self-organizing bio- structures nb2-2008 l. duroux
TRANSCRIPT
![Page 1: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/1.jpg)
Self-Organizing Self-Organizing Bio-structuresBio-structures
NB2-2008NB2-2008
L. DurouxL. Duroux
![Page 2: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/2.jpg)
Lecture 7Lecture 7
Protein-based nanomaterialsProtein-based nanomaterials
![Page 3: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/3.jpg)
1. Peptide-based 1. Peptide-based nanostructuresnanostructures
![Page 4: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/4.jpg)
A first insight into SA A first insight into SA peptidespeptides
Concept of peptide SA introduced by Concept of peptide SA introduced by Ghadiri et al. (1993)Ghadiri et al. (1993)
Synthetic cyclic polypeptides (alternate Synthetic cyclic polypeptides (alternate L- & D-) self-assemble into Ø8-9nm L- & D-) self-assemble into Ø8-9nm nanotubesnanotubes
Function as novel antimicrobial agents, Function as novel antimicrobial agents, drug delivery systems & nanomaterialsdrug delivery systems & nanomaterials
![Page 5: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/5.jpg)
Ghadiri’s cyclic Ghadiri’s cyclic polypeptides (CPP)polypeptides (CPP)
![Page 6: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/6.jpg)
Electronic microscopyElectronic microscopy
![Page 7: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/7.jpg)
pH-dependance of CP SApH-dependance of CP SA
![Page 8: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/8.jpg)
CPP forming pores in CPP forming pores in membranesmembranes
![Page 9: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/9.jpg)
cyclic-peptides self-assembled into open tubes
consist of an even number of alternated D / L amino acids
formation of anti-parallel hydrogen bonded network
assembly could be controlled by electrostatic interactions
assembly could be directed toward particular environments (hydrophobic) by selection of amino acids
are functional material (ion channel & antibiotic)
Self-Assembling Peptide Nanotubes
![Page 10: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/10.jpg)
SA based on SA based on native 2native 2ndaryndary
structural motifsstructural motifs
![Page 11: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/11.jpg)
Protein structural motifs & SA Protein structural motifs & SA designs designs
Type II polyPro helixAmyloid fibrils
![Page 12: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/12.jpg)
SA Fibers engineering based SA Fibers engineering based on coiled-coilson coiled-coils
Woolfson & Ryadnov, 2006
![Page 13: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/13.jpg)
Amyloid peptidesAmyloid peptides
A generic, universal form of A generic, universal form of protein/peptide aggregationprotein/peptide aggregation
Cause of many diseases: Cause of many diseases: Altzheimer’s, Type II diabetes, Altzheimer’s, Type II diabetes, Prions...Prions...
Extended Extended -sheet SA forming fibrils-sheet SA forming fibrils
![Page 14: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/14.jpg)
Nano-object formed by Nano-object formed by amyloid peptidesamyloid peptides
Object formed
Amyloid fibrils (pancreas type II diabetes)
Amyloid fibrils
Nanotubes
Nanospheres
![Page 15: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/15.jpg)
The role of aromatics in The role of aromatics in amyloid fibrils formationamyloid fibrils formation
Phe dipeptide: the recognition core of Altzheimer’s amyloid fibril
Forms nanotubes
Applications in nano-electronics
![Page 16: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/16.jpg)
SA based on SA based on amphiphilicityamphiphilicity
![Page 17: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/17.jpg)
Structures of peptides used Structures of peptides used in SAin SA
Reches and Gazit, 2006
Boloamphiphile
Amphiphile
Surfactant-like
Phenylalaninedipeptide
![Page 18: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/18.jpg)
Peptide nanotubes Peptide nanotubes
Applications
Nanotubes with Ca-binding and cell-adhesion bone-like material
Idem, non-conjugated
Nanofibers forming hydrogel matrix for tissue regeneration & engineering
![Page 19: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/19.jpg)
Peptide-Amphiphile and Peptide-Amphiphile and Tissue EngineeringTissue Engineering
SA fibers with CCCCGGGS(PO4)PGD: without Ca2+ (a) and Ca2+ (b)
![Page 20: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/20.jpg)
Aromatic Aromatic dipeptidesdipeptides
![Page 21: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/21.jpg)
Hydrophobic layers made with Hydrophobic layers made with dipeptidesdipeptides
Görbitz, 2006
![Page 22: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/22.jpg)
Types of nanostructures Types of nanostructures from various dipeptidesfrom various dipeptides
![Page 23: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/23.jpg)
SA of Val-Ala classSA of Val-Ala class
![Page 24: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/24.jpg)
SA patterns of the Phe-SA patterns of the Phe-Phe classPhe class
Phe-Trp Phe-Gly
Phe-Leu Phe-Phe
![Page 25: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/25.jpg)
Formation of nanotubes Formation of nanotubes with Phe-Phe dipeptideswith Phe-Phe dipeptides
![Page 26: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/26.jpg)
2. Protein-based 2. Protein-based SA nanotoolsSA nanotools
![Page 27: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/27.jpg)
S-layer proteinsS-layer proteins
![Page 28: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/28.jpg)
What are S-Layer What are S-Layer proteins?proteins?
S stands for surface: glycoprotein subunits S stands for surface: glycoprotein subunits forming outer envelope of Bacteria and Archeaforming outer envelope of Bacteria and Archea
Periodic structures with defined physico-Periodic structures with defined physico-chemical properties (pore size)chemical properties (pore size)
Self_assemble into 2D layers to form Self_assemble into 2D layers to form monomolecular lattices: potential in monomolecular lattices: potential in nanobiotechnologies (scaffolds, patterning nanobiotechnologies (scaffolds, patterning matrices)matrices)
![Page 29: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/29.jpg)
Applications of S-layersApplications of S-layers1. production of isoporous ultrafiltration membranes2. supporting structures for defined immobilization or
incorporation of functional molecules (e.g. antigens, antibodies, ligands, enzymes)
3. matrix for the development of biosensors including solid-phase immunoassays and label-free detection systems
4. Support and stabilizing matrices for functional lipid membranes, liposomes, and emulsomes
5. adjuvants for weakly immunogenic antigens and haptens
6. Matrix for controlled biomineralization and structure for formation of ordered arrays of metal clusters or nanoparticles (molecular electronics and nonlinear optics or catalysts)
![Page 30: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/30.jpg)
S-Layer latticesS-Layer lattices
Gram+ bacterium
100nm
![Page 31: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/31.jpg)
Self-Assembled Self-Assembled monomolecular layersmonomolecular layers
![Page 32: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/32.jpg)
S-layer as template for PSA S-layer as template for PSA detectiondetection
![Page 33: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/33.jpg)
Assembly of lipids on S-Assembly of lipids on S-layerslayers
Non-covalent bondingNon-covalent bonding
Electrostatic interactions between Electrostatic interactions between corrugated (inner) side of S-layer corrugated (inner) side of S-layer (carboxy groups) and charges on lipid (carboxy groups) and charges on lipid head groups (zwitterions)head groups (zwitterions)
2-3 contact points between protein 2-3 contact points between protein and lipid: most lipids free to diffuse and lipid: most lipids free to diffuse laterally: semi-rigid membranelaterally: semi-rigid membrane
![Page 34: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/34.jpg)
S-layers as support for lipid S-layers as support for lipid membranesmembranes
![Page 35: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/35.jpg)
Self-Assembly of a ion-Self-Assembly of a ion-channel in channel in
S-layersS-layers
![Page 36: Self-Organizing Bio- structures NB2-2008 L. Duroux](https://reader036.vdocuments.mx/reader036/viewer/2022062313/56649cb75503460f9497d2d0/html5/thumbnails/36.jpg)
Expected applications of S-Expected applications of S-layer-driven SA of lipid layer-driven SA of lipid
membranesmembranes Life Sciences:Life Sciences:
Drug deliveryDrug delivery DiagnosticsDiagnostics BiosensorsBiosensors
Chemistry and material sciencesChemistry and material sciences Bio-mineralizationBio-mineralization Non-linear opticsNon-linear optics Molecular electronicsMolecular electronics CatalysisCatalysis