Sabrina Rose Leslie

Contact informationRutherford Physics 214 sabrina.leslie@mcgill.caMcGill University +1 (514) 882-36493600 rue University LeslieLab: http://www.physics.mcgill.ca/leslielab/index.htmlMontreal, QC, Canada H3A 2T8 ScopeSys: http://www.scopesys.ca

Employment/Education

Jan 2018 – Visiting Scientist (Sabbatical)Dec 2018 UBC, Pharmaceutical Science

Stanford, Bioengineering

June 2017 – Associate ProfessorPresent Department of Physics & Quantitative Life Sciences Program

McGill University

Jan. 2012 – Assistant ProfessorMay 2017 Department of Physics, McGill University

Mar. 2009 – Marie Fieser Postdoctoral fellow (Adam Cohen Biophysics Group)Dec. 2011 Department of Chemistry and Chemical Biology, Harvard University

Sept. 2002 – PhD in Physics (Stamper-Kurn Cold Atoms Group)Dec. 2008 Dept. of Physics, University of California at Berkeley (UCB),

Berkeley, California, USA (PhD Thesis.pdf)Sept. 1998 – BSc (Hon.) in Physics and MathematicsApr. 2002 Dept. of Physics and Astronomy, University of British Columbia,

Synopsis: My academic training began in 1998 as an undergraduate at UBC, where Iimmersed myself in the Combined Honours Physics and Mathematics Program, graduatingas a Canada Scholar and recipient of the CK Choi Presidential Award. In 2002, I moved toUC Berkeley as an NSERC fellow to pursue doctoral studies in optical and atomic physics.Under the guidance of Dr. Dan Stamper-Kurn, I pioneered imaging experiments of disorder-to-order transitions of atomic vapours held in optical traps. My PhD work was publishedin top journals and provided me with extensive training in instrumentation, imaging, dataanalysis, and team-based science. In 2009, I expanded my horizons, shifting from visualizingatoms in vacuum chambers, to visualizing biomolecules in liquids; a transition inspired bya desire to get a little closer to life in my research. This transition to the Department ofChemistry and Chemical Biology at Harvard University was made possible with a MaryFieser postdoctoral fellowship, with the mandate to explore this inspiration. At HarvardI invented a single-molecule imaging technology called Convex Lens-induced Confinement(CLiC), which established me as a pioneer in single-molecule investigations with a rangeof applications. In 2012, I became an Assistant Professor at McGill University and myresearch group has developed CLiC into a platform technology and used it for new studiesof nucleic acids, proteins, polymers, nanomaterials, cells, with applications to therapeuticsand genomics. My time at McGill has been productive and I was promoted to AssociateProfessor with tenure in 2017. To further expand on the CLiC imaging platform and lead itsapplication to real-world problems, I co-founded a start-up company ScopeSys.

1

1 Research Contributions

My current research program pioneers new measurements of the interactions and dynam-ics of macromolecules, with four principal contributions detailed in the publications below:

1.) Invented a new single-molecule technique called Convex Lens-induced Con-finement, and pioneered its development into a new platform for precision single-molecule biophysics and biotechnology. CLiC enables solution-based measurements of untethered molecules in cell-like conditions, with high statistics and single-molecule reso-lution, with key applications in therapeutics, medicine, and nanoscience [1,5,12,17,19]. We have also extended CLiC to address questions from the scale of molecules to living cells [11,2].

2.) Lead research to advance our understanding of structure-activity rela-tionships which play a key role in governing molecular interactions. With cancer scientist Dr. David Levens at the NIH, we study DNA-DNA and DNA-protein interactions with new resolution [6,9]. We have made surprising observations of the interplay between crowding and ionic strength in regulating DNA structural transitions, and their influence on binding interactions [3]. These discoveries impact a wide range of in vitro biochemistry experiments: they indicate that crowded and confined environments inside the cell, as well as dynamic higher-order structure of macromolecules, actively influence interactions [4].

3.) Lead research to advance our fundamental understanding of confined poly-mers. In collaboration with theorists, we investigate the behavior of DNA polymers under confinement, from the nano to microscale regimes, in one device [10]. By achieving a detailed understanding of DNA conformations and dynamics as a function of confinement, and pro-viding new data which enables theories from different regimes to be continuously connected, we can inform the development of biotechnologies which load and handle macromolecules [8].

4.) Lead research to advance genomics studies by uniformly extending DNA in nanogrooves. The CLiC high-throughput approach can be used to array DNA in nanogrooves, with uniform extension, to facilitate mapping DNA sequence to position [13,14,16], thus enabling single-molecule sensitivity in a wide range of “omics” analyses. We have been innovating and been integrating nanofluidics with our CLiC technology to advance long-read sequencing and biomarker sensing applications in a scalable, miniature format [5].

2 Publication List

The peer-reviewed manuscripts by my McGill team are dated after 2012 and the names of trainees whom I have supervised at McGill are underlined.

2.1 Publications with Peer Review

[1] Sabrina Leslie∗ Single-molecule imaging of the biophysics of molecular interactionswith precision and control, in cell-like conditions, and without tethers. (Invited review).Current Opinion in Biomedical Engineering, in press November 2019.

2

[2] K. Thiombane, N. Coutin, D. J. Berard, R. Tahvildari, Sabrina Leslie∗, C. Nislow.Single-cell analysis for drug development using CLiC imaging.Biotechniques, in press November 2019.

[3] Shane Scott, Cynthia Shaheen, Brendon McGuiness, Kimberly Metera, Fedor Kouzine,David Levens, Craig J Benham, Sabrina Leslie∗.Single-molecule visualization of the effects of ionic strength and crowding on structure-mediated interactions in supercoiled DNA molecules.Nucleic Acids Research, 0305-1048 (2019).

[4] Marjan Shayegan, Radin Tahvildari, Lydia Kisley, Kimberly Metera, Stephen W.Michnick Sabrina Leslie∗.Probing inhomogeneous diffusion in the microenvironments of phase-separated polymersunder confinement.JACS 141(19),197751-7757 (2019)

[5] Daniel Berard, Sabrina Leslie∗.Miniaturized flow cell with pneumatically-actuated vertical nanoconfinement for single-molecule imaging and manipulation.Biomicrofluidics 12, 054107 (2018)Recipient of Top Poster Prize at the Canadian Biophysical Society Meeting, June 2018

[6] Shane Scott, Zhi Ming Xu, Fedor Kouzine, Daniel J. Berard, Cynthia Shaheen,LauraSaunders, Barbara Gravel, Alexander Hofkirchner, Catherine LeRoux, Jill Laurin,David Levens, Craig Benham, Sabrina R. Leslie∗.Visualizing structure-mediated interactions in supercoiled DNA molecules.Nucleic Acids Research 46, 4622-431 (2018)Recipient of Top Poster Prize at UBC Nanomedicine Day Conference, Sept 2018

[7] Sabrina R. Leslie∗, Albert Kamanzi, Daniel Berard, Marjan Shayegan, Gilead Henkin,Jason Leith, Shane Scott, Francis Stabile.Biological Confinement Physics: Squeezing New Information out of Complex Macro-molecules. Invited Review Article, Physics in Canada, Special Issue (2017).

[8] Tyler Shendruck∗, Dave Sean∗, Daniel Berard∗, Julian Wolf, Justin Dragoman, SophieBattat, Gary Slater, Sabrina R. Leslie∗.Rotation-induced macromolecular spooling of DNAPhysical Review X, 7(3),031005 (2017)

[9] Gilead Henkin, Daniel Berard, Francis Stable, Marjan Shayegan, Jason S. Leith, Sab-rina R. Leslie∗.Manipulating and visualizing molecular interactions in customized nanoscale spaces.Analytical Chemistry 88(22), 1110011107 (2016)

[10] Jason S. Leith∗, Albert Kamanzi∗, Dave Sean, Daniel Berard, Andrew Guthrie, ChristopherM.J. McFaul, Gary Slater, Hendrick de Haan∗, Sabrina R. Leslie∗.Free Energy of a Polymer in Slit-like Confinement from the Odijk Regime to the Bulk.Macromolecules 49(23), 92669271 (2016)

3

[11] Bojing Jia, Tse-Luen Wee, Daniel J. Berard, Adiel Mallik, David Juncker, Claire M.Brown∗, Sabrina R. Leslie∗

Parallelized Cytoindentation Using Convex Micropatterned Surfaces.Biotechniques 61, No. 2, 73-82 (2016)

[12] Daniel Berard∗, Marjan Shayegan∗, Francois Michaud, Gilead Henkin, Shane Scott,Sabrina R. Leslie∗.Formatting and Ligating Biopolymers using Adjustable Nanotopographies.Applied Physics Letters 109, 033702-033706 (2016)

[13] Jalal Ahamed, Sara Mahshid, Daniel Berard, Francois Michaud, Rob Sladek, WalterReisner∗, Sabrina R. Leslie∗.Continuous Confinement Fluidics: Getting Lots of Molecules in Small Spaces.Macromolecules 49, (7) 2853-2859 (2016).

[14] Sara Mahshid, Mohammed Jalal Ahamed, Daniel Berard, Susan Amin, Rob Sladek,Sabrina R. Leslie∗, Walter Reisner∗

Development of a Platform for Single-Cell Genomics Using Convex Lens-Induced Con-finement.Lab on a Chip 15, 3013-3020 (2015).

[15] Adriel Arsenault, Jason Leith, Gil Henkin, Christopher McFaul, Matthew Tarling, R.Talbot, Daniel Berard, Francois Michaud,Shane Scott, Sabrina Leslie*.Open-frame System for Single–Molecule Microscopy.Rev. Sci. Instrum. 86(3), 033701 (2015).

[16] Daniel Berard, Francois Michaud, Sara Mahshid, Mohammed Jalal Ahamed, ChristopherMcFaul, Jason Leith, Pierre Berube, Rob Sladek, Walter Reisner∗, Sabrina R. Leslie∗

Convex lens-induced nanoscale templatingP.N.A.S. 111, 37 (2014).Featured by PNAS Cover commentary, Genome Web, and other media.

[17] Daniel Berard, Christopher McFaul, Jason Leith, Adriel Arsenault, Francois Michaud,Sabrina Leslie*.Precision Platform for Convex Lens-Induced Confinement Microscopy.Rev. Sci. Instrum. 84, 103704 (2013).Featured as editor’s pick on the front page of Rev. Sci. webpage.

[18] Mary Williard Elting, Sabrina R. Leslie, L. Stirling Churchman, Jonas Korlach,Christopher McFaul, Jason S. Leith, Michael J. Levene, Adam E. Cohen, James A.SpudichSingle- molecule fluorescence imaging of processive myosin with enhanced backgroundsuppression using linear Zero Mode Waveguides (ZMW) and Convex Lens-induced Con-finement (CLiC)Optics Express 21 (1), 1189-1202 (2013).

[19] Sabrina R. Leslie, Alexander P. Fields, Adam E. Cohen.Convex Lens-induced Confinement for Imaging Single Molecules.Analytical Chemistry 82 (14), 6224-6229 (2010).Featured in Technology Review and C&E News.

4

[20] Adam E. Cohen, Alexander P. Fields, Jennifer H. Hou, Sabrina R. Leslie, Min J.Shon.In honor of W. E. Moerner: Confining molecules for single-molecule spectroscopy.Isreal Journal of Chemistry (IJC) 49 (3-4), 275 (2010).

[21] Jay D. Sau, S.R. Leslie, Marvin L. Cohen, D.M. Stamper-Kurn.Spin squeezing of high-spin, spatially extended quantum fields.New J. Phys. 12, 085011 (2010).

[22] M. Vengalattore, J. Guzman, S. R. Leslie, F. Serwane, and D. M. Stamper-Kurn.Periodic spin textures in a degenerate F=1 87Rb spinor Bose gas.Physical Review A 81, 053612 (2010).Featured in Science News: “Evidence mounts for an exotic supersolid”.

[23] S.R.Leslie, J.Guzman, M.Vengalattore, J.D. Sau, M.L. Cohen, D.M. Stamper-Kurn.Amplification of fluctuations in a spinor Bose Einstein condensate.Physical Review A 79, 043631 (2009).Featured in PRA’s Kaleidoscope.

[24] J.D. Sau, S.R.Leslie, D.M. Stamper-Kurn, M.L. Cohen.Theory of domain formation in inhomogeneous ferromagnetic dipolar condensatesPhysical Review A 80, 023622 (2009).Featured in PRA’s Kaleidoscope.

[25] M.Vengalattore, S.R.Leslie, J.Guzman, D.M. Stamper-Kurn.Spontaneously modulated spin textures in a dipolar spinor Bose-Einstein condensate.Physical Review Letters 100, 170403 (2008).

[26] M. Vengalattore, J. M. Higbie, S. R. Leslie, J. Guzman, L. E. Sadler, D. M. Stamper-Kurn.High-resolution magnetometry with a spinor Bose-Einstein condensate.Physical Review Letters 98, 200801 (2007).Featured in Nature research highlights: “Best served chilled”.

[27] L. E. Sadler, J. M. Higbie, S. R. Leslie, M. Vengalattore, D. M. Stamper-Kurn.Coherence-enhanced imaging of a degenerate Bose gas.Physical Review Letters 98, 110401 (2007).

[28] L. E. Sadler, J. M. Higbie, S. R. Leslie, M. Vengalattore, D. M. Stamper-Kurn.Spontaneous symmetry breaking in a quenched ferromagnetic spinor Bose condensate.Nature 443, 312 (2006).

[29] J. M. Higbie, L. E. Sadler, S. Inouye, A. P. Chikkatur, S. R. Leslie, K. L. Moore, V.Savalli, D. M. Stamper-Kurn.Direct, non-destructive imaging of magnetization in a spin-1 Bose gas.Physical Review Letters 95, 050401 (2005).

[30] K. L. Moore, T. P. Purdy, K. W. Murch, S. Leslie, S. Gupta, and D. M. Stamper-Kurn.

5

Collimated, single-pass atom source from a pulsed alkali metal dispenser for laser-cooling experiments.Rev. Sci. Instrum. 76, 023106 (2005).

[31] S. Leslie, N. Shenvi, K. R. Brown, Dan M. Stamper-Kurn, and K. Birgitta Whaley.Transmission spectrum of an optical cavity containing N atoms.Phys. Rev. A 69, 043805 (2004).

2.2 Non-refereed publications while at McGill

[1] Future-Ready: McGill’s Sabrina LeslieResearch and Innovation, McGill University (2019).

[2] Sabrina R. Leslie.CLiC to Enhance - Molecular imaging for the normal lab: a simple, cost-effective al-ternative.The Pathologist 0214 (2014).

[3] Sabrina R. Leslie, Daniel Berard, Jason S. Leith, Francois Michaud.Using Tunable Nanoscale Confinement to Image and Manipulate DNA.OSA Conference Proceeding. Optical Sensors: Micro and Nano-Engineered Sensors,Barcelona, Spain. (2014).

[4] Christopher M.J. McFaul, Jason S. Leith, Bojing Jia, Francois Michaud, Adriel Arsenault,Andrew Martin, Daniel Berard, Sabrina R. Leslie. Single-Molecule Microscopy UsingTunable Nanoscale Confinement.SPIE Conference Proceeding, 8811 (2013).

3 Invited seminars, lectures and colloquia

I have given over 70 presentations, most of them invited, at conferences and universities (not including industry talks). I have recently emphasized invited international talks.

3.1 Invited presentations at conferences

1. May 21, 2020: Precision single-molecule biochemistry using applied nanoscale physics.Young Investigator Award Talk, Canadian Biophysical Society Meeting, Dalhousie, Canada(Upcoming).

2. August 25-29, 2019: Deconstructing biology with simple single-molecule imaging:Controlling conformation, confinement, and concentration.ACS Meeting, Symposium on “Confined dynamics of molecules and particles at inter-faces, in pores, and under crowded conditions, San Diego, USA.

3. June 4-5, 2019: Deconstructing biology with simple single-molecule imaging: Control-ling conformation, confinement, and concentration.Swedish Microfluidics in Life Science Conference, Chalmers, Sweden.

6

4. May 28-30, 2019: Deconstructing biology with simple single-molecule imaging: Con-trolling conformation, confinement, and concentration.Canadian Biophysical Society - University of Toronto, Canada.

5. May 3-5, 2019: Deconstructing biology with simple single-molecule imaging: Control-ling conformation, confinement, and concentration.Keynote talk for 18th Chemical Biophysics Symposium - University of Toronto, Canada.

6. March 31 - April 4, 2019: Deconstructing biology with simple single-molecule imag-ing: Controlling conformation, confinement, and concentration.ACS Meeting, Symposium on “Frontiers in Fluorescence Microscopy”. Orlando, Florida.

7. March 2 - 6, 2019: Deconstructing biology with simple single-molecule imaging: Con-trolling conformation, confinement, and concentration.American Biophysical Society Annual Meeting. Baltimore, USA.

8. 15-20 July 2018: Single-molecule visualization of structure-mediated interactions insupercoiled DNA.Gordon Research Conference, Single-molecule approaches to Biology. Vermont, USA.

9. 26-30 June 2018: Single-molecule visualization of structure-mediated interactions insupercoiled DNA.Telluride Workshop: Theory Meets Experiment. Telluride, CO, USA.

10. 26-30 May 2018: Single-molecule visualization of molecular interactions.Columbia Workshop: Molecules, Materials, Devices and Systems in Medicine. ColumbiaUniversity, New York City, USA.

11. 5-9 March 2018: Single-molecule visualization of structure-mediated interactions insupercoiled DNA.APS March meeting, Recent Advances in Single Polymer Dynamics. Los Angeles,California, USA.

12. 21-24 February 2018: How biomolecules behave in a squeeze.DNA and Interacting Proteins as Single Molecules, In Vitro and In Vivo conference,Fiesta Americana Condesa, Cancun, Mexico.

13. 20-24 June 2017: Single-molecule visualization of topology-mediated biomolecular in-teractions, using nanoconfinement microscopy.The Complexity of Dynamics and Kinetics from Single Molecules to Cells. Telluride,Colorado, USA.

14. 10 May 2017: Single-molecule visualization of topology-mediated biomolecular inter-actions, using nanoconfinement microscopy.Canadian Microscopy and Cytometry Symposium on Micro/Nanofluidics for OpticalMicroscopy. Montreal, Quebec, Canada.

15. 10 November 2016: Squeezing new information out of DNA using tunable nanoto-pographies.Nano Ontario Conference, Guelph, Ontario.

7

16. 9 September 2016: 1.) Getting into that room at the bottom: formatting DNAusing tunable nanoscale confinement. 2.) How DNA do the twist: visualizing supercoil-induced site-unwinding and site-invasion in DNA loops.13th Greta Pifat International School of Biophysics 2016, Croatia.

17. 1 June 2016: Squeezing new information out of DNA using tunable nanotopographies.Canadian Biophysics Society, University of Manitoba, MB, Canada.

18. 26 May 2016: Squeezing new information out of DNA using tunable nanotopographies.Next Generation Sequencing GTC Bio Meeting, Boston, MA, USA.

19. 18 June 2015: Getting into that Room at the Bottom of DNA analysis using tunablenanoscale confinement.CAP Congress, University of Alberta, AB, Canada.

20. 16 June 2015: Getting into that Room at the Bottom of DNA analysis using tunablenanoscale confinement.Symposium on Advances in Biological Imaging and Spectroscopy, Canadian ChemistryConference, Ottawa, Canada.

21. 29 May 2015: Getting into that Room at the Bottom of DNA Analysis using tunablenanoscale confinement.Symposium on Fluorescence Microscopy at the Nanoscale, Canadian Microscopy Con-ference, McMaster University, Canada.

22. 25 August 2013: Single-molecule microscopy using tunable nanoscale confinementSPIE OP106 2013 - Physical Chemistry of Interfaces and Nanomaterials XII Confer-ence, San Diego, USA.

23. 31 May 2013: Precision spectroscopy of DNA under physiologically compelling condi-tionsCAP Congress, Montreal, Canada.

3.2 Invited seminars and colloquia

1. 30 January 2020: Precision single-molecule biochemistry using applied nanoscalephysics: watching many single molecules interact, without tethers and yet with control.Biophysics seminar, Chalmers Sweden (Upcoming).

2. 21 November 2019: Deconstructing biology with simple single-molecule imaging:Controlling conformation, confinement, and concentrationBiological Physics Seminar, Arizona State University, USA (Upcoming).

3. 18 October 2019: Deconstructing biology with simple single-molecule imaging: Con-trolling conformation, confinement, and concentrationCenter for NanoScience & Physics Dept, LMU, Munich, Germany (Upcoming).

4. 7 March 2019: Deconstructing biology with simple single-molecule imaging: Control-ling conformation, confinement, and concentrationDepartment of Physics, UBC, Vancouver, Canada.

8

5. 8 & 15 Jan 2019: Deconstructing biology with simple single-molecule imaging: Con-trolling conformation, confinement, and concentrationQuantitative Life Sciences & Chemistry Dept, McGill University, Montreal, Canada.

6. 10 Dec 2018: Deconstructing biology with simple single-molecule imaging: Controllingconformation, confinement, and concentrationYale University, New Haven, USA.

7. 23 Oct 2018: Deconstructing biology with simple single-molecule imaging: Controllingconformation, confinement, and concentrationStanford University, Stanford, USA.

8. 30 April 2018: Visualizing and understanding molecular interactionsBC Cancer Agency, Vancouver, Canada.

9. 14 February 2018: Visualizing and understanding molecular interactionsUBC, Department of Pharmaceutical Sciences, Vancouver, Canada.

10. 4 February 2018: Visualizing and understanding molecular interactionsSimon Fraser University, Biophysics Seminar, Vancouver, Canada.

11. 15 December 2017: How biomolecules behave in a squeeze.UIUC, Biophysics Seminar, Illinois, USA.

12. 20 November 2017: How biomolecules behave in a squeeze.UBC, Department of Chemistry, Vancouver, Canada.

13. 27 October 2017: How biomolecules behave in a squeeze.McGill, Department of Physics, Montreal, Canada.

14. 18 October 2017: How biomolecules behave in a squeeze.Cornell University, Biophysics Seminar, Ithaca, New York, USA.

15. 8 September 2017: Single-molecule visualization of topology-mediated interactions insupercoiled DNA.Colorado State University, Department of Chemical and Biological Engineering, Col-orado, USA.

16. 21 April 2017: Confinement microscopy of bionano materials.Mirexus Inc.and Guelph University, Guelph, Ontario, Canada.

17. 14 November 2016: How DNA do the twist: visualizing supercoil-induced site-unwinding and site-invasion in DNA loops.U.C. Davis, CA, USA.

18. 21 October 2016: Squeezing new information out of DNA using tunable nanotopogra-phies.Oxford University, Oxford, England.

19. 20 October 2016: Squeezing new information out of DNA using tunable nanotopogra-phies.Marie Curie Institute, Paris, France.

9

20. 19 October 2016: How DNA do the twist: visualizing supercoil-induced site-unwindingand site-invasion in DNA loops.Universite Paris Diderot, Paris, France.

21. 18 October 2016: Squeezing new information out of DNA using tunable nanotopogra-phies.Universite d’Evry val d’Essonne, France.

22. 27 May 2016: Squeezing new information out of DNA using tunable nanotopographiesNorthEastern University, Department of Physics, Massachusetts, USA.

23. 19 April 2016: Squeezing new information out of DNA using tunable nanotopographiesUniversity of Rochester, Department of Biomedical Engineering and Optics, New York,USA.

24. 29 January 2016: Squeezing new information out of DNA using tunable nanotopogra-phiesQueens University, Department of Physics, Ontario, Canada.

25. 26 January 2016: Squeezing new information out of DNA using tunable nanotopogra-phiesMcGill University, Department of Chemistry, Quebec, Canada.

26. 21 January 2016: Squeezing new information out of DNA using tunable nanotopogra-phiesMcGill University, Department of Physiology (CAMBAM Series), Quebec, Canada.

27. 19 June 2015: Getting into that Room at the Bottom of DNA analysis using tunablenanoscale confinementMicralyne Foundry, Alberta, Canada.

28. 13 May 2015: Nanoengineering approaches to visualizing tunable and transient molec-ular processesUniversity of California at San Diego (UCSD), Department of Bio Nano Engineering,USA.

29. 4 November 2014: Getting into that Room at the Bottom of DNA analysis usingtunable nanoscale confinementWashington State University, WA, USA.

30. 31 October 2014: Getting into that Room at the Bottom of DNA analysis usingtunable nanoscale confinementSimon Fraser University, British Columbia, Canada.

31. 30 October 2014: Getting into that Room at the Bottom of DNA analysis usingtunable nanoscale confinementUniversity of British Columbia, British Columbia, Canada.

32. 29 October 2014: Getting into that Room at the Bottom of DNA analysis usingtunable nanoscale confinementUniversity of Victoria, British Columbia, Canada.

10

33. 24 November 2014: Manipulating single-molecule dynamics with tunable nanoscaleconfinementWashington University in St Louis, Department of Physics, Missouri, USA.

34. 5 February 2014: Visualizing and controlling DNA using tunable nanoscale confine-mentMcMaster University, Department of Physics, Ontario, Canada.

35. 23 January 2014: Visualizing and controlling DNA using tunable nanoscale confine-mentQueen’s University, Department of Physics, Ontario, Canada.

36. 7 November 2013: Visualizing and controlling DNA using tunable nanoscale confine-mentUniversity of Ottawa, Department of Physics, Ontario, Canada.

37. 27 March 2013: Nano-scale confinement enables precision spectroscopy of DNA underphysiologically compelling conditionsConcordia University, Quebec, Canada.

38. 19 November 2012: Nano-scale confinement enables new kinds of biophysical mea-surementsUniversity of Montreal, Department of Physics, Quebec, Canada.

39. 5 April 2012: Nano-scale confinement enables new kinds of biophysical measurementsDalhousie University, Department of Physics, Nova Scotia, Canada.

40. 29 October 2012: Nano-scale confinement enables new kinds of biophysical measure-mentsSimon Fraser University, Department of Physics, British Columbia, Canada.

3.3 Contributed presentations at conferences

1. 13-16 Oct 2019: Deconstructing ASO-RNA hybridization with single-molecule reso-lutionOligonucleotide Therapeutics Society. Munich, Germany. Contributed talk.

2. 30 Sept - 3 Oct 2018: Single-molecule imaging empowers drug development: decon-structing structure-function relationships in oligonucleotide therapeutics.Oligonucleotide Therapeutics Society. Seattle, Washington, USA. Contributed poster.

3. 13 March 2017: Formatting biopolymers using adjustable nanoconfinementAPS March Meeting, New Orleans. March 2017. Contributed talk.

4. 13 March 2017: Manipulating and Visualizing Molecular Interactions in CustomizedNanoscale SpacesAPS March Meeting, New Orleans. March 2017. Contributed talk.

5. 12 May 2016: Squeezing new information out of DNA using tunable nanotopographiesBiology of Genomes Meeting, Cold Spring Harbor, New York, USA. Contributed poster.

11

6. 4 July 2016: Squeezing new information out of DNA using tunable nanotopographiesSingle-molecule approaches to Biology, Gordon Research Conference, Hong Kong, China.Contributed poster.

7. 29 July 2014: Manipulating and imaging DNA using tunable nanoscale confinementOSA Symposium on Optical Sensors: Micro and Nano-Engineered Sensors. Barcelona,Spain. Selected contributed talk.

8. 15 July 2014: Convex Lens-induced Nanochannel TemplatingGordon Research Conference on Single-Molecule Approaches to Biology, Italy. Con-tributed poster.

9. 10 January 2013: Nano-scale confinement enables precision spectroscopy of DNAunder physiologically compelling conditions.Aspen Center for Physics, Single-Molecule Biophysics Meeting. Organizer Tom Perkins.Selected contributed talk.

10. 23 April 2012: Shedding new light Into weak and slow molecular InteractionsBellairs Research Institute, Quantitative Biology Initiative of McGill University, Bar-bados. Contributed talk.

4 Intellectual property

4.1 Patents

1. Convex Lens-Induced Confinement (CLiC) for measuring distribution ofmolecular size: US Appl 15/234,964. Filed in August 2016 as a continuation from2010 application. Issued August 2018.A.E. Cohen, S.R. Leslie.

2. Nanofluidic platform: PCT/IB2017/000555. Filed April 2017.D.J. Berard, G. Henkin, S. R. Leslie.

3. Nanofluidic flow cell and method of loading sample: CA 2974368 and US15/654339, Filed July 2017.D.J. Berard, G. Henkin, A. Kamanzi, S.R. Leslie.

4. Nanofluidic system for molecular imaging: US 62/572673, Filed 2017.D.J. Berard, S.R. Leslie.

5. Flow cell: US 29611,297 and CA 176351, Filed 2017, Awarded 2019.D.J. Berard, A. Kamanzi, S. R. Leslie.

5 Distinctions and Awards

1. Biophysical Society of Canada Young Investigator Award, 10/2019, given to recognizeyoung Canadian scientists who have made exceptional contributions to biophysics. In-cludes conference presentation and honorarium (1k CAD).

12

2. Women in Entrepreneurship Award, 09/2019, given to startup companies founded bywomen leaders, includes seed funds for ScopeSys (100k CAD).

3. adMare Executive Institute, 09/2019, selected for cohort of 19 biotechnology leads inCanada, incl. 10 months leadership coaching, sponsored by Pfizer (value 18k CAD).

4. Keynote Speaker, 05/2019, 18th Chemical Biophysics Symposium - University of Toronto.

5. NSERC Accelerator Award, 04/2017-03/2020. Recognizes “international” career.

6. Clark Science Executive Leadership Fellowship (SELF), Fall 2016, for an “executive miniMBA”, to support scientists with relevant training in management (value 5k CAD).

7. McGill Dobson Cup Innovation Competition, 05/2015, Fourth prize (5k CAD).

8. Integrative Biology Poster Prize (Top Prize), Gordon Research Conference on SingleMolecule Approaches to Biology, Il Ciocco, Italy, 7/2010.

9. Mary Fieser Postdoctoral Fellowship, Department of Chemistry and Chemical Biology,Harvard University, 3/2009—3/2010.

10. Natural Sciences and Engineering Research Council of Canada (NSERC) Postgradu-ate Scholarship D, International, 8/2004—8/2006, and Postgraduate Scholarship A,International, 8/2002—8/2004.

11. Department of Physics Fellowship, UC Berkeley, 8/2002—8/2004.

12. Canadian Scholarship Trust Foundation Graduate Award. Awarded yearly to 5 gradu-ating undergraduates in Canada, 5/02.

13. C.K. Choi Scholarship. 1 of 5 UBC Presidential Awards to graduating undergraduates),and designation as Wesbrook Scholar, 11/2001.

14. Dorothy Gladys Studer Memorial Scholarship. Top marks in Physics, 8/2001.

15. International Undergraduate Summer School in Particle Physics and Astronomy, for 1undergraduate in Canada selected to attend, awarded full stipend. Cavendish Astro-physics Group, Cambridge, England, 7/2001.

16. WH MacInnes Scholarship in Physics and Mathematics. Top marks in Physics, 3/2001.

17. National Research Council Women in Engineering and Science Program Award, for2 summer internships, held at NRC’s Steacie Institute for Molecular Sciences in theUltrafast Science Group with Dr. David Rayner and Paul Corkum, 1/2000–5/2002.

18. Natural Sciences and Engineering Research Council of Canada (NSERC) Undergrad-uate Research Award, for research in Mary Anne White’s Materials Science Group atDalhousie University, Halifax, Canada, 5/1999–8/1999.

19. Science Scholar Designation and Dean’s Honour List, 5/1999–5/2002.

20. Undergraduate Scholars Program, Entrance scholarship to UBC, 9/1998–5/2002.

21. Governor General’s Award, National award to top student per high school, 6/1998.

13

22. BC Science Council Award,Provincial award to top science student per high school,6/1998.

6 Research funding record summary

LeslieLab has received total research funding of over $4 M to date from government and industry sources (see Table of Grants). In Canada, agency contributions are already cor-rected for overhead, which is also the case for student stipends. Approximately one third of research funds have been directed to equipment and infrastructure while the growing major-ity has been directed to operational expenses (stipends, salaries, consumables, materials and repair). Fellowships (which leverage stipends) are not included.

In Fall 2019, grant applications in prep or submitted (as PI or co-PI) include 3 CHRP proposals which have advanced past the LOI (with Pieter Cullis, UBC Biochemistry; Blair Leavitt, UBC CMMT; Corey Nislow, UBC Pharm Sci); 1 team FRQNT proposal; 1 team NFRF LOI; and 1 NSERC Steacie award (McGill nomination).

7 Supervision and courses: summary

7.1 Undergraduate student supervision

I have supervised over 40 undergraduates in research projects over the past 7-8 years at McGill (Tables 2 and 3), who have been enrolled in diverse degree programs in Physics, Quan-titative Biology, Biology and Computer Science, Mathematics and Biology, Cell Anatomy and Molecular Biology, Physics and Physiology, and Computer Science.

7.2 Graduate student and postdoc supervision

My graduate student and PDF supervision is the cornerstone of my career in scientific research and technology development, as well as the education of Canada’s next generation of scientists (Tables 4 and 5). Over the past 7-8 years of setting up a team and laboratory, I have supervised 6 MSc students (4 degrees awarded, 2 in progress), 5 PhD students (2 awarded,3 in progress), and 4 PDFs (1 moved to Harvard with a FRQNT postdoctoral fellowship; 2 have faculty positions in Canada, 1 in industry) and I currently supervise 4 current research associates. The median or typical size of my group is 15 (currently 4 undergraduate students,5 graduate students, 4 research associates, 1 visiting scientist, and myself). All 4 MSc theses and 2 PhD theses have been published. In addition to my own students, I am serving on 3 PhD student committees at McGill, and 2 at UBC and the BCCA (as collaborator).

7.3 Competitive awards won by graduate students and PDFs

Graduate students and PDFs have won competitive fellowships and awards, with my support for their proposals which fit within my program; these are listed in Table 6.

7.4 Competitive awards and prizes won by undergraduate stu-

dents

The talented undergraduate researchers in my group have won a series of fellowship awardsto support their research.

14

Number Year Agency Grant Type Total @Leslie Lab

12018-2020 NSERC Idea to Innov Individual $350,000 $350,000Title: Single molecule manipulation and imaging platform

22018-2020 ScopeSys Idea to Innov Individual $140,000 $140,000Title: Single molecule manipulation and imaging platform

32017-2022 NSERC Discovery Individual $230,000 $230,000Title: Investigating the physical principles and mechanisms of macromolecularinteractions and dynamics using single-molecule nanofluidic approaches

42017-2020 NSERC Accelerator Individual $120,000 $120,000Title: Same as above

52017-2018 NSERC Idea to Innov. Individual $125,000 $125,000Title: Nanoscale toolset for formatting and visualizing single molecules

62017-2018 ZSG SRA Individual $22,035 $15,738Title: Massively parallel linearization and chemical treatment of DNA molecules inarrays using tunable nanoscale confinement, Part 3, for Academic Associate Stipend

72017-2018 NSERC CRD Individual $152,750 $148,014Title: Massively parallel linearization and chemical treatment of DNA molecules inarrays using tunable nanoscale confinement, Part 2 for Second Year

82016-2018 NSERC CRD Team (Co) $467,940 $152,350Title: Identify and quantitate biomarkers using nanopores fabricatedby controlled breakdown

92016-2017 NSERC CRD Individual $239,411 $220,995Title: Massively parallel linearization and chemical treatment of DNA molecules inarrays using tunable nanoscale confinement

102014-2015 ZSGenetics Contract Individual $22,035 $15,738Title: CLiC confinement and visualization of polymer complexes

112015-2016 NSERC Idea to Innov. Individual $12,000 $0Title: Market assessment of molecular manipulation and nanoscopy platform

122015-2018 FRQNT Team Team (Pr) $184,000 $184,000Title: Microscopie de confinement a l’echelle nanometrique pour l’observation de ladynamique et des interactions de l’ADN et des proteines

132015 NSERC RTI Team (Pr) $150,000 $100,000Title: Visualizing and directing dynamic DNA nanostructure assembly withsingle-molecule resolution

142015 NSERC Engage Individual $25,000 $25,000Title: Single-DNA manipulation above a suspended membrane using tunablenanoscale confinement

152014 NSERC Engage Individual $25,000 $25,000Title: Imaging patterned materials under strain

162013-2016 NSERC CHRP Team (Co) $428,370 $135,000Title: Nanoconfinement based single-cell cytogenetic platform for pre-/post-implantation genetic diagnosis

15

Number Year Agency Grant Type Total @Leslie Lab

172013 NSERC Engage Individual $25,000 $25,000Title: A custom inexpensive versatile microscope

182013-2017 CFI IOF Individual $61,000 $61,000Title: Single-molecule investigations of fast biomolecular search processes

192013-2017 CFI LOF Individual $980,000 $980,000Title: Single-molecule investigations of fast biomolecular search processes

202012 NSERC Engage Individual $25,000 $25,000Title: Dramatically improved single-molecule imaging of thin molecular films

212012 NSERC RTI Individual $147,686 $147,686Title: Force and fluorescence microscopy of DNA Repair

222012-2017 NSERC Discovery Individual $125,000 $125,000Title: Single-molecule investigations of fast biomolecular search processes

23 2012-2017 McGill Startup Individual $170,000 $170,000

Cumulative All All All All $4,227,227 $3,520,518

Table 1: Grants awarded while at McGill, using established acronyms for agencies and programs. For team grants, which include multiple investigators, “Pr” vs. “Co” indicates whether I am the Principal (Pr) or a Co-applicant (Co).

7.5 Undergraduate and graduate courses taught

The courses which I have taught at McGill are listed in Table 9 along with level, enrolment numbers and format.

16

Number Name Dates Degree Type(s) of project

43 Raffles Zhu 02/2019 – present Physics Research assistant, PHYS 459

42 Zach Friedenberger 04/2018 – present Physics Research assistant, PHYS 396, 459

41 Zhi Zhang 04/2018 – present Physics and Mathematics Research assistant, PHYS 396

40 Yunwen (Wendy) Li 04/2018 – present Computer Science Research assistant

39 Gracia Gu 04/2018 – present Biology Research assistant, BIOL 480

38 Sitong (Stone) Chen 04/2018 – 05/2019 Physics Research assistant

37 Kathryn Lande 03/2017 – 08/2018 Biology Research assistant, BIOL 480

36 Bhavyaa Chandarana 03/2017 – 08/2017 Biology Research assistant

35 Brendon Mcguinness 06/2016 – 06/2018 CS and PhysicsResearch assistant, COMP 400

COMP 396

34 Ranya Virk Patel 06/2016 – 04/2017 Quantitative BiologyResearch assistant

BIOL 468

33 Laura Saunders 05/2016 – 04/2017 Honours PhysicsResearch assistant

PHYS 459

32 Barbara Gravel 01/2016 – 04/2017 Biology and MathematicsResearch assistant

BIOL 468

31 Yash Patel 01/2016 – 05/2018Computer Science

Research assistantand Biology

30 Alvin Haoran Liao 01/2016 – 05/2018Honours Mathematics

Research assistant and PHYS 459and Physics

29 Christopher Cayen-Cyr 09/2015 – 05/2016 Honours Physics PHYS 459

28 Catherine Leroux 05/2015 – 05/2016 Honours PhysicsResearch assistant

PHYS 459*

27 Julian Wolf 08/2015 – 06/2016 Majors Physics PHYS 459

27 Zhi (Mack) Zhu 01/2015 – 05/2017Computer Science Research assistant

and Biology COMP 401

26 Sophie Battat 05/2015 – 08/2015 CEGEP student Research assistant

25 Illana Naveen 05/2015 – 08/2015 CEGEP student Research assistant

24 Alexandra Valdescault 05/2014 – 08/2014 Roll’s Royce Intern Research assistant

23 Philippe Aubin 05/2014 – 08/2014 Roll’s Royce Intern Research assistant

22 Patricia Angkiriwang 05/2014 – 08/2014Honours Biophysics,

Research assistantUBC

Table 2: Undergraduates supervised in research projects while at McGill, Part 1.

17

Number Name Dates Degree Type(s) of project

21 Justin Dragoman 05/2014 – 05/2015Honours Mathematics Research assistant

and Physics

20 Andrew Caleb Guthrie 01/2014 – 05/2016 Honours Computer ScienceCOMP 396

Research assistantPHYS 459

19 Alexander Hofkirchner 01/2014 – 07/2015 Quantitative BiologyResearch assistant

BIOL 459

18 Alexander Verge 01/2014 – 08/2014 Computer ScienceCOMP 396

Research assistant

17 Jill Laurin 09/2013 – 05/2016Honours Anatomy Research assistantand Cell Biology PHYS 396

16 Yang Zhou 09/2013 – 12/2013 Comp. Sci. and Biol. PHYS 396

15 Andrew Martin 05/2013 – 01/2014 Comp. Sci.Research assistant

PHYS 396

14 Francois Michaud 05/2013 – 07/2015 Honours PhysicsResearch assistant

PHYS 396, 449, 459

13 Alec Silver 05/2013 – 05/2014 Quantitative BiologyResearch assistant

BIOL 459

12 Christopher King 05/2013 – 12/2013 PhysicsResearch assistant

PHYS 449

11 Jessica Dakkak 05/2013 – 08/2013 CEGEP student Research assistant

10 Laurel Stothers 05/2013 – 08/2013 Physics PHYS 396

09 Max Krogrius01/2013 – 05/2013

Honours PhysicsPHYS 396

01/2014 – 05/2015 PHYS 479, 459

08 Amedee d’Aboville 01/2013 – 05/2013Honours Software

PHYS 396Engineering

07 Clarence Leung 09/2012 – 05/2013Computer Science

BIOL 468and Biology

06 Alex Sylvester 09/2012 – 12/2012 Honours Physics PHYS 396

05 Matthew Tarling 05/2012 – 05/2013 PhysicsPHYS 396, 479

BIOL 396

04 Marc Rousseau 01/2012 – 08/2012 Physics Research assistant

03 Bojing Jia 05/2012 – 05/2015 Quantitative BiologyBIOL 468

Research assistantExchange with Quorum Technologies

02 Chris McFaul 01/2012 – 06/2014Physics and Research assistantPhysiology PHYS 396

PHGY 461 D1/D2

01 Daniel Berard 01/2012 – 12/2013 Honours PhysicsResearch assistant

PHYS 459, 449

Table 3: Undergraduates supervised in research projects while at McGill, Part 2.PHYS 449, 479; COMP 400: one-semester honours project or majors thesis coursePHYS 459, BIOL 459, PHGY 461 D1/D2: two-semester thesis coursesBIOL 468: one-semester honours thesis coursePHYS / BIOL 396: one-semester project course

18

Number Name Dates Current Status

1 Dr. M. Jalal Ahamed† 11/2013 – 06/2015Assistant Professor,

University of Windsor

2 Dr. Jason Leith 04/2012 – 06/2016Consultant

3 Dr. Sara Mahshid† 06/2013 – 04/2015Assistant Prof.

McGill University

4 Dr. Marjan Shayegan∗ 03/2015 – 12/2017Post-doctoral fellowHarvard University

5 Dr. Radin Tahvildari 04/2017 – presentAcademic AssociateMcGill University

6 Dr. Kimberly Metera 11/2016 – presentAcademic AssociateMcGill University

7 Dr. Romain Berti 10/2018 – presentResearch AssociateMcGill University

8 Dr. Daniel Berard 01/2019 – presentResearch AssociateMcGill University

Table 4: Post-doctoral fellows and research and academic associates supervised while at McGill.†Co-supervised with Dr. Sladek (Genome Center) and Dr. Reisner (Physics)∗ In collaboration with Dr. Michnick during 01/17-12/17

Number Name Level Dates Current status

1 Adriel ArsenaultM.Sc. 09/2012 – 08/2015 Software engineerR.A. 08/2015 – 05/2016

2 Daniel BerardM.Sc. 01/2014 – 01/2015

Research Associate, McGill PhysicsPh.D. 01/2015 – 12/2018

3 Gilead Henkin M.Sc. 05/2013 – 08/2016 PhD candidate, UCL London

4 Albert KamanziTrainee 07/2014 – 09/2014

Ph.D., McGill PhysicsPh.D. 09/2014 – present

5 John RossTrainee 07/2015 – 09/2015

Professional rowing traineeM.Sc. 09/2015 – 04/2017

6 Shane ScottTrainee 10/2012 – 01/2013

Post doctoral fellow, MunichPh.D. 01/2013 – 12/2018

7 Cynthia ShaheenTrainee 05/2016 – 09/2016

Ph.D., McGill PhysicsPh.D. 09/2016 – present

8 Frank StabileTrainee 08/2015 – 09/2015

Ph.D., McGill PhysicsPh.D. 09/2015 – present

9 Zhiyue ZhuangTrainee 07/2016 – 09/2016

PhD candidate, North CarolinaM.Sc. 09/2016 – 05/2019

10 Cameron HastieTrainee 07/2019 – present

MSc student, McGill PhysicsM.Sc. 08/2019 – present

10 Yifei GuTrainee 07/2019 – present

MSc student, McGill PhysicsM.Sc. 07/2019 – present

Table 5: Graduate students supervised; Ross, Zhuang collaborative with Dr. Mark Sutton.

19

8 Other evidence of contributions

8.1 Outreach and media - examples

1. “Future-Ready: McGill’s Sabrina Leslie, McGill Research and Innovation”.Web Link (Feb 2019)

2. “I See Molecules” - Molecule Imaging and Investigation, Studying Interactions for Sci-entific and Medical Advancement.PodCast Link (Nov 2018)

3. Panels and advisory role to high school and university students as well as new fac-ulty. Regular service; recent example is speaking on a panel on Women in Physics inChalmers Sweden (June 2019).

4. Lab tours and participation in outreach activities. Regular service; recent example isgiving lab tours as part of Women in Physics conference in Montreal (July 2019).

8.2 Conference organization - examples

1. Co-organizer of my proposed three-day Symposium at the American Chemical Society(ACS) Meeting. Responsible for inviting approximately 20 speakers and co-organizingthe session with Dr. Corey Nislow, UBC Pharm Sci (San Francisco, September 2020).

2. Member of organizational committee of Biophysical Society of Canada Meeting. Re-sponsible for two sessions including speakers from Canada, the US, and Europe (Mon-treal, May 2017).

8.3 Technology translation and commercialization

1. Leslie Lab Single-molecule Imaging Workshop. In May 2017, the Leslie Lab trained over40 industry professionals, professors, and students from Canada, the US, and Europe touse CLiC microscopy during a 2-day workshop. Students played active roles in runningthis workshop, and gained valuable professional development experience.

2. Commercialization and ScopeSys. I founded the spin-off company ScopeSys to com-mercialize the CLiC imaging technology developed by my team. ScopeSys holds theexclusive license to the core CLiC patent from Harvard, which issued in 2018, as wellas the portfolio of 4 patents from McGill, one of which issued in 2019, and three ofwhich have converted to PCT applications. ScopeSys has established a number ofearly access partnerships which has allowed it to grow organically through revenue andgrants. Currently, ScopeSys supports 4 PhD scientists funded by our NSERC i2i P2Bgrant, and 2 Mitacs interns. Its Advisory Board, including two CEOs of Canadianbiotechnology companies (James Taylor, Precision Nanosystems, and Andre Marziali,Boreal Genomics), as well as project management support, provide valuable resourcesin professional development and communication to the overall team.

20

8.4 International collaborations - current

Our work with researchers from around the world expands the multidisciplinary expertise of our biophysics & biotechnology team. It enables us to bring single-molecule resolution to crack open longstanding problems, and enhance the training and growth of trainees.

1. David Levens (NIH) and Craig Benham (UC Davis) bring biochemistry, cancer sci-ence, and theory expertise to our research on structure-mediated interactions in DNAmolecules. They are co-authors on two of our publications [6,3] and long-term, dedi-cated collaborators, with training exchanges of students between our labs (2013-present).

2. Zev Bryant (Stanford) brings expertise in Z-DNA structures and complementary mag-netic tweezer measurements of these structures using tethered DNA. Following spendingpart of my sabbatical at Stanford and an exchange visit to Montreal, we are insert-ing Z-DNA sensing probes developed by Zev’s team into our DNA plasmids to morespecifically probe Z-DNA structure formation together (2018-present).

3. Brian Munsky (Colorado State) brings complementary expertise in statistical physics,stochastic processes, and modelling to help enable rigorous analysis of new CLiC moviesof diffusing and interacting molecules. Building on interactions at two summer TellurideWorkshops, we are developing applying new analysis methods to extract distributionsof reaction rates, diffusion coefficients, and detailed mechanisms from our CLiC imagingdata together (2017-present).

4. Philip Tinnefeld (Munich) has hosted Dr. Shane Scott, my first PhD student, undera DAAD exchange fellowship between our laboratories. Together, we are applyingCLiC to advance super-resolution imaging methods such as DNA PAINT and enablenew studies of DNA nanomaterials such as origami and nanotubes, with potentialapplications to biomarker detection (2018-present).

5. Jim McGrath (Rochester) brings expertise in nanopore fabrication and microfluidics.In collaboration with Vincent Tabard-Cossa (Ottawa), we have a long-term projectto integrate nanopore devices and nanoporous membranes within our two-layer CLiCflow cell, which we have recently innovated in a miniature (5-mm) format and haveacquired preliminary data for grant applications. This ambitious collaboration beganwith a two-year NSERC CRD grant in partnership with Abbott and is focused onlong-term applications of CLiC technology to advancing diagnostics.

21

Number Recipient Fellowship Proposal Fellowship Date

1 Cameron HastieSingle-molecule microscopy NSERC CGSM MSc 09/2019of nucleic acid interactions graduate fellowship –

dependent on structure 08/2021

2 Cynthia ShaheenToward single-molecule microscopy NSERC CGSM PhD 09/2018of genome regulation mechanisms graduate fellowship –

using in-vitro DNA constructs 08/2021

1 Zhiyue ZhuangUsing adjustable nanoconfinement NSERC Bionanomachines 09/2016

to unravel and guide DNA polymers graduate fellowship –into and through nanopore sensors 03/2017

2 Cynthia ShaheenToward single-molecule microscopy NSERC Bionanomachines 09/2016of genome regulation mechanisms graduate fellowship –

using in-vitro DNA constructs 03/2017

3 Albert KamanziPartitioning DNA polymers NSERC Bionanomachines 09/2016

under applied nanoconfinement graduate fellowship –using an adjustable nanofluidic device 03/2017

4 John Ross1Wide-field correlation spectroscopy NSERC Bionanomachines 09/2015

of weak and slow interactions graduate fellowship –using CLiC kICS methodology 03/2017

5 Frank StabileNext-generation sequencing using NSERC Bionanomachines 09/2015

tunable nanoscale confinement graduate fellowship - 03/2017

6Marjan Shayegan

Toward CLiC imaging of NSERC CDMC 03/2015regulatory protein-DNA interactions PDF fellowship - 03/2016

7 Daniel BerardToward mapping genomes with CLiC NSERC CGS D fellowship 09/2015

microscopy and nanolithography - 08/2018

8 Daniel BerardToward mapping genomes with CLiC NSERC Bionanomachines 09/2014

microscopy and nanolithography MSc fellowship - 08/2015

9 Gilead HenkinToward single-molecule NSERC Bionanomachines 05/2014biomarker diagnostics MSc fellowship - 05/2016

10 Jason LeithPartitioning DNA polymers and NSERC Bionanomachines 03/2014

nanostructures using CLiC microscopy PDF fellowship - 03/2015

11 Adriel Arsenault2Toward single-molecule microscopy NSERC CDMC 09/2012

of DNA repair graduate fellowship - 08/2015

12 Shane ScottVisualizing superhelicity-driven FRQNT graduate 09/2015

transcriptional dynamics fellowship - 01/2016

13 Shane ScottVisualizing superhelicity-driven NSERC CDMC 01/2013

transcriptional dynamics graduate fellowship - 09/2015

14 Jason LeithVisualizing DNA dynamics NSERC CDMC 03/2012

using CLiC microscopy PDF fellowship - 03/2014

Table 6: Graduate students/postdocs having received competitive awards.CDMC: Cellular Dynamics of Macromolecular Complexes1Co-supervised by Paul Wiseman and Mark Sutton2Listed fellowship and research time reduced by medical leave, 10/2013 – 04/2014

22

Number Recipient Fellowship Proposal Fellowship Date

1 Raffles ZhuNanoparticle analysis and simulation

SURA05/2019

under confinement - 08/2019

2 Zachary FriedenbergerNanoparticle analysis and simulation

SURA05/2019

under confinement - 08/2019

3 Yuenwen (Wendy) LiNanoparticle fusion analysis

USRA05/2019

under confinement - 08/2019

4 Zhi ZhangAnalysis of DNA binding

USRA05/2019

under confinement - 08/2019

5 Sitong (Stone) ChenComputer vision analysis

SURA05/2018

of DNA interactions in CLiC imaging - 08/2018

6 Yuenwen (Wendy) LiNanoparticle interactions

SURA05/2018

in confinement - 08/2018

7 Alvin Haoran LiaoNanoparticle interactions

SURA05/2017

under confinement - 08/2017

8 Yash PatelNanoparticle interactions

USRA05/2017

under confinement - 08/2017

9 Alvin Haoran LiaoDNA “PAINT and CLiC”

SURA05/2016

- 08/2016

10 Yash PatelDNA “PAINT and CLiC”

Bionano Fellowship05/2016

- 08/2016

11 Laura SaundersSeeing single DNA do the twist

NSERC USRA05/2016

- 08/2016

12 Catherine LerouxCLiC visualization of molecular

NSERC USRA05/2015

reactions and complexes - 08/2015

13 Boijing JiaSqueezing cellular dynamics Industrial Undergraduate 05/2014

into focus NSERC Award - 08/2014

14 Alexander Hofkirchner Topology and TranscriptionNSERC CREATE 05/2014Bionanomachines - 08/2014

15 Patricia AngkiriwangPartitioning DNA nanostructures

NSERC USRA05/2014

using CLiC - 08/2014

16 Justin DragomanManipulating DNA with tunable NSERC USRA+CREATE 05/2014

nanoscale confinement Bionanomachines - 08/2014

17 Alexander VergeCLiC Wide-Field Fluorescence

SURA05/2014

Cross Correlation Spectroscopy - 08/2014

18 Daniel BerardConvex Lens-Induced

SURA05/2013

Nanoscale Templating - 08/2013

19 Chris McFaulConfinement spectroscopy of NSERC CREATE 05/2013

DNA molecules Bionanomachines - 08/2013

20 Alec Silver

Creating a model molecularsystem for visualizing superhlicity- NSERC CREATE 05/2013

driven conformation transitions Bionanomachines - 08/2013of DNA plasmids

21 Chris McFaulFluorescence microscopy of thin NSERC CREATE 05/2012

molecular films using CDMC - 08/2012flow-cell CLiC microscopy

22 Daniel BerardPrecision platform for Convex NSERC CREATE 05/2012

Lens-Induced Confinement (CLiC) Bionanomachines - 08/2012

Table 7: Undergraduate research fellowshipsCREATE: Collaborative Research and Training Experience (an NSERC Program)

USRA: Undergraduate Student Research Award (an NSERC Program)

SURA: Faculty of Science Undergraduate Research Award (internal McGill Program)

23

Number Name Prize Event Date

1 Haoran Liao Second prize (poster)Physical Science Division

10/2017McGill Faculty of SciencePoster Competition

2 Francois Michaud First prize (oral presentation)Biological/Medicinal Division

06/2015of Canadian ChemistrySociety Conference

3 Bojing JiaReginald Fessenden Prize Competition

12/2014in Innovation (Written Submission)

4 Alexander Verge First prize (poster and talk)Bionanomachines

08/2014Summer Symposium

5 Alexander Hofkirchner Second prize (poster and talk)Bionanomachines

08/2014Summer Symposium

6 Jill Laurin Top prize (poster and talk)Molecular Biology

08/2014Summer Symposium

7 Justin Dragoman Fourth prize (poster)Physics Department

08/2014Poster Fair

8Bojing Jia

Selected and funded to National Research01/25/2014Collegiate Conference,

attend and present a poster Harvard University

9 Bojing Jia Top poster prizeFaculty of Science Poster Fair,

10/2013Cellular and OrganismalBiological Sciences Division

10 Daniel Berard Top poster prizeFaculty of Science Poster Fair,

10/2013Physical Sciences Division

11 Daniel Berard Top poster prizePhysics Department

09/2013Poster Fair

12 Daniel Berard Top oral presentation prizeCUPC and CAP,

10/2012Biological and MedicalPhysics Division

13Daniel Berard

Top poster prizePhysics Department

09/2012& Chris McFaul Poster Fair

Table 8: Undergraduate research prizes

24

Semester Course Level Enrolment Format Duration

W20 Phys 489 Undergrad 35 Project Full SemesterF19 Phys 449 Undergrad 8 Project Full SemesterS19 Phys 479 Undergrad 7 Project Full SemesterW19 PHYS 329 Undergrad 8 Classroom Full SemesterF17 PHYS 449 Undergrad 5 Project Full SemesterS17 Phys 479 Undergrad 8 Classroom Full SemesterW17 PHYS 333 Undergrad 51 Classroom Full SemesterW17 Phys 489 Undergrad 35 Classroom Full SemesterW17 Phys 634 Grad 8 Classroom Full Semester (Coteaching)W16 BIOL PHYS 319 Undergrad 15 Classroom Full SemesterW16 BIOL PHYS 333 Undergrad 41 Classroom Full SemesterW16 PHYS 607 Grad 6 Reading/Presentation Full SemesterW16 PHYS 659 Grad 14 Classroom 1 Week + 1 ProjectW15 BIOL PHYS 319 Undergrad 22 Classroom Full SemesterW15 BIOL PHYS 333 Undergrad 38 Classroom Full SemesterW15 PHYS 659 Grad 16 Classroom 1 WeekF15 PHYS 634 Grad 5 Classroom 1/4 Semester + CoordinatorW14 BIOL PHYS 319 Undergrad 13 Classroom Full SemesterW14 BIOL PHYS 333 Undergrad 49 Classroom Full SemesterW14 PHYS 659 Grad 16 Classroom 1 WeekW13 BIOL PHYS 319 Undergrad 24 Classroom Full SemesterF13 PHYS 634 Grad 8 Classroom 1/4 SemesterF12 PHYS 607 Grad 1 Reading Full SemesterW12 BIOL 413 Undergrad 1 Reading Full SemesterW12 BIOL PHYS 319 Undergrad 17 Classroom Full Semester

Table 9: Courses instructed at McGill. Phys 329 and 33 are Statistical Mechanics. Phys Biol319 is Introduction to Biological Physics. Phys 449, 489, and 479 are one-semester Thesis ProjectCourses. Under ‘Format’, the word ‘Classroom’ indicates that lectures were delivered in a traditionalclassroom setting, in contrast with, e.g, ‘Project’ or ‘Reading/Presentation’, which use guided andactive-learning formats and less frequent meetings. The semester code indicates the semester-year,e.g., W12 indicates Winter-2012. This does not include research projects which I have served assupervisor, which are listed in other tables above.

25