semester two examinations, 2018 institute of … · page 1 of 13 arholiadau examinations semester...

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ARHOLIADAU EXAMINATIONS

SEMESTER TWO EXAMINATIONS, 2018 INSTITUTE OF BIOLOGICAL, ENVIRONMENTAL AND RURAL SCIENCES BR13720 MOLECULAR BIOCHEMISTRY MAE MODD I FYFYRWYR GYFLWYNO ATEBION I’R PAPUR HWN NAILL AI YN Y GYMRAEG NEU'R SAESNEG STUDENTS MAY SUBMIT ANSWERS TO THIS PAPER IN EITHER WELSH OR ENGLISH Time Allowed: 2 hours ANSWER ALL QUESTIONS

CALCULATORS ARE REQUIRED

Rhif Cyfeirio’r Myfyriwr/

Student Reference Number:

Dyddiad/Date

Ystafell Arholiad/Examination Room

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1. Rhowch y wybodaeth angenrheidiol yn y blychau uchod a rhowch eich enw yn y man gwag ar dop y tudalen ar y dde. Plygwch a seliwch y cornel ochr dde ar dop y tudalen (gan ddefnyddio’r slip gludiog a ddarperir) er mwyn cuddio eich enw.

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5. Os ymadewch â’r ystafell arholi yn gynnar, gwnewch hynny’n dawel os gwelwch yn dda rhag aflonyddu ar ymgeiswyr eraill.

1. Please give the information requested above and enter your name in the space provided at the top right of this page. Fold over and seal down the top right-hand corner (using the gummed slip provided) in order to conceal your name.

2. The answers must be legibly written. The Examiners can either ignore illegible script or have it typed and the expense of typing charged to the candidate.

3. Write your answers in ink.

4. This paper must be handed in complete.

5. If you leave the examination early, please do so quietly so as not to disturb other candidates.

Fold over and stick down/plygwch y gornel drosodd

Cyfenw/Surname ............................................

Enwau Cyntaf/

Forenames ......................................................

.......................................................................

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1. The following is a structure of Penicillin G. Circle and name four functional groups in

the molecule. (4 marks)

2. Draw and name the following compounds. (6 marks)

a. CH3CH2CH2CH2COCH3

b. CH3CH2CH2CH2CH2CH2CHO

c. CH3CH2CH2CONH2

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3. What is the pH of each of the following solutions? (7 marks)

a. 3.2x10-3 M HBr

b. 3.70x10-4 M NaOH

c. A 0.15 mol L-1 solution of ammonia if the Kb for ammonia = 1.8 x 10-5?

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4. Give the hybridisation and bond angles for each carbon atoms in the following

compound?

(6 marks)

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5. Draw the equilibria (including conjugate acids and conjugate bases) for the ionisation

of these acids and explain the difference between the pKa values. (6 marks)

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6. Assign each of the following compounds as either E or Z isomers. (4 marks)

a.

b.

c.

d.

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7. Assign each of the following compounds as R or S configuration. (6 marks)

a.

b.

c.

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8. High resolution mass spectrometry showed an unknown metabolite ion with an

accurate mass of m/z 269.0881 containing elements from the following list:

1H : 1.0078 12C: 12.0000

14N : 14.0031 16O: 15.9949

The instrument has 3 ppm accuracy. Calculate the accurate mass (m/z) of the ions and

circle the one of the two following metabolites which has been analysed.

C9H17O9 mannosylglycerate [M+H]+

C10H13N4O5 Inosine [M+H]+ (3 marks)

9. Give oxidation numbers for each of the atoms in the following molecule: (4 marks)

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10. The following is a form of the Nernst equation:

a. Define the terms Eh and Eo. (2 marks)

b. The value of Eo for the ferricyanide/ferrocyanide couple at 25oC is 0.43 V. Calculate

the value of Eh of a solution containing 0.1 M potassium ferricyanide and 0.01M

potassium ferrocyanide (R = 8.314 J mol-1K-1, F = 96485 coulombs mol-1). (5 marks)

Eh

= Eo + –––– 2.303 log ––––– [Aox] [Ared]

RT

nF

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11. Provide descriptions of the two graphs (in top rectangles) pertaining to analyses of

enzyme kinetics and label the axes. (6 marks)

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12. The following is a 1H NMR spectrum of a compound with a molecular formula

C5H11Br. Identify the peaks in the spectrum and the compound. (5 marks)

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13. Different types of carbonyl groups have different electrophilic reactivity. Give

explanations (using diagrams where appropriate) for the positions of esters, acid

chlorides, amides in this rank order relative to ketones. (6 marks)

END OF PAPER

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