romeo - abim · romeo treatment mimics a microbial attack perception by the plant activation of...

© Lesaffre 2015

Corporate Communications

All rights reserved

ROMEO®

Innovative biofungicide against foliar fungal diseases

ABIM, Oct.24-26, 2016 Bernard Blum Award

© Lesaffre 2015


All rights reserved

Agrauxine – Lesaffre Plant Care

• Lesaffre is an industrial family company

• 160 years experience in fermentation

• Innovation in microbials and derivates

• Scalability of the production

• Application : Biocontrol & Biostimulant

• Obtained by fermentation processes

• R&D > Registration > Production > Sales

© Lesaffre 2015


All rights reserved

Cerevisane® – Active substance

• Cell wall of S.cerevisiae yeast strain LAS117

• Specific process of purification

• Glucans, mannans, proteins, lipids and chitins

• EU approval of cerevisane on the 23/04/2015

for 15 years - Listed in annex of regulation

540/2011 as a low risk substance

• No MRLs (Annex IV of Regulation EC 396/2005)

• Cerevisane is coming from Lesaffre R&D pipeline and patented in 2006

Cerevisane microscopic picture (SEM)

© Lesaffre 2015


All rights reserved

ROMEO® – Product description

Crops Targets Dose /ha

Grapevine & Table grape Downy mildew Powdery mildew Botrytis cinerea

0,25 kg

Vegetables Cucurbits, salads, tomato, strawberry

0,5 - 0,75 kg

• Composition: 94,1% of a.i. Cerevisane

• Formulation : WP

• 2 years shelf life at room temperature

• Developped in field since 2009

• In several countries and crops (> 300 GEP trials)

• Partnership with universities, technical institutes and distribution

• Preventive foliar application at low dose rate

• Non living, easy to use in tank mix with chemicals

© Lesaffre 2015


All rights reserved

Field results - Grapevine Downy Mildew

• Very strong pressure of downy mildew on bunches • In tank mix, ROMEO allowed 50% dose reduction of copper

5,0

34,5

69,2

79,0

0,1 0,8 4,0

6,0

0,3 3,4

8,1

19,0

0,3 2,5 3,7

6,0

0

10

20

30

40

50

60

70

80

90

02/06 09/06 27/06 12/07

% s

everit

y o

n b

un

ch

es

Assessment date

Grapevine Downy Mildew - Portugal 2016 % Severity on bunches

UTC

KOCIDE 2000

300g/hL

KOCIDE 2000

150 g/hL

ROMEO 0,25kg

+ KOCIDE 2000

150 g/hL

© Lesaffre 2015


All rights reserved

Field results - Grapevine Powdery Mildew

*REF . Program = Tebuconazole+Triadimenol / Fluopyram + Trifloxystrobin / Metrafenone / Proquinazid / Metrafenone / Spiroxamine

32,7

43,5

49,2 50,0

2,4

7,6

11,3 10,3

5,8

14,6

17,6 17,9

3,7

9,4

13,2 11,2

0

10

20

30

40

50

60

06/06 20/06 05/07 19/07

% s

everit

y o

n l

eaves

Assessment date

Grapevine Powdery Mildew – France 2016 % Severity on leaves

UTC

REF. program

REF. program 50%

ROMEO 0,25kg +

REF program 50%

• Strong pressure and early attack on Carignan cultivar • In tank mix, ROMEO allowed 50% dose reduction of this chemical

program

© Lesaffre 2015


All rights reserved

Field results - Cucurbits Powdery Mildew

• Very strong pressure of powdery mildew on leaves • In alternation with sulfur, ROMEO improved the efficacy and

reached azoxystrobin reference efficacy.

3,8

27,5

68,7

84,9

0,1 2,8

26,2

32,2

2,0

19,0

44,2 48,4

0,0

8,6

31,0 34,4

0

10

20

30

40

50

60

70

80

90

100

20/06 28/06 05/07 12/07

% s

everit

y o

n u

pp

er l

eaves

Assessment date

Melon Powdery Mildew - Spain 2016 % Severity on upper leaves

UTC

ORTIVA

THIOVIT JET

(BD)

ROMEO 0,5kg

(ACE) / THIOVIT

JET (BD)

c

bc

a

b

ab

a

b

ab

a

(ABCDE)

© Lesaffre 2015


All rights reserved

And finally how does it works ?

ROMEO treatment

mimics a microbial attack

Perception by

the plant

Activation of cell

signaling cascade

Induction of

defense genes

Metabolic changes

= effective defenses

Plant protection against

further pathogen attacks

UTC ROMEO

ROMEO stimulates the production of phytoalexins in leaves (fluorescent compounds).

Microscopic observation of E.necator development on grapevine leave

UTC ROMEO®

© Lesaffre 2015


All rights reserved

And finally how does it works ? Genomic studies

• ROMEO induces the expression of many genes including genes involved in defense

• Stimulates both SA- and JA/ET- dependent signaling pathways

• Activates some defense processes known to be implicated in the reaction of grapevine genotypes resistant to downy mildew.

0,1 0,4

12,9

1,9

0

2

4

6

8

10

12

14

16

18

20

PR1 PR4

expre

ssio

n level (R

PKM

)

Expression level (RPKM) of PR1 & PR4 1 day after ROMEO foliar application

UTC

ROMEO

© Lesaffre 2015


All rights reserved

CONCLUSION Innovative biocontrol solution

• 1st Systemic Resistance Inducer based on non living microbial

• Multi-targets: effective against several foliar fungal diseases

• Easy to integrate in chemical program and manage dose reduction

• Long shelf life (2 years) and available at industrial scale

• Low dose rate : 0,25 – 0,75 kg/ha

• EU approval of active substance Cerevisane – Low risk

• No residues

PERFORMANT BY NATURE

romeo - abim · romeo treatment mimics a microbial attack perception by the plant activation of...

Documents