (