Series II 2100 bioanalyzer kits

Superior RNA 6000 assays and reagents

2

Improvements of the RNA assays

• Improved RNA ladder which is now included in both RNA kits

• Consistently higher sensitivity for the RNA Pico assay

• Increased assay stability for samples with a high salt content for both RNA assays

• New quantitation calibration for both RNA assays

• Larger marker volumes included

• Shelf life initially increased to at least 4 months. Extension is being tested

• Reagents are conveniently packaged according to storage temperature

3

New RNA ladder

RNA ladder now included in the kits

Ladder can also be ordered separately

Provides more intense and stable bands

More reliable integration and quantitation with the 2100 expert software

Series II RNA 6000 Nano assay

Old RNA 6000 Nano assay

Ladd

er

4

Increased sensitivity

50 pg/µL total RNA in water was analyzed using the Series II RNA 6000 Pico assay

Normal view Enhanced RNA view with the expert SW

Sensit

ivity

5

Salt tolerabilty Series II assay tolerates high amounts of salt

RNA 6000 Pico - Series II

water 50 mM Tris 10 mM MgCl2 50 mM NaCl

RNA 6000 Pico - old 1 ng/µL total RNA was run in different salt-containing media

water 50 mM Tris 10 mM MgCl2 50 mM NaCl

Salt to

leran

ce

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Salt tolerabilty Salt affect overall signal intensity

water 50 mM Tris 10 mM MgCl2 50 mM NaCl

RNA Pico - old - 1 ng total RNA was run in different salt-containing media

water 50 mM Tris 10 mM MgCl2 50 mM NaCl

Salt to

leran

ce

RNA 6000 Pico - Series II

7

Concentration accuracy

- Rat heart Total RNA (in water) was run in 5 different concentrations (2x each per chip) on 12 replicate chips (6 instruments x 2 different chip lots) – All samples (and Ladder) were pipetted individually as with standard protocol.

Average Quantification Rat heart Total RNA

0.0

100.0

200.0

300.0

400.0

500.0

600.0

700.0

400 310 230 170 75

UV Target [ng/µl]

Ave

rag

e Q

uan

tifi

cati

on

Original

Series II

UV Target

The quantitation calibration has been adjusted to better fit UV measures. Together with the more stable RNA ladder it provides for a more accurate and robust quantitation

Quant

itatio

n

8

RIN reproducibility

-Burkitt‘s Lymphoma Total RNA was run in 4 different conc. (1x each per chip) on 18 replicate chips (6 instruments x 3 different chip lots)

-Mouse Ovary Total RNA (partially degraded) in 5 different conc. (2x each per chip) on 12 replicate chips (6 instruments x 2 different chip lots)

290 ng/µl 140 ng/µl 70 ng/µl 35 ng/µlAverage RIN 9.9 10.0 10.0 10.0Stv. 0.1 0.0 0.1 0.1% Stdv. 0.7 0.4 0.5 1.1Average RIN. 9.8 10.0 9.9 9.9Stv. 0.1 0.1 0.2 0.1% Stdv. 0.6 0.7 1.9 0.9

RNA Concentration

Original

Series II

495 ng/µl 390 ng/µl 290 ng/µl 180 ng/µl 100 ng/µlAverage RIN 4.6 5.0 4.7 5.0 5.8Stv. 0.3 0.4 0.3 0.4 0.4% Stdv. 5.6 8.5 6.7 7.3 6.7Average RIN. 4.9 4.8 5.0 5.5 5.8Stv. 0.4 0.3 0.4 0.4 0.4% Stdv. 7.6 6.9 8.6 7.1 6.2

Original

Series II

RNA Concentration

Quant

itatio

n

RNA Integrity Number (RIN) analysis is unchanged from old to new kits. The variability is within the expected range across the conc. range.

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RNA 6000 Series II- SpecificationsSpecif

icatio

ns

RNA 6000 Nano RNA 6000 Nano Series II

RNA 6000 Pico RNA 6000 Pico Series II

Quantitative range

Total RNA:25–500 ng/µL mRNA:25-250 ng/µL

Total RNA:25–500 ng/µL mRNA:25-250 ng/µL

Qualitative range

Total RNA:5–500 ng/µL mRNA:25–250 ng/µL

Total RNA:5–500 ng/µL mRNA:25–250 ng/µL

Total RNA:200–5000 pg/µLmRNA:500-5000 pg/µL

Total RNA: 50–5000 pg/µl (water)mRNA:250–5000 pg/µl (water)

QuantitationReproducibility

10% CV 10% CV 20% CV

Quantitation accuracy

20% CV* 30% CV*

Buffer compatibility

TE 100 mM Tris or 125 mM NaCl or 15 mM MgCl2

TE 50 mM Tris or 50 mM NaCl

* ladder as sample

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Former Products Order Number Series II Products

Order Number US Ref. Price ($)

RNA 6000 Nano LabChip Kit

5065-4476 RNA 6000 Nano Kit

5067-1511 544*

RNA 6000 Nano Cooled Reagents

5065-4475 RNA 6000 Nano Reagents

5067-1512 296*

RNA 6000 Nano Ladder

5067-1529 150

RNA 6000 Pico LabChip Kit

5065-4473 RNA 6000 Pico Kit

5067-1513 587*

RNA 6000 Pico Cooled Reagents

5065-4472 RNA 6000 Pico Reagents

5067-1514 277*

RNA 6000 Pico Ladder

5067-1535 80

Ordering & pricing

Former kits will be obsolete June 30, 2006

*New price reflects inclusion of the RNA ladder

Order

ing

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Storage at 4C Storage at -20C

Packaging & storagePacka

ging

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SW update and assay download

Prerequisites to use the Series II RNA kits:

• 2100 expert software B.01.02 or B.02.02 must be installed

• New RNA assays and firmware needs to be installed, use Assay Update

• Assay Update patch can be downloaded free of charge or requested on CD (fax return form included in every kit)

Three options for software updates available:

• For biosizing users, free of charge update to the intro version of the 2100 bioanalyzer expert SW (Revision B.01.02)

• For B.02.01 users, the upgrade to B.02.02 is FREE of charge

• For all other users (i.e. beta versions), an update to the most current 2100 bioanalyzer expert SW (Revision B.02.02) for a minimal fee is recommended (order G2946CA)

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Summary

• Improved RNA ladder now included in both RNA kits

• Consistently higher sensitivity for the RNA Pico assay

• Increased assay stability for samples with a high salt content for both RNA assays

• New quantitation calibration for both RNA assays

• Larger marker volumes included

• Shelf life initially increased to at least 4 months

• Reagents conveniently packaged according to storage temperatures

• New Assay Update CD available free of charge

• Only compatible with 2100 expert software B.01.02 or B.02.02

• Offers for SW updates available

More detailed information at http://www.agilent.com/chem/2100-new-assays!