Roadway pollutant effects Roadway pollutant effects on abundance of Odonate on abundance of Odonate nymphs in a wetland of nymphs in a wetland of the Eastern Highland Rim, the Eastern Highland Rim, TennesseeTennessee

Jason Payne, Undergraduate Student (Marine Jason Payne, Undergraduate Student (Marine Biology Concentration), Department of Biology, Biology Concentration), Department of Biology, Tennessee Technological University, Cookeville, Tennessee Technological University, Cookeville, TN. 38505TN. 38505

Does pollution from roadways Does pollution from roadways affect the abundance of affect the abundance of

Odonate nymphs?Odonate nymphs? ObjectiveObjective: Collect odonate nymphs at a control : Collect odonate nymphs at a control

area (3), transitional area (2) , and impact area area (3), transitional area (2) , and impact area (1) at the wetland.(1) at the wetland. Use a small boat to get to desired areasUse a small boat to get to desired areas Collect with a D-Frame netCollect with a D-Frame net Put samples in bagsPut samples in bags Expected results:Expected results:

Less Odonate nymph abundance at the impact and Less Odonate nymph abundance at the impact and transitional areas than the control.transitional areas than the control.

IntroductionIntroduction Osborn, R. 2005. Odonata as indicators of habitat Osborn, R. 2005. Odonata as indicators of habitat

quality at Lakes in Louisiana, United States. quality at Lakes in Louisiana, United States. Odonatologica 34(3):259-270Odonatologica 34(3):259-270

Crossman, J.S. and J.R. Cairns. 1974. A comparative Crossman, J.S. and J.R. Cairns. 1974. A comparative study between two different artificial substrate study between two different artificial substrate samplers and regular sampling techniques. samplers and regular sampling techniques. Hydrobiologia 44(4):517-522Hydrobiologia 44(4):517-522

Elliot, J.M. 1971. Statistical analysis of samples of Elliot, J.M. 1971. Statistical analysis of samples of benthic invertebrates. Freshwater Biological benthic invertebrates. Freshwater Biological Association Scientific Publication No. 25.Association Scientific Publication No. 25.

HypothesisHypothesisCars leak oil and other pollutants causing a Cars leak oil and other pollutants causing a

decline in abundance of odonate nymphs.decline in abundance of odonate nymphs.

Null HypothesisNull HypothesisPollutants from cars have no affect on Odonate Pollutants from cars have no affect on Odonate

nymphs.nymphs.

Methods and MaterialsMethods and Materials Scoop sediment with a D-Frame net while Scoop sediment with a D-Frame net while

disturbing the branches of aquatic plants at disturbing the branches of aquatic plants at the wetland.the wetland. Place in 3 one-gallon zip-lock sample bags from the 3 Place in 3 one-gallon zip-lock sample bags from the 3

sitessites Treat with alcoholTreat with alcohol Sort through samples finding Odonate nymphs Sort through samples finding Odonate nymphs Determine the averages of Odonate nymphs from the Determine the averages of Odonate nymphs from the

3 sample bags from each site and compare.3 sample bags from each site and compare. Data FormData Form

Expected Results and Expected Results and Benefits Benefits

I expect the abundance of nymphs at the I expect the abundance of nymphs at the impact area will be less than the controlimpact area will be less than the control

This experiment will contribute to the This experiment will contribute to the understanding of the fragile ecosystem of understanding of the fragile ecosystem of wetlands in the presence of pollutants.wetlands in the presence of pollutants.

TimelineTimeline Timescale graph on my researchTimescale graph on my research TimelineTimeline

BudgetBudget Hypothetical budget in one yearHypothetical budget in one year BudgetBudget