Updates to SABRN SC 2010

R. M. Chirwa

SABRN SC & Gender Workshop, held in Maputo, Mozambique – 3-8 October 2010

Network Breeding

Germplasm Development and ExchangeGenerating segregating populations: were advanced atGenerating segregating populations: were advanced at both sites Chitedze and BembekeNurseries : Chitedze 47 and Bembeke 36 nurseriesTrials: Yield trials were conducted for different objectives, and regional trials were distributed across countries. gBreeder Seed Production: Chitedze increased seed for all the ten released varietiesWinter Seed Multiplication-Liwonde: Seed increase for nurseries and segregating breeding lines – for distribution in 2010-11

Network Breeding

Single plant selections made during 2009-2010

Generation Important Traits Next Generation

# of plants selected.

F4 ALS, Zn& Fe F5 36F4 CBB, Zn & Fe F5 42F6 ALS F7 50F6 ALS F7 50F6 ALS F7 27F6 Drought resistance F7 19F6 CBB F7 5

SARBYT Genotype Rust Yield-kg ha-1 Rank colour size

VTTT 923/10 3 1 3310 1 S LVTTT 923/10-3 1 3310 1 Sugar LargeCIM-KHAK02-24-1 1 2341 2 Khaki SmallCIM-KHAK02-22-1-2 1 2254 3 Khaki SmallMC 12832-129-1 1 2196 4 Caroca SmallMC 12832-129-3 1 2095 5 Carioca SmallCIM-NAV02-11-1 2 2028 6 Navy SmallCIM-D-C01-30-1 1 2020 7 Cream LargeSER 45 1 1934 8 Red SmallCIM-D-C01-56-10 1 1916 9 Mwezimoja LargeCIM-D-C01-119-1 1 1895 10 Cream LargeRCB 237 1 1846 11 Red SmallCIM-KHAK02-30-1 4 1700 12 Khaki SmallCIM-KHAK02-30-1 4 1700 12 Khaki SmallCIM-D-C01-03-1 1 1686 13 Cream LargeGCI-CAL-172-AR 1 1677 14 Calima LargeSER 86 1 1519 15 Red SmallCIM D C01 59 1 1 1511 16 Cream LargeCIM-D-C01-59-1 1 1511 16 Cream LargeCIM-RM02-67-2 1 1458 17 Calima LargeCAL143 1 1040 18 Calima LargeLOCAL[SUAGAR 131] 1 960 19 Sugar LargeCANELLIN () 6 643 20 white LargeMean 2 1801CV% 43.6 55SE:+/_ 0.327 495.6

Small Seeded Drought Lines Genotype Yield-kg ha-1 Rust colour SizeGenotype Yield kg ha Rust colour SizeSER 117 662 1 Red SmallSER 121 689 2 Red SmallNCB 281 711 8 Red SmallSER 93 771 1 R d S llSER 93 771 1 Red SmallSER 124 781 1 Red SmallSER 51 791 1 Red SmallSER 79 806 1 Red SmallSER 52 850 1 Red SmallNCB 280 877 6 Red SmallSER 49 909 1 Red SmallSER 72 921 5 Red SmallSER 72 921 5 Red SmallSER 103 1309 1 Black SmallSER 116 1315 1 Red SmallSER 102 1400 1 Red SmallNCB 275 1443 2 Black SmallRCB 272 1477 1 Black SmallRCB 273 1493 1 Black SmallNCB 227 1748 1 Black SmallNCB 227 1748 1 Black SmallMean 1019 2CV% 22.3 39.7SE:+/_ 148 0.37

Calima Lines Genotype Yield-kg ha-1* Rust CBB colour SizeAND 1062 246 1 2 calima MediumCIM-RM00-10-3 911 1 1 calima LargeCIM RM00 211 4 927 1 1 calima LargeCIM-RM00-211-4 927 1 1 calima LargeCIM-RM02-136-1 933 1 1 calima MediumCIM-RM02-5-1 954 1 2 calima MediumCIM-RM00-120-3 957 1 1 calima MediumCIM RM00 30 967 4 1 calima LargeCIM-RM00-30 967 4 1 calima LargeCIM-RM02-16-2 968 1 2 calima MediumCIM-RM00-93-6 990 1 1 calima MediumCIM-RM00-9 991 1 2 calima LargeCIM RM02 30 1 994 4 1 calima LargeCIM-RM02-30-1 994 4 1 calima LargeCIM-RM00-201-1 1027 1 1 calima LargeSUG 131 1030 1 1 sugar LargeCIM-RM00-191-2 1034 1 1 calima LargeCIM-RM00-321-1 1605 1 1 calima MediumCIM-RM00-321-1 1605 1 1 calima MediumKABALABALA 1615 1 1 calima SmallCIM-RM02-25-1 1677 1 1 calima LargeCIM-RM01-55-4 1728 1 1 calima LargeMean 1191 1 1Mean 1191 1 1CV% 15 41 32SE +/_ 143.7 0.3 0.241

Beans for Low Soil Fertility Stress Genotype Yield-kg ha-1 Rust CBB Colour SizeGenotype Yield kg ha Rust CBB Colour SizeRA 13132-2-1-2 309 7 1 Red SmallCIM-RM00-290-03 322 1 1 Segreg MedVTTT 917/6-4-3 388 1 1 Red LargeFEB 196 434 1 1 Khaki SmallFEB 196 434 1 1 Khaki SmallVTTT 924/15-2 542 1 2 Sug LargeMN 13389-6 584 2 1 Black SmallRA 13182 587 6 1 Red SmallMORE 92018 590 1 1 Khaki SmallMORE 92018 590 1 1 Khaki SmallCIM-RM00-321-2 609 1 2 Calima LargeCIM-RM00-323-3 748 2 1 Calima LargeCAL143 752 2 1 Calima MediumBF 13572-6 983 3 1 Khaki Small357 6 983 3 SUBR[92]25 988 1 1 Navy SmallVTTT 923/9-5-3 990 1 1 Segreg LargeMN13509-8-1 1012 1 1 Black SmallBF 13573-6 1373 1 1 Red SmallMR 13456-12-13 1435 1 1 Red SmallVTTT 924/2-4-2-1 1449 1 1 Sugar LargeBF 13572-7 1561 1 1 Red SmallMN13509-8-6 1571 1 1 Black SmallMean 879 1 1CV% 22 32 32SE +/_ 141.8 0.30 0.26

Beans Response to P and Rhizobium Genotype

Yield-kg ha-1 Colour size+ P + Rz -P - RzGenotype P Rz P Rz

SER 72 2805 1004 Red SmallTIOCANERA 75 2555 1867 Red SmallBF 13607-9 2510 1284 Khaki SmallS 2320 1611 R d S llSER 55 2320 1611 Red SmallMR 13456 2264 2015 Red SmallSER 85 1847 1736 Red SmallSER 75 1800 1324 Red SmallSER 75 1800 1324PC652-553/VAX5 1670 896 Sugar SmallSER 81 1649 1067 Red SmallSER 124 1503 1346 Red Small

R d S llBF 13572-5 1489 1520 Red SmallSER 78 1455 1368 Red SmallSXB 405 1442 2035 Khaki SmallBF 13607-12 996 1193 Carioca SmallBF 13607 12 996 1193 Carioca SmallCAL143/SUG131 630 1186 Calima LargeCAL 143 (LOCAL) 626 685 Calima LargeVTTT925/9-1-2 589 676 Red LargeMean 1681 1339SE +/_ 420.4 300.8CV% 43.3 38.9

Bean variety Response to P and Rhizobium

3000

2000

2500

1000

1500

Yiel

d K

g/ha

TreatmentControl

500

1000

0

SER 72

SER 85

SXB 41

3SER

83BF 1

3572

-5

PC652-5

53/V

AX5SER

53

CAL 143

(LOCAL)

SER 75

VTTT9

24/19

-8-1

AL 143

/SUGAR

131

SER 78

TIOCANERA 75

P CA

CAL

Genotype

Climbing Beans Genotype Rust CBB Yield kg ha-1 Colour SizeCIM-CLIMB-01-03-48 1 1 1089 Calima LargeCIM-CLIMB-01-03-48 1 1 1089 Calima LargeCIM-CLIMB-01-01-08 1 1 931 Calima MediumCIM-CLIMB-01-04-42 1 1 915 Segregating SmallCIM-CLIMB-01-04-23 1 1 901 Segregating MediumCIM-CLIMB-01-05-01 1 1 899 Calima SmallCIM-CLIMB-01-05-01 1 1 899 Calima SmallCIM-CLIMB-01-04-18 1 1 856 Calima MediumCIM-CLIMB-01-02-02 2 1 847 Red SmallCIM-CLIMB-01-03-31 2 1 839 Calima MediumCIM-CLIMB-01-04-15 1 2 838 Calima LargeCIM CLIMB 01 04 15 1 2 838 Calima LargeCIM-CLIMB-01-03-27 3 1 833 Calima MediumCIM-CLIMB-01-04-12 1 1 811 Calima MediumCIM-CLIMB-01-04-21 1 1 807 Calima MediumCIM-CLIMB-01-01-01 1 1 798 Segregating MediumCIM CLIMB 01 01 01 1 1 798 Segregating MediumCIM-CLIMB-01-04-36 1 1 786 Pink SmallCIM-CLIMB-01-04-34 1 1 784 Segregating SmallCIM-CLIMB-01-04-35 1 1 783 Calima LargeCIM-CLIMB-01-03-18 2 1 770 Mwezimoja LargeCIM CLIMB 01 03 18 2 1 770 Mwezimoja LargeCIM-CLIMB-01-01-06 1 1 746 Calima LargeCIM-CLIMB-01-04-17 1 1 746 Calima MediumCIM-CLIMB-01-01-12 1 2 742 Segregating LargeKANZAMA 1 1 637 red largeKANZAMA 1 1 637 red largeMean 2 1 620SE +/_ 0.43 0.24 129.4CV% 35.2 21.3 26.6

Breeder Seed Increase

Variety Quantity Variety QuantityVariety Quantity (kg)

Variety Quantity (kg)

Napilira 350 Maluwa 335pKholophethe 395 Nagaga 68Mkhalira 132 Sapatsika 33K bid i 63 NUA 59 9Kambidzi 63 NUA 59 9NUA 45 7.5 VTTT 924/4-4 13MAC 51 22 MAC 53 11

Network SupportOutcome #1: Access to bean varieties resistant to multiple

stressesOutcome #2: Farmers access integrated stress management

optionsOutcome #3: Improved nutrition and health for the vulnerableOutcome #3: Improved nutrition and health for the vulnerable

communities (children, women, and PLWHA)Outcome #4: Stakeholders benefit more from market opportunitiesOutcome #5: Reaching all users with bean-based technologies,

processes and informationOutcome #6: PABRA partners have increased ability to respond toOutcome #6: PABRA partners have increased ability to respond to

demands in the bean sector, and are monitoring change

O t #7 I d i t ti f d d litOutcome #7: Increased integration of gender and equality perspectives in implementation of PABRA research and development agenda

2009‐10 WORK PLANS AND BUDGETSCountry Stress

VarStress

Non-VarNutrition Markets REU M&E Gender TOTAL

AO 4,900 925 900 1,000 8,500 16,225, , , ,CD 3,900 925 900 4,100 3,000 3,000 15,825LS 3,900 450 900 1,000 6,000 12,250MW 6 730 925 9 200 4 250 600 1 000 22 705MW 6,730 925 9,200 4,250 600 1,000 22,705MR 2,350 2,500 4,850MZ 3,900 925 900 3,100 8,825SZ 3,900 925 900 3,200 11,800 20,725TZ 6,230 925 11,450 5,650 2,500 1,000 27,755ZA 7 880 450 2 350 4 800 7 500 22 980ZA 7,880 450 2,350 4,800 7,500 22,980ZM 6,230 925 11,050 6,150 9,200 3,000 36,555ZW 5,230 925 2,950 5,250 8,500 22,855TOTAL 55,150 8,300 41,500 38,500 60,100 8,000 211,550

1st Disbursement of Funds – Nov 2009

2009‐10 WORK PLANS AND BUDGETSCountry Stress

VarStress

Non-VarNutrition Markets REU M&E Gender TOTAL

AO 1 000 2 000 3 500 1 000 1 500 9 0001,000 2,000 3,500 1,000 1,500 9,000CD 1,000 2,000 2,500 1,000 7,500 7,500 21,500LS 250 1,500 2,200 1,000 4,950MWMW 4,500 3,000 4,000 1,750 4,000 500 17,750MR 250 1,000 1,200 1,500 3,950MZ 2,500 3,000 2,500 1,500 9,500,500 3,000 ,500 ,500 9,500SZ 250 2,000 1,500 2,200 1,000 1,500 8,450TZ 4,500 3,000 4,000 1,750 4,000 500 17,750ZAZA 4,000 3,000 1,750 9,000 10,500 28,250ZM 3,500 3,000 5,000 4,750 6,000 4,500 26,750ZW 1,750 3,000 4,000 4,750 6,000 1,500 21,000TOTAL 23,500 21,000 32,500 22,350 37,500 32,000 168,850

2nd Disbursement of Funds – Dec 2009

Other Network Support to NARS ‐20102010

Activity CountriesH i ti f th R i l S d S t M l i S il dHarmonization of the Regional Seed Systems Project has been funded by SDC and FARNPAN will host the project covering 4

Malawi, SwazilandZambia, Zimbabwe

countriesAnother concept note (HASSP +5) to cover 5 more countries was developed by the CGIAR

Mozambique,  Angola, Botswana,

centres and submitted to USAID Lesotho, Namibia 

Develop a concept Note for Phase II of the Malawi, Tanzania, p pBean Seed Systems project funded by McKnight Foundation

, ,Mozambique

Infrastructure Developmenth h d l d h f ll• Through GCP and TL‐I, Malawi received the following equipment for marker assisted selection:

Liophilizer– Liophilizer, – Gel tray and power supply system, – Micro‐pipettes,Micro pipettes, – PCR machine and sensitive balances.

• Double cab pick‐up vehicles for Zambia and Angolap p g• Laptop computers for Lesotho, Swaziland and Tanzania• A printer and camera for LesothoA printer and camera for Lesotho• Plant pathology laboratory supplies for Angola, Malawi, South Africa, Tanzania, Zambia and Zimbabwea a , Sout ca, a a a, a b a a d bab e

Bean Germplasm Distributed in 2009 – 10Nursery Entries COUNTRY

AO CD LS MW MZ SZ TZ ZM MUSARBYT 20 1 3 3 3 1 1 3SARBEN 100 1 3 3Drought small 36 1 1 1 1 1BSM 29 1 1 3 2 2S 16 1 1 1 3 1 1 1Sugar nursery 16 1 1 1 3 1 1 1ALS nursery 45 1 1 2 2 2NAVY 10 1 1 2 1 2BIOFORT-FT 31 1 1 2 1 1 1 1Medium climbers 15 1 1 1 1 1SARBEN climber 81 1 2 1 1 1CALIMA LOW N 17 3 2 2NEW BILFA 75 1 1 1 3 2 1Farmers nursery Low P 6 1 2 2 1CREAM dwarf/climber 4 3 1CREAM dwarf/climber 4 3 1COMMERCIAL varieties 16 3 1 1PVS varieties 18 1 1 3 1 2Farmers nursery Low N 4 2 1 2High mineral nursery 72 1 3 1 1 1 1 1GCI DRY BEANS SET I 18 1 1 1 1GCI DRY BEANS II 6 1 1 1RILS-05A 21 1 1 3 1 1MWEZIMOJA D/F 11 1 2CALIMA dwarf/climber 18 1 1CALIMA dwarf/climber 18 1 1CALIMA nursery 76 1 1 1 1 1KHAKI nursery 25 1 1Mozambique lines 6 6 1

Available Germplasm for 2010 – 11# f # f Nursery # of entries

Nursery # of entries

KHAKI NURSERY SET 2 18 SARBEN 2 75CALIMA NURSERY SET 2 83 DROUGHT SMALL SEEDED 36CALIMA NURSERY SET 2 83 DROUGHT SMALL SEEDED 36RED KIDNEY SET 2 17 KHAKI 25COMMERCIAL VARIETIES 16 CALIMA 81GCI DRY BEANS SET 2 6 NEW BILFA 64GCI DRY BEANS SET 2 6 NEW BILFA 64GCI DRY BEANS SET 1 18 ALS NURSERY 54PURPLE DC NURSERY 11 SARBYT 20NAVY NURSERY 10 HIGH MINERAL 72BSM NURSERY 29 BMZ DROUGHT TRIAL 26BIOFORT FAST TRACK 31 MALAWI PVS TRIAL 20RILS -05A NURSERY 21 MCR NURSERY 440SUGAR NURSERY 16 NUVS NURSERY 64FARMERS NURSERY LOW N 4 MID ALTITUDE CLIMBERS 12CALIMA NURSERY LOW N 17 MCK CLIMBERS SET-1 17FARMERS NURSERY LOW P 6 MCKNIGHT CLIMBERS SEL 10FARMERS NURSERY LOW P 6 MCKNIGHT CLIMBERS - SEL 10CREAM D-C NURSERY 4 DEMONSTRATION CLIMBERS 7SARBEN 1 100 SARBEN CLIMBERS 68

Network Coordination Visits ‐2010Country Date ActivitySouth AfricaMarch 2010 Visit trials at Cedara and on‐farm with 

PABRA SC stakeholdersSwazialnd March 2010 Visit trials on‐station and on‐farm, plus 

meetings with the Ministry ofmeetings with the Ministry of Agriculture officials

South AfricaApril 2010 Follow‐up on MSc student thesis hresearch progress

Lesotho April 2010 Follow up on SABRN activities and deliver capital equipment for Lesothop q p

Zimbabwe April 2010 Visit the national bean research team and trials ‐ Harare, Zimbabwe

Tanzania July 2010 Visit ARI Uyole with NRI people toTanzania July 2010 Visit  ARI‐Uyole, with NRI people to initiatiate the botanical pesticide project

Degree TrainingDegree Training

NAME DEG UNIVERSITY FUNDING STATUSSimon Bereng MSc Univ of Tasmania Other 1st YearAb h Dhl i i MS U i f P t i Oth 1st YAbraham Dhlamini MSc Univ of Pretoria Other 1st YearVirginia Chisale MSc Penn State Univ. USAID 2nd YearGodwill Makunde PhD Free State Univ. TL-II 2nd Year

Isaac Fandika PhD New Zealand Other 2nd YearMagalhaes Miguel PhD Penn State Univ. McKnight Last YearS X i d PhD P St t U i M K i ht L t YSoares Xerinda PhD Penn State Univ. McKnight Last YearCelestina Jochua PhD Penn State Univ. McKnight Last YearLizzie Kalolokesya MSc Univ. of Zambia TL-II Finished

NARS Participation in Training and k hWorkshops

• Plant Pathology Short Training Course: This was conducted at CIAT‐Uganda, and participants included: Angola, Malawi, South Africa, Tanzania, Zambia and Zimbabwe T i i IPHIS l d t b t• Training on IPHIS – a germplasm database system was conducted in Nairobi, Kenya and participants included: Malawi, Zimbabwe, Tanzania, ,

• Markets workshop – February 2010• M & E Workshop, Uganda – June 2010p, g• DIVA workshop, Uganda – August 2010 • Stakeholder’s seed workshop for partners in DR Congo, 

Zambia and Zimbabwe – held in Lusaka, August –September 2010

Network Representation S k h ld ’ S d W k h f i S h Af i h ld• Stakeholders’ Seed Workshop for partners in South Africa – held at Potchefstroom, February 2010

• Participated in ECABREN SC & gender workshop – February 2010Participated in ECABREN SC & gender workshop  February 2010• PABRA SC meeting at Cedara, South Africa – March,  2010• Participated in the CIAT annual Review meeting – May 2010• Participate in the HASSP inception workshop, Johannesburg –

May 2010P ti i t d i TL I A l R i ti i S i A t• Participated in TL‐I, Annual Review meeting in Spain – August 2010 

• Organized the Bean Seed Systems Workshop – held at CrossOrganized the Bean Seed Systems Workshop  held at Cross Roads Hotel, Malawi – September 2010

• Participated in the CCRP East and Southern Africa Culture of i i i hi S b 2010Practice meeting in Mangochi – September 2010