seeking business development and sales role opportunities
TRANSCRIPT
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Currently Seeking Employment
Opportunities in business development
and sales
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Contact Rafael Casiano at 813-330-
7555rafaelcasiano2@
gmail.com
Top Commercial performer in 2016Built a very strong funnel and generated $8M
in contracts in Year 2018 Increased the # of customers from 15 in 2015
to 40 in 2016Exceeded 2016 plan by $3MFinished 2016, 3x higher revenue than 2015 Increased the # of customers from 3 in 2014 to
15 in 2015Double sales in 2015 vs. 2014
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Accelerate Discovery and Development from a Single Slide
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Challenges in Diagnostics Discovery and Development
Limited patient tissue
for comprehensiv
e analysis
Implications• Rapid sample depletion
• Insufficient data for stratification research
• Therapeutic options limited for patients
Protein Biomarkers• Protein expression• Phosphorylation • Ligands / Growth factors• Cell types
Genomic Biomarkers• DNA• RNA• miRNA• Sequencing
Complex Disease Biology• Interactions of multiple
pathways• Tumor heterogeneity• Adaptive resistance
MultiOmyx enables comprehensive profiling, improved diagnosis, and therapeutic options.
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Immunoprofiling-Hot Application for MultiOmyx
Immune classification color blends
CD4 CD8 CD68 PD-L1 pan-CK CD68|PD-L1
CD4 CD8 CD68 PD-L1 pan-CK
Immunofluorescence color blends
MultiOmyx enables profiling the types of immune cells present in the tumor
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Serial Sections (5µm)
FFPE Block
(10-30 µm)
Single Ab staining
CD4CD8CD19CD45ROKi67
Different sections of a tissue being analyzed for different biomarkers may lead to different results!
Challenges with assessing immune markers co-expression in singleplexed assay
Co-expression Analysis
MO Multiplexed assay enables analysis of multiple immune markers co-expression from a single slide
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Hyperplexed: stain, image, erase and repeat
Dye Inactivation>60
Proteins
PD-L1 PanCK
PD-L1 PanCK
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CD8+ Ki67+
CD4+ CD45RO+ CD8+ CD45RO+
CD8+ Granzyme B+CD8+ PD1+
“Memory” T Helper “Memory” TC
“effector” TC“Proliferating” TC “Anergic” TC
Immune cell co-expressionCD4+FoxP3+ “Regulatory” T cell
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Immune Cell Co-localization
PanCK CD3 CD4 CD8 FoxP3 PD-L1 CD68
Tumor/PanCK+
CD3+CD4+
T helper
CD3+CD4+FoxP3+
T regulatory
PD-L1+CD68+
CD3+CD8+
T cytotoxic
Tumor Margin
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Innate (tumor cell intrinsic) resistance Adaptive resistance
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VisualizationBiomarker overlays
Biomarker co-expression
• Visualize co-localization of markers
• Generate .png images for publications and presentations
• Evaluate biomarker co-expression
Biomarker heat maps
• Visualize heat maps of multiple biomarkers for assessment of expression and co-localization
Biomarker clusters
• Visualize results of cluster analysis and explore the biosignatures
Molecular H&E Molecular DAB
• Conversion of fluorescent image to a H&E appearance. Allows interpretation in context of tumor
• Conversion of fluorescent image to DAB appearance. Familiar format for pathologist
Cluster ID
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Low Immune
Biomarker
Cell Count
s
Density in
Tumor (#/mm
2)
Density in
Stroma (#/mm
2)Tumor Cells 1386 NA NA
CD4+ 64 23.3 177.4CD8+ 47 11.7 134.6
CD20+ 13 7.8 33.6FOXP3+ 53 7.8 156.0PDL1+ 0 0 0
Biomarker
High Immune
Cell Count
s
Density in
Tumor (#/mm2
)
Density in
Stroma (#/mm2)
Tumor Cells 1692 NA NACD4+ 231 238.1 675.4CD8+ 299 492.3 547.0
CD20+ 14 2.7 61.8FOXP3+ 177 160.5 556.5PDL1+ 234 246.1 675.4
Hig
h Im
mun
eLo
w Im
mun
e
Multiplexed IF Analytics Output
CK CD4 CD8 CD20 FOXP3 PD-L1
CK CD4 CD8 CD20 FOXP3 PD-L1
CK CD4 CD8 CD20 FOXP3 PD-L1
CK CD4 CD8 CD20 FOXP3 PD-L1
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Immune Infiltration
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MultiOmyx Tumor Infiltrating Lymphocyte Panel
O Provides quantitative analytical results and spatial information of 12 cancer immune markers from every cell on a single FFPE
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Dyes are cycled by a proprietary chemical inactivation methodGerdes et al (2013) PNAS
Multiplexed ProteinsProtein + DNA FISH
CD4 CD8 CD68 PD-L1 pan-CK
DNA Sequencing (ROI)Immune Phenotyping
- Up to 60 protein biomarkers can be fluorescently imaged in a single FFPE tissue section
- Additional “omics” such as RNAScope, DNA FISH, and NGS can be performed from the same IF processed slide and analyzed with protein data
- Advanced visualization of immune biomarker co-expression and co-localization
- Sophisticated algorithms identify individual cells and measure quantitative per-cell biomarker and morphology profiles
MultiOmyx Technology Summary…
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