segmentation results

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7/23/2019 Segmentation Results http://slidepdf.com/reader/full/segmentation-results 1/37 Segmentation of Cysts from OCT images Dataset: OCT image each of 49 Frames of OPTIMA CYST CHALLENGE. Vendor: Spectrail Heidelberg Engineering Slides 3-4 are frame number 1 of bscan1 without Cyst. Slides 5-6 are frame number 19 of bscan1 with Cyst. Slides 7-16 are Cyst segmentation results from Bscan_1 of a Spectrail_1 using Infinity Reconstruction of log+gray weight difference. Slides 17-22 are Cyst segmentation results from Bscan_1 of a Spectrail_2 using Infinity Reconstruction of log+gray weight difference. Slides 26-35 are Cyst segmentation results from Bscan_1 of a Spectrail_2 using Fuzzy C means Clustering.

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Page 1: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 1/37

Segmentation of Cysts from OCT images

Dataset: OCT image each of 49 Frames of OPTIMA CYST CHALLENGE.

Vendor: Spectrail Heidelberg Engineering

Slides 3-4 are frame number 1 of bscan1 without Cyst.

Slides 5-6 are frame number 19 of bscan1 with Cyst.

Slides 7-16 are Cyst segmentation results from Bscan_1 of a Spectrail_1

using Infinity Reconstruction of log+gray weight difference.

Slides 17-22 are Cyst segmentation results from Bscan_1 of a Spectrail_2

using Infinity Reconstruction of log+gray weight difference.

Slides 26-35 are Cyst segmentation results from Bscan_1 of a Spectrail_2

using Fuzzy C means Clustering.

Page 2: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 2/37

Results

De-noised OCT Image: Method OBNLM

Cyst

Page 3: Segmentation Results

7/23/2019 Segmentation Results

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Nerve Fiber Layer(NFL)

Retinal Pigment Epithelium (RPE)

ROI Drawn of Nerve Fiber Layer and Retinal

Pigment Epithelium layers Using Gradient

Based Spectra Peaks Detection algorithm

Page 4: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 4/37

Nerve Fiber Layer(NFL)

Retinal Pigment Epithelium (RPE)

Segmented Nerve Fiber Layer and Retinal

Pigment Epithelium layers Using Gradient

Based Spectra Peaks Detection based method

Page 5: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 5/37

Nerve Fiber Layer(NFL)

Retinal Pigment Epithelium (RPE)

ROI Drawn of Nerve Fiber Layer and Retinal

Pigment Epithelium layers Using Gradient

Based Spectra Peaks Detection algorithm

Page 6: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 6/37

Nerve Fiber Layer(NFL)

Retinal Pigment Epithelium (RPE)

Segmented Nerve Fiber Layer and Retinal

Pigment Epithelium layers Using Gradient

Based Spectra Peaks Detection based method

Page 7: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 7/37

Semi-Automatic Segmentation of Cyst

Page 8: Segmentation Results

7/23/2019 Segmentation Results

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RED Marks are Original Ground truth by Ophthalmologist

Semi-Automated method employed one pixel selected from each cyst to segment

Image: Bscan_1 of Spectrails 1; Frame No: 14

Page 9: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 9/37

Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Image: Bscan_1 of Spectrails 1; Frame No: 14

Red marked regions are Segmented result and white regions is Ground Truth

Page 10: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 10/37

RED Marks are Original Ground truth by Ophthalmologist

Semi-Automated method employed one pixel selected from each cyst to segment

Image: Bscan_1 of Spectrails 1; Frame No: 13

Page 11: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 11/37

Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Image: Bscan_1 of Spectrails 1; Frame No: 13

Red marked regions are Segmented result and white regions is Ground Truth

Page 12: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 12/37

RED Marks are Original Ground truth by Ophthalmologist

Semi-Automated method employed one pixel selected from each cyst to segment

Image: Bscan_1 of Spectrails 1; Frame No: 24

i l f f k i i fi i i h d

Page 13: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 13/37

Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Image: Bscan_1 of Spectrails 1; Frame No: 24

Red marked regions are Segmented result and white regions is Ground Truth

RED M k O i i l G d h b O h h l l i

Page 14: Segmentation Results

7/23/2019 Segmentation Results

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RED Marks are Original Ground truth by Ophthalmologist

Semi-Automated method employed one pixel selected from each cyst to segment

Image: Bscan_1 of Spectrails 1; Frame No: 25

S t ti lt f t f M k i t i I fi it R t ti M th d

Page 15: Segmentation Results

7/23/2019 Segmentation Results

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Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Image: Bscan_1 of Spectrails 1; Frame No: 25

Red marked regions are Segmented result and white regions is Ground Truth

RED M k O i i l G d t th b O hth l l i t

Page 16: Segmentation Results

7/23/2019 Segmentation Results

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RED Marks are Original Ground truth by Ophthalmologist

Semi-Automated method employed one pixel selected from each cyst to segment

Image: Bscan_1 of Spectrails 1; Frame No: 19

S t ti lt f t f M k i t i I fi it R t ti M th d

Page 17: Segmentation Results

7/23/2019 Segmentation Results

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Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Image: Bscan_1 of Spectrails 1; Frame No: 19

Red marked regions are Segmented result and white regions is Ground Truth

RED Marks are Original Ground truth by Ophthalmologist

Page 18: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 18/37

RED Marks are Original Ground truth by Ophthalmologist

Semi-Automated method employed one pixel selected from each cyst to segment

Image: Bscan_1 of Spectrails 2; Frame No: 21

Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Page 19: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 19/37

Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Image: Bscan_1 of Spectrails 2; Frame No: 21

Red marked regions are Segmented result and white regions is Ground Truth

RED Marks are Original Ground truth by Ophthalmologist

Page 20: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 20/37

RED Marks are Original Ground truth by Ophthalmologist

Semi-Automated method employed one pixel selected from each cyst to segment

Image: Bscan_1 of Spectrails 2; Frame No: 25

Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Page 21: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 21/37

Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Image: Bscan_1 of Spectrails 2; Frame No: 25

Red marked regions are Segmented result and white regions is Ground Truth

RED Marks are Original Ground truth by Ophthalmologist

Page 22: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 22/37

RED Marks are Original Ground truth by Ophthalmologist

Semi-Automated method employed one pixel selected from each cyst to segment

Image: Bscan_1 of Spectrails 2; Frame No: 26

Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Page 23: Segmentation Results

7/23/2019 Segmentation Results

http://slidepdf.com/reader/full/segmentation-results 23/37

Segmentation result of cysts from Marker points using Infinity Reconstruction Method

Image: Bscan_1 of Spectrails 2; Frame No: 26

Red marked regions are Segmented result and white regions is Ground Truth

Page 24: Segmentation Results

7/23/2019 Segmentation Results

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Automatic Segmentation of Cyst

Automatic Segmentation of cysts using FCM Clustering Method

Page 25: Segmentation Results

7/23/2019 Segmentation Results

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Automatic Segmentation of cysts using FCM Clustering Method

Image: Bscan_1 of Spectrails 1; Frame No: 21

Red marked regions are Segmented result and white regions is Ground Truth

Automatic Segmentation of cysts using FCM Clustering Method

Page 26: Segmentation Results

7/23/2019 Segmentation Results

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Automatic Segmentation of cysts using FCM Clustering Method

Image: Bscan_1 of Spectrails 1; Frame No: 22

Red marked regions are Segmented result and white regions is Ground Truth

Automatic Segmentation of cysts using FCM Clustering Method

Page 27: Segmentation Results

7/23/2019 Segmentation Results

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Automatic Segmentation of cysts using FCM Clustering Method

Image: Bscan_1 of Spectrails 1; Frame No: 24

Red marked regions are Segmented result and white regions is Ground Truth

Automatic Segmentation of cysts using FCM Clustering Method

Page 28: Segmentation Results

7/23/2019 Segmentation Results

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Automatic Segmentation of cysts using FCM Clustering Method

Image: Bscan_1 of Spectrails 1; Frame No: 25

Red marked regions are Segmented result and white regions is Ground Truth

Automatic Segmentation of cysts using FCM Clustering Method

Page 29: Segmentation Results

7/23/2019 Segmentation Results

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Automatic Segmentation of cysts using FCM Clustering Method

Image: Bscan_1 of Spectrails 2; Frame No: 22

Red marked regions are Segmented result and white regions is Ground Truth

Automatic Segmentation of cysts using FCM Clustering Method

Page 30: Segmentation Results

7/23/2019 Segmentation Results

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Automatic Segmentation of cysts using FCM Clustering Method

Image: Bscan_1 of Spectrails 2; Frame No: 25

Red marked regions are Segmented result and white regions is Ground Truth

Automatic Segmentation of cysts using FCM Clustering Method

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7/23/2019 Segmentation Results

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g y g g

Image: Bscan_1 of Spectrails 3; Frame No: 21

Red marked regions are Segmented result and white regions is Ground Truth

Automatic Segmentation of cysts using FCM Clustering Method

Page 32: Segmentation Results

7/23/2019 Segmentation Results

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g y g g

Image: Bscan_1 of Spectrails 3; Frame No: 25

Red marked regions are Segmented result and white regions is Ground Truth

Automatic Segmentation of cysts using FCM Clustering Method

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g y g g

Image: Bscan_1 of Spectrails 4; Frame No: 24

Red marked regions are Segmented result and white regions is Ground Truth

Automatic Segmentation of cysts using FCM Clustering Method

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g y g g

Image: Bscan_1 of Spectrails 4; Frame No: 35

Red marked regions are Segmented result and white regions is Ground Truth

Automatic Segmentation of cysts using FCM Clustering Method

Page 35: Segmentation Results

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Image: Bscan_1 of Spectrails 4; Frame No: 33

Red marked regions are Segmented result and white regions is Ground Truth

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Results and Discussion

• Results: – Results obtained so far using   “Mathematical   Morphological Infinity 

Reconstruction” method on log of gray weight difference matrix showsthat method is robust to differentiate the gradient differencesbetween cyst and retinal Layers.

 – Above method fails to achieve accurate segmentation due to smallgradient differences.

 – The contour of cyst detection is challenge due to artifacts such asNoise and gray level similarities with Retinal Layers.

 –   “Fuzzy   C Means   Clustering”  gives better results than log-gray weightdifference matrix method.

Page 37: Segmentation Results

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Future Work

• Future work to be done: – Improving the  “Mathematical   Morphological Infinity   Reconstruction” 

based segmentation either by Mathematical Morphological operations

or Fuzzy C Means clustering.

 – Employing Level Set for semi-automatic segmentation of cysts.

 – Developing an Automated method using level set contour detection

and inference mechanism.