SAFETY MEASURES WHILE WORKING WITH ESCHERICHIA COLI

PRESENTED BY:

WAJEEHA ABRAR R.NO-18M

ADEENA ASIFR.NO- 70R

They are bacteria found in environment, faeces, foods and intestines of people and animals.

E. coli can cause :• Diarrhea• Urinary tract infections• Respiratory illness• Bloodstream infections and other illnesses.

WHAT IS ESCHERICHIA COLI ?

• The best way to prevent spreading of E. coli to other people is to maintain proper hygiene.

• Clear your work area• Wipe off your lab table with

disinfectant solution.• Do not incubate plates for

more than the given time.

SAFETY MEASURES

• Do not touch your face when handling plates or tubes with E. coli .

• Wear gloves and lab coats.• Immerse instruments in

bleach solution to kill bacteria when working with bacterial cultures and dispose them in a BIOHAZARD bag.

• Wash hands regularly• Sterilize all instruments, solutions,

and media prior to using them for plating procedures.

• Clear away all materials cluttering your work area on the laboratory bench.

• Do not store food in lab.• Do not eat, drink, smoke etc• Do not mouth pipette.• Laboratory personnel must

be appropriately trained.• Wear safety glasses

Slide Title• Wash fruits and

vegetables thoroughly• Avoid cross-contamination

by using clean utensils, pans, and serving platters

• Defrosting meat in the refrigerator or microwave

• Use pasteurized milk (avoid raw milk)

• Avoid undercooked meat• Wash your hands after

contact with animals.

HOW TO AVOID INTESTINAL INFECTION DUE TO E.coli ?

http://2014.igem.org/Team:Bordeaux/Safety

http://www.cdc.gov/ecoli/index.html

http://www.slideshare.net/nomank992/e-coli-48345548

http://www.foodsafetynews.com/2014/10/cdc

REFERENCES