Service and Collaboration

Summary Section 7Introduction 68Exosome and Microvesicle Isolation 69EV protein quantifi cation, sceening and profi ling 69Nanoparticle Tracking Analysis (NTA) 70EV Nucleic Acid isolation 70Nucleic Acid profi ling and NGS 71Custom Products 72Additional services and project collaborations 72

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Service and Collaboration

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For information and customer support

contact us at:

info@hansabiomed.eu

• EV purifi cation and services

performed by experienced scientists

and qualifi ed personnel.

• Material is processed quickly.

• HansaBioMed Life Sciences has all

the necessary facilities for exosome

purifi cation, protein and nucleic acid

profi ling.

Being the oldest company entirely dedicated to exosome research, since 2007 HansaBioMed Life Sciences has developed unique competences and tools al-lowing us to help you with your research work. Our experienced scientists will help you accelerate your research and achieve your objectives quickly, providing consultancy and/or professional services using state of the art equipment. Our services include:

IntroductionCustomer support

Advantages

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HansaBioMed Life Sciences provides high expertise in Exosome and Microvesi-cle purifi cation from human or animal biofl uids and cell culture media (HBM-LS can provide both cell growth and EV isolation or EV isolation from supernatant collected by customer). Exosome isolation can be obtained following our stan-dard protocols which combine tangential fl ow fi ltration and SEC, or via other methodologies, such as immunoaffi nity (latex beads) and ultracentrifugation. Isolated EVs are subsequently quantifi ed for total protein contents and validated by nano tracking analysis (NTA) with the Zetaview equipment (Particle Metrix). Purifi ed EVs can be shipped to the customer lyophilized or frozen, as required.

Exosome and Microvesicle Isolation

Service Description

Cell culture growth and EV isolation

Cell lines can be shipped to HBM, who provides cell growth and EV isolation.

Isolation by Ultracentrifugation and Microfi ltration

Method for isolating EV from a large amount of sample. Service includes EV isolation, NTA and protein content quantifi cation.

Isolation by Size Exclusion Chromatografy

Isoaltion from small volume (100 μl - 2 ml). Service includes EV isolation, NTA and protein content quantifi cation.

Isolation by Immunoaffi nityIsoaltion from small volume (500 μl - 10 ml). Th e method allows to enrich EV subpopulations. Service includes EV isola-tion, NTA and protein content quantifi cation.

EV protein quantifi cation, screening and profi ling

Exosome and Microvesicle Isolation

Together with RNA, proteins are an important source of biomarkers and poten-tial indicators of disease states. HansaBioMed provides services for detection, quantifi cation and profi ling of exosome proteins, which can be analyzed via Ex-oTEST ELISA, Mass spectrometry , FACS analysis or Western Blott ing.

EV protein quantifi cation, screening and profi ling

Service Description

EV ELISA profi ling

ELISA assay is an effi cient method for isolating and profi ling protein markers. HBM-LS can provide:1- Multiple profi ling of exosome markers from a single sample.2- Exosome marker profi ling of a large number of samples.3- Possibility to customize the ELISA plate for EV subpopula-tion enrichment.

FACS analysis of EV markers FACS analysis on pre-isolated EVs and EV isolation and FACS analysis.

Mass Spectrometry analysis of EV markes

Mass Spectrometry profi ling on pre-isolated EVs.

Western Blott ing analysis Western blott ing analysis and profi ling of EV markers.

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Nanoparticle Tracking Analysis service

EV Nucleic Acid Isolation

Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. NTA allows the determination of a size distribution profi le of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension. NTA service is performed by the use of Zetaview (Particle Metrix), which allows EV characterization by size distribution, concentration, zeta potential and pheno-typing.

EVs shutt le functional RNAs (75% miRNA) and genomic DNA. HansaBioMed Life Sciences provides a wide portf olio of kits and services for EV-associated RNAs and DNA isolation from human/ animal biofl uids (plasma, serum, urine, etc.) and cell culture media. Nucleic Acids (NA) are isolated with the HBM-LS proprietary technology and quantifi ed by Nanodrop analysis. Delivery time approximately 1-2 weeks.

EV Nucleic Acid Isolation

Service Description

EV-RNAs Isolation

EV-RNA is purifi ed by HBM-LS RNA extraction kits, which uses immunobeads for capturing EVs. Customers have the advan-tage to customize the immunobeads for enrichment of specifi c EV subpopulations1-Total RNA from overall EV population2-Total RNA from specifi c EV subpopulations

EV-DNA Isolation

DNA isolation is performed by HBM-LS kit. Th e service al-lows:1- Isolation of cf-DNA and EV-DNA2- Isolation of only EV-DNA 3- Isolation of genomic DNA contained in EV subpopulations.

EV Nucleic Acid profi ling and Next Generation Sequencing

Hansabiomed Life Sciences scientists can provide an expert service for the iden-tifi cation of exosome-associated RNA/DNA biomarkers leveraging the through-put and scalability of Ion Torrent and SOLiD System (Th ermo Fisher Scientifi c) next-generation sequencing (NGS) platf orms. Th is service is an easy solution for researchers who are interested in identifying new exosome RNA biomarkers or in the profi ling expression of such biomarkers in the exosomes of their model cellular systems or patient biofl uids. You will receive your data back in approxi-mately 4-6 weeks.

Nucleic Acid profi ling and Next-Generation Sequencing

Service Description

Profi ling of EV associated NA by qPCR.

Profi ling of RNA/DNA exosome markers. Probes can be de-signed by the customer and sent to HBM-LS.

Next-Generation Sequencing (NGS)

Service is performed using Ion Torrent and SOLiD System (Th ermo Fisher Scientifi c) NGS platf orms.

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HansaBioMed Life Sciences can design and create a personalized kit by choosing from a wide variety of reagents and tools in our catalog.

Custom product

Product Description

Custom ELISA immunoplate

ELISA plates can be covalently coated with EV-binding anti-bodies.1- Trasparent, white and black plates are available.2- Coating antibody can be chosen by our antibody list (see section 6) or sent to HBM by customers.

Custom immunobeads

Latex immunobeads will be covalently coupled with the antibodies chosen from our antibody list, or sent to HBM-LS by customers.We provide the following product sizes:1- Immunobeads for 10 reactions2- Immunobeads for 20 reactions3- Immunobeads for 50 reactions

Custom ExoTESTCustomer can customize our ExoTEST platf orm chosing ELISA plate and detection antibody (see section 3, Quantifi cation kits)

For other Custom made solutions and for information contact us at info@hansabiomed.eu or visit our website

Custom Product

Service and project collaboration

HansaBioMed Life Sciences is open for collaboration with private or academic partners in research activites related to diff erent fi elds of exosome research and applications. Contact us for:

Service and project collaboration

Service Description

Biomolecule labeling and modifi cation

We can provide biomolecule modifi cations such as: protein labeling with fl uorescence dyes, conjuga-tion of biomolecules with affi nity ligands, PEGyation of biomolecules, immobilization of biomolecules on diff erent surfaces (hydrogels, molded polymers, synthetic membranes) etc.

Development of new assays for exosome marker identifi cation

We can develop personalized ELISA assays for a specifi c exosome protein marker quantifi cation or qPCR assays for identifi cation of miRNA and DNA markers.

Development of tools for capturing and enriching specifi c exosome subpopula-tions

We can develop tools for capturing and isolating specifi c exosome subpopulations to improve com-prehensive exosome profi ling in diseases.

Projects and collaborations and technical consulting

Contact us for: OEM product development, cell free regenerative medicine, collaboration in research projects (Horizon 2020, Eurostars, NIH grants, etc).

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