seminar ppt
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Identification of Genes required for
Cytoplasmic Localizationin
Early C. elegans Embryos
Background
Development of C.elegans
Development• After fertilization, the two pronucleis join together.
http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt
Development• Then the mitotic spindle forms, and it migrates posteriorly.
http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt
Development• Owing to the migration, the two daughter blastomeres are produced in diff
erent sizes after the first cleavage.
http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt
Development• At the second cleavage, AB divides transversely and P1 divides
longitudinally. AB always divides before P1.
http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/movies.cfm/kjk1_wt
Principles of Development (Lewis Wholper) Chapter 5
• After fertilization, the two pronucleis join together.
• Mitotic spindle forms, and it migrates posteriorly. • Daughter blastomeres are produced in different
sizes after the first cleavage.• AB> P1• P granules are localized to P1• At the second cleavage, AB divides transversely
and P1 divides longitudinally. • AB always divides before P1• P granules are localized to P2
• After fertilization, the two pronucleis join together.
• Mitotic spindle forms, and it migrates posteriorly. • Daughter blastomeres are produced in different
sizes after the first cleavage.• AB> P1• P granules are localized to P1• At the second cleavage, AB divides transversely
and P1 divides longitudinally. • AB always divides before P1• P granules are localized to P2
The Result
Genes
Defects
• Equal First Cleavage
• Altered Orientation of Second Cleavages
• Synchronous Cleavages
• Abnormal Localization of P Granule
Aim
To identify the genes required for cytoplasmic localization
in early C. elegans embryos
Sub-Questions
http://www.mbg.cornell.edu/cals/mbg/research/kemphues-lab/research-details.cfm
Identify The Genes
Screening There are four categories of mutation, but three of them
are hard to observe.
So they chose the forth.
Linkage Mapping Linkage Analysis
Meiotic Recombination
Complementation
Identify The Defects
• Equal First Cleavage
• Altered Orientation of Second Cleavages
• Synchronous Cleavages
• Abnormal Localization of P Granule
Equal First Cleavage
Equal First Cleavage
• Blastomere Size Measurement
• Spindle Movement Measurement
Equal First Cleavage
Equal First Cleavage
Altered Second Cleavages
Altered Second Cleavages
Altered Second Cleavages
Synchronous Cleavages
Synchronous Cleavages
• par-1 and par-4 cleave synchronously up until fourth or fifth cleavage
• par-2 and par-3 are rather earlier
Abnormal Localization of P Granule
Abnormal Localization of P Granule
Abnormal Localization of P Granule
Identify The Phenotype
• Differentiated Cell Types
• Grandchildless
The Result
Summary