Agenda

Why the Hype

Overview

INTRODUCTION

Volumetric Equipment

Best Practices for Laboratory Equipment Selection

Uses and Operation

Troubleshooting and General Maintenance

Proper Documentation

Quality Assurance

Safety

INTRODUCTION

Agenda

10 20 30 40 50 60 70 80

30

20

10

0

- 10

- 20IMPRECISION (% CV)

Inac

cura

cy %

40

*Curtis and Rodrigues | American Laboratory (Feb. 2004)

Inac

cura

cy %

10 20 30 40 50 60 70 80

40

30

20

10

0

- 10

- 20IMPRECISION (% CV)

EX

PE

RIE

NC

ED

LA

B T

EC

HN

ICIA

N

GR

AD

UA

TE

ST

UD

EN

TS

Why the Hype

INTRODUCTION

Erlenmeyer and beakers should not be used to measure only to estimate.

The amount of space

a substance occupies.

Volumetric Flasks

Glass Pipettes

Graduated Cylinders

Dispenser Pipettes

Bottle Dispensers

Burettes

Overview

INTRODUCTION

Classification

Markings

Reading the Meniscus

Flasks

Graduated Cylinders

Pipettes

VOLUMETRIC GLASSWARE

Classification Type (Class A, AS, and B) Determination

suitability for official certification degree of accuracy and error

Graduation Serial Number

Waiting & Delivery Times (pipettes)

Guided by application and equipment design

Classification (A vs. B)

VOLUMETRIC GLASSWARE

Classification

BRAND trademark for the highest quality gradevolumetric instruments

Manufacturer

Nominal volume

Tolerance(error limit)

DIN Certification mark

Reference temperature (20o C)calibration (TD, Ex = to deliver),

waiting time(15 sec.)

Class 'A', the highestquality grade,

'S' for swift delivery

Country of origin

The symbol for the conformity certification by a certifying organization

VOLUMETRIC GLASSWARE

Markings

Remember!- eye level reading- contrasting background

CONVEXupward (cohesion) high point reading mercuric

CONCAVE downward (adhesion) low point reading aqueous

VOLUMETRIC GLASSWARE

Reading the Meniscus

Types

Glass

Plastic

Class A & B

Use

Single volume (10-1000 mL)

Q.S. ('quantity sufficient') means 'bring to volume'

High accuracy volume

TC at 20 degrees Celsius

VOLUMETRIC GLASSWARE

Flasks

Capacity (mL)

Graduation Interval (primary mark; mL)

Sub-Interval (secondary mark; mL) Tolerance ( mL)

10 1 to 10 0.1 0.01

25 2 to 25 0.2 0.17

50 3 to 50 1 0.25

100 5 to 100 1 0.4

250 10 to 250 2 0.8

500 25 to 500 5 1.3

1000 50 to 1000 10 2.5

*Source: KIMAX® Class A, Serialized and Certified Kimax

VOLUMETRIC GLASSWARE

Graduated Cylinders

VOLUMETRIC GLASSWARE

Check temperature

Fill with liquid

Read meniscus

Wipe off

Deliver to wall of the vessel

'Rolling' wipe of pipette tip on wall

Strict observance of waiting time

Best Practices - Steps of use

Pipettes

Bulb

Graduated

Mohr

Serological/Tissue

Capillary (to contain up to 200 µL)

Blood

Sahli

Blood dilution

VOLUMETRIC GLASSWARE

Pipettes

Dispenser Pipettes

Bottle Dispensers

VOLUMETRIC EQUIPMENT

Techniques

Forward Pipetting

Reverse Pipetting

Diluting

Multi-dispensing or Sequenced dispensing

VOLUMETRIC EQUIPMENT

Dispenser Pipettes

Allow sample to come to ambient temperature

Pipette immersion below sample meniscus

Maintain controlled plunger pressure and speed

Best Practices - Steps of use

Dispenser Pipettes

Minimize pipette and tip handling.

VOLUMETRIC EQUIPMENT

Do not rotate the volume adjuster beyond the upper/lower range capacity

Do not allow contamination of the shaftassembly of pipette by:

operating without a tip

laying a filled pipettor on its side

immersing the barrel

allowing the plunger to snap up whenliquid is being aspirated

Best Practices - Troubleshooting

Dispenser Pipettes

Remember!Do not heat pipettes or pass through a flame.

Handle with care to prevent falls.

VOLUMETRIC EQUIPMENT

Store pipettors set to their highest volume to releases pressure on the spring mechanism

Routine maintenance and calibration checks

Competency training

Maintain the environment

Assign pipette unique and traceableidentification numbers

Evaluate ergonomics and design

Best Practices - Troubleshooting

Dispenser Pipettes

Plastic tip

Discharge end

Digital display window

Plunger

To discard tip

VOLUMETRIC EQUIPMENT

Serial work

Rapid and consistent

Replaces graduated cylinder pours

Capability

0.1 to 100 ml

Dispenser burettes

Best Practices - Use

Bottle Dispensers

Waiting time not required.

Meniscus setting not required.

VOLUMETRIC EQUIPMENT

Bottle Dispensers

Repeated discharge of identical quantities of liquid, usually reagents, at a measured amount for delivery

Major components

bottle-top lid or direct dispenser bottle

measuring cylinder/piston(e.g., floating piston, spring-loaded “wiping-seal” piston)

valve system

protection sleeve for glass cylinder

discharge tube

VOLUMETRIC EQUIPMENT

ACCURACYLOW HIGH

PREC

ISIO

NLO

WHI

GH

Accuracy and Precision

VOLUMETRIC EQUIPMENT

Additional Links

Course Documentation

SUMMARY

Upon completion

of this course you

will receive documentation

of attendance by

e-mail.

Log on to our website For more courses!

ADDITIONAL LINKS

Please complete a course survey and provide us with feedback on the course

ACCENT credit is available for this course. When you have completed this course the curriculum page will allow you access to the AACC link

Coming Soon! Uncertainty Measurements