RTB-NARO collaborations

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Presentation at RTB Annual Review and Planning Meeting (Entebbe, Uganda, 29 Sep-3 Oct 2014)

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<ul><li> 1. RTB-NARO CollaborationsUganda| 3 October 2014 Yona Baguma, PhD Acting Deputy Director General Research Coordination, NAROPlot 11-13,Lugard Avenue, P.O. Box 295, Entebbe, Ugandat: +256 (414) 320512, 320341/2 m: +256 (772) 930185, f: +256 (414) 321070Email: researchcoordination@naro.go.ug baguma1234@yahoo.com,</li></ul> <p> 2. WHAT IS NARO? Apex body responsible for guidance and coordination ofall agricultural research activities in the NARS in Uganda Nonprofit, national Old NARO established in 1992 (NAR Act 1992) New NARO established in 2005 (NAR Act 2005) About 834 staff NARO 16 PARI Centres (7 NARIs &amp; 9 Zonal) Research activities conducted by semi- autonomousPARIs coordinated by a NARO Council Secretariat 3. Biotechnology Applications toCombat Cassava Brown StreakDisease Aim: To develop and deploy new technologies to improve theaccuracy and efficiency of cassava breeding to address the cassavabrown streak disease (CBSD) epidemic Duration: 1st Phase: 2009 to 2013; 2nd Phase 2014-2016 Key Achievements 36 new breeding materials that combine CBSD and CMD tolerancedeveloped Categorization of CBSD resistance/Tolerance Human capacity built for plant breeding: 2 MSc, 1 PhD. 4. Cassava Varieties and Clean Seedto Combat CBSD and CMD (5CP) Aim: To exchange and evaluate 25 elite cassava genotypesamong NARS in CBSD affected countries, and to monitordegeneration due to CBSD in elite cassava varietiesDuration: 2012 2016Key Achievements Uganda received the 24 bestCBSD tolerant varieties from theregion Experiment to study degenerationdue to CBSD planted NARS Breeding Programmes:Kenya, Tanzania, Malawi &amp; Mozambique 5. Double Haploid Breeding forCassava Enhancement, Phase IIAim: Development of robust protocols for generation of cassavadouble haploidsDuration: 2014 - 2017Key Achievements Androgenesis was undertaken primarily on two genotypes; allregenerated plantlets not haploid Use of irradiated pollen tested at selected dosages; allregenerated plants not haploid 6. Sweetpotato Action for Health andSecurity in Africa (SASHA)Aim: Building of capacity in biotechnology and biosafety, anddevelopment of WRSP Varieties and Breeding LinesDuration: 2009-2014Key Achievements Protocol optimised for regeneration of adventitious shoots based onthidiazuron (TDZ) Optimized genetic transformation protocol to transform CvKyebandula Attempted to pyramid natural host resistance and geneticallyengineered resistance by transgenic breeding; F1 plants confirmedparent to progeny transfer of transgene 2 PhD students completed their studies 7. Field Evaluation of Transgenic S/potatoExpressingsiRNAse Protein For Resistance to SPVDAchievements Approval/Decision to conduct CFT granted by NBC 1st season evaluation completed, 2nd season planting done 8. Keeping Disease-free SweetpotatoPlanting Material Closer To FarmersEstablished 60 Net Tunnels on 30 farms 9. Development and Dissemination ofBiofortified CropsObjective Increase and/or introduce OFSP varieties in the farmingsystems of Northern and Western Uganda for combatingVAD.9 10. 2 OFSP varieties officially releasedand are being disseminated by H+SPK004 / 2006 / 1136NASPOT 12 O (24.7t/ha)NASPOT 7 /2006 /292NASPOT 13 O(38.4 t/ha) 11. Combating Banana Bacterial Wilt inFarming Communities Aim: Reduce the impact of BBW on farming communities in Eastand Central Africa using cultural methods Key Achievements Fine tuned control package to incorporate single stem removal; Less laborious, No need for replanting; quick production recovery Used in TZ, DRC, Rwanda, Burundi and Kenya Improved technology promotion; a combination of participatory and topdown approaches andeffectively scaled out BBW control from village to regional level Reduced BBW prevalence from 42% to below 13% in 1 year inAnkole; with yield recovery worth US$ 54.4 million. 12. Development of banana bacterialwilt (BBW) transgenic resistance Aim: To develop and deploy bananas resistant to BBW to addressthe epidemic that is very destructive and rapidly spreading with nosource of resistance for classical banana improvement. Duration: 2nd Phase 2012-2016 Key Achievements 6 lines immune to BBW already being further evaluated in CFT atNARL; to be evaluated in 2 other locations in Bulindi and Mbarara. Over 100 lines of 2 popular cultivars of M9 (with backgroundresistance to black sigatoka and Fusarium wilt) and Nakitembedeveloped with stacked genes of hrap and pflp batch has materialfor commercial release in 2020 Human capacity built in molecular breeding; 1 students being trainedat PhD. 13. Banana Weevil and NematodeTransgenic Resistance Aim: To develop and deploy transgenic banana resistant for bananaweevil and nematode resistance Duration: 2011-2015 Key Achievements Over 100 lines of Gonja and Nakitembe with resistance to bothweevils and nematodes developed and ready for screenhouseevaluation. 6 Embryogenic cell lines for Nakitmbe and 3 lines of Gonjadeveloped 14. Development of Matooke ResistantTo Black Sigatoka Aim: To develop and deploy matooke resistant for black sigatoka Duration: 2011-2015 Key Achievements 2 hybrids in AYT on farmers fields before release 3 promising hybrids in PYT at NARL and on farmers fields 2 segregating populations for Fusarium wilt and weevilsdeveloped Over 400 accessions in Musa germplasm serving the ECAregion Diploids developed for further matooke improvement 15. Field evaluation of genetically modified potatoes forresistance to potato blight disease caused byPhytophthora infestans at Kachwekano ZARDIAim: To evaluate two potato cultivars (Desiree and Victoria) overexpressing the R genes; RB, Rpi-blb2 and Rpi-vnt1.1 in the field atKachwekano, Kabale district in Uganda.Duration: 2014-2016Key AchievementsGreenhouse and field sitecontraction completedStaff trained in Biosafety of CFTsCFT application submitted toNBCMock trial with non-GM Desireeand Victoria cultivars completed 16. Considerations of IncreasedVisibility of RTB in NARO Work jointly with RTB to identify, pilot, launch and commercializeresearch products to the market, including strong aspects of valueaddition. Proposal to have NARO-Directors and Team Leaders attend RTBbusiness and/or enterprise planning meetings. Placement of RTB staff at both NARO research institutes andheadquarters to jointly plan and implement pre-selected activitiestogether. Use of new genomics approaches (genotyping by sequencing,comparative genomics, QTL mapping and gene discovery) todevelop new varieties with yield and durable resistance value. Joint effort in establishing functional seed systems for Cassava, </p>