reversed-phase columns (other than ods) · pdf fileymc-pack series include a variety of...

YMC GENERAL CATALOG

2Reversed-Phase Columns(Other than ODS)

Types and characteristics of reversed-phase columns (other than ODS)

YMC-Pack Pro C8、C4YMC-Pack C8

YMC-Pack C4

YMC-Pack TMSYMC-Pack PhYMC-Pack CNYMC-Pack PROTEIN-RPYMCbasicYMC Carotenoid

58,59

60～6364,6566,6768,6970,7172,7374,75

7677

57

第02章.indd 57 11.1.28 10:46:30 AM

58

2

Reversed-P

hase Colum

ns (Other than O

DS

)

Types and characteristics of reversed-phase columns (other than ODS)

Product NamePore size（Å）

Particlesize

（µm）C% Silanol

treatmentUsable

pH range Characteristics Page

Pro seriesPro C8 120 3, 5 10 Yes

2.0 ～ 7.5

・ Processed with advanced endcapping technology

・ Superior separation of basic compounds 60 ～ 63

Pro C4 120 3, 5 7 Yes・ Processed with advanced

endcapping technology・Different selectivity from ODS

YMC-Pack seriesC8

120 3, 5,10 10Yes ・Moderate hydrophobicity 64,65200 5,10 7

300 5,10 4

C4

120 3, 5,10 7Yes ・ Lower hydrophobicity than

ODS and C8 66,67200 5,10 5300 5,10 3

TMS 120 3, 5,10 4 Yes ・ Reversed-phase packing material with the lowest hydrophobicity 68,69

Ph 120 3, 5,10 9 Yes ・ Reversed-phase packing material with π electrons 70,71

CN 120 3, 5,10 7 Yes ・ Can be used in both normal-phase and reversed-phase modes 72,73300 5 3

PROTEIN-RP 200 5 4 — 1.5 ～ 7.5 ・ Useful for separation of proteins and peptides 74,75

YMCbasic 200 3, 5 7 Yes 2.0 ～ 7.5 ・ Superior separation of basic compounds 76

YMC Carotenoid — 3, 5 — — 2.0 ～ 7.5 ・ Useful for carotenoids separation 77

Elution behavior dependent on alkyl chain lengthIn reversed-phase chromatography, retention due to hydrophobicity generally depends directly on the carbon number of the stationary phase. The degree of retention due to hydrophobicity of the stationary phase can generally be listed in descending order by column type as ODS＞C8＞C4＞TMS. Stationary phases with low hydrophobicity can be used effectively to reduce analysis time for samples having too strong of a retention on ODS. Stationary phases with low hydrophobicity are also useful for samples that are slightly soluble in organic solvents and need to be analyzed with mobile phase containing a low concentration of organic solvents.

Types of reversed-phase columns（Ⅰ）

ODS -C18H37 Retention due to hydrophobicity

High

Low

C8 -C8H17

C4 -C4H9

TMS -CH3

Elution behavior affected by other factorsCN and Ph have available π electrons derived from their functional groups. CN and Ph sometimes show different separation characteristics from stationary phases that are chemically bonded with straight alkyl chains. Since CN has medium-polar functional groups, it can be used in both normal-phase and reversed-phase separation modes, depending on the mobile phase used.

Types of reversed-phase columns（Ⅱ）

Ph πelectrons available

CN -(CH2)3-CNπelectrons

availableCan be used also in normal-phase

Types and characteristics of reversed-phase columnsYMC-Pack Pro C8、C4YMC-Pack C8、C4、TMS、Ph、CN

YMC-Pack series include a variety of reversed-phase columns other than ODS, enabling column selection from a wide range of products to suit the sample characteristics.

第02章.indd 58 11.1.28 10:46:32 AM

59

Reversed-P

hase Colum

ns (Other than O

DS

)

2

Elution behavior dependent on alkyl chain length（1）

1. Uracil2. Methyl benzoate3. Progesterone4. n-Butyl benzoate5. n-Butylbenzene6. n-Amylbenzene

Column ：150×4.6mmI.D.Eluent ：acetonitrile/water(60/40)Flowrate ：1.0mL/minTemperature：37℃Detection ：UVat260nm

2

13

45

6

100 20 30

YMC-Pack ODS-A

2

13

4

5

6

10 20 300

YMC-Pack C8

24

5

6

3

1

10 200

YMC-Pack C44

2

1

5

63

10 200

YMC-Pack TMS

Elution behavior dependent on alkyl chain length（2）

1. Toluene2. Deoxycorticosterone acetate3. Imipramine hydrochloride4. Triphenylene5. n-Amylbenzene

Column ：150×4.6mmI.D.Eluent ：20mMKH2PO4-K2HPO4(pH6.9)/methanol(25/75)Flowrate ：1.0mL/minTemperature：37℃Detection ：UVat254nm

0 5 10 15 20 25min

1

2

34

5

Pro C18

0 5 10 15 20min

1

2

3

4

5

Pro C8

0 5 10min

1

2

3

4

5

Pro C4Inthisexample,theretentionbehaviorofavarietyofcompoundsisshowntobedependentonthealkylchainlengthofthestationaryphase.ShorteralkylchainlengthslikeC4showreducedretentionforneutralcompoundsduetothediminishedhydrophobicityoftheC4stationaryphaserelativetolongeralkylchainsphaseslikeC8andC18.Thedifferencesintheselectivityofstationaryphasesofdifferentalkylchainlengthisalsoillustratedfortriphenylene,aplanarmoleculewithrestrictedrotationalmovement.TriphenyleneshowsmuchshorterretentiononC4relativetoC8andC18thanwouldbeexpectedonthebasisofhydrophobicityofthestationaryphase.Notethedifferenceinelutionorderfortriphenylenerelativetoimipramineandamylbenzeneforthismixtureonthisseriesofstationaryphases.

Different separation behaviors of Ph and ODS

13

2

4

10 200 min

YMC-Pack Ph

1

42

3

100 20 min

YMC-Pack ODS-AM DifferencesinretentionbehaviorofPhandODSforanalysisofwater-solublevitaminsareshownontheleft.TheelutionorderofriboflavinandcyanocobalaminonPhisthereverseofthatonODS.Thisisconsideredtobearesultofstrongretentionofcyanocobalaminduetoπ-πinteractioncausedbyphenylgroup.

1. p-Aminobenzoic acid (PABA)2. Biotin (Vitamin H)3. Riboflavin (Vitamin B2)4. Cyanocobalamin (Vitamin B12)

Column ：150×4.6mmI.D.Eluent ：acetonitrile/50mMNH4H2PO4(10/90)Flowrate ：1.0mL/minTemperature：37℃Detection ：UVat210nm,0.16AUFSInjection ：10µL(0.02～ 0.30mg/mL)

第02章.indd 59 11.1.28 10:46:36 AM

60

2

Reversed-P

hase Colum

ns (Other than O

DS

)

Analytical columns

YMC-Pack Pro C8, C4● Superior separation of basic compounds● Processed with the same advanced

endcapping technology as Pro C18

● Excellent reproducibility● Utilizes highly pure silica gel base● Two types of test reports are included

with each column

H i g h l y e n d c a p p e d C8 a n d C4 r e v e r s e d - p h a s e c o l u m n sYMC-PackProC8andC4arehighlyappropriateforbasiccompoundssincemoreadvancedendcappingtechnologyisusedforprocessingoftheirresidualsilanolgroupsthatarelikelytoaffectquality.TheYMC-PackProC8andC4stationaryphasesurfacehydrophobicityislowerthanthatofODS,

makingYMC-PackProC8andC4usefulforquickanalysisofcompoundsthatdiffergreatlyinhydrophobicity.TheseparationbehaviorofhydrophiliccompoundsorplanarcompoundsonYMC-PackProC8andC4alsodiffersfromthatonODS,makingYMC-PackProC8andC4usefulforseparatingcompoundsincaseswhereseparationoptimizationisdifficulttoachieveusingODS.

C8■Poresize：120Å ■Carboncontent：10％ ■UsablepHrange：2.0～ 7.5C4■Poresize：120Å ■Carboncontent：7％ ■UsablepHrange：2.0～ 7.5

Separation of basic compounds

EffectiveendcappingofYMC-PackProC8andC4resultsingoodpeakshapesforbasiccompoundssuchasalkaloids.

Alkaloids

1. Scoporamine bromide2. Atropine sulfate3. Cinchonine4. Quinine5. Papaverine chloride

Shorten analysis time

Examplesofseparationofanalgesic/antipyreticdrugsonProC8,C4andC18areshownabove.Sinceibuprofenhasrelativelyhighhydrophobicity,muchtimeisrequiredtoanalyzeitusingthehighlyhydrophobicpackingmaterialProC18.WhenProC8orC4isused,thelowerhydrophobicityofthepackingmaterialcanshortentheanalysistime.Inaddition,ProC8andC4achievegoodseparationofhydrophiliccompounds,includingcaffeine,ethenzamideandbromovalerylurea.

Column ：150×4.6mmI.D.Eluent ：methanol/20mMH3PO4(60/40)Flowrate ：1.0mL/minTemperature：37℃Detection ：UVat254nm

Analgesic/Antipyretic drugs

1. Caffeine2. Ethenzamide3. Bromovalerylurea4. Ibuprofen

0 5 10 15 20 25 min

1 2

3

4

5

0 10 20 30 min 0 10 20 30 min0 10 20 30 min

1

2

3

4

1

2

3

41

2

3

4

Pro C18 Pro C8 Pro C4

0 10 20 30 min

1

2

3

4

Pro C8

0 10 20 30 min

1

2

3

4

Pro C4

Column ：YMC-PackProC8(5µm,120Å) 150×4.6mmI.D.Eluent ：acetonitrile/20mMKH2PO4(20/80)Flowrate ：1.0mL/minTemperature：30℃Detection ：UVat210nm

（A000303C）

第02章.indd 60 11.1.28 10:46:38 AM

61

Reversed-P

hase Colum

ns (Other than O

DS

)

2

6 7

0 10 20 30 40 min

Buffer / MeOH (40/60)

1

2

34

5

Pro C8

0 10 20 min

Buffer / MeOH (40/60)

1

2

34

5

67

Pro C4

Optimization of separation using Pro C8 and Pro C4

RetentiontimesofanalytesonProC8andProC4tendtobeshorterthanthoseonC18.Whenalkylchainlengthsofpackingmaterialfunctionalgroupsareshorter,hydrogen-bondingcapacitiestendtobegreater;therefore,notonlyretentiontime,butalsoseparationselectivityofProC8andProC4maydifferfromthoseofC18.SeparationoptimizationisdifficulttoachieveforantiarrhythmicsusingProC18,evenifthemobilephaseischanged.Incontrast,C8andC4cancompletelyseparateantiarrhythmicsinashorttime.Asshownabove,C8andC4maybeusefulincaseswhereseparationoptimizationisdifficulttoachieveusingC18.

1. Phenytoin2. Propranolol HCl3. Quinidine4. Lidocaine5. Diltiazem HCl6. Verapamil HCl7. Nicardipine HCl

Column ：150×4.6mmI.D.Eluent ：20mMKH2PO4-K2HPO4(pH6.9)/methanolFlowrate ：1.0mL/minTemperature：37℃Detection ：UVat220nm

Separation of antiarrhythmics

0 10 20 30 min

1

2

3

4 5

6 7

Buffer / MeOH (35/65)Pro C18

0 10 20 30 40 50 min

1

2

3

45

6 7

Buffer / MeOH (40/60)Pro C18

Optimization of mobile phase

第02章.indd 61 11.1.28 10:46:41 AM

62

2

Reversed-P

hase Colum

ns (Other than O

DS

)

Analytical columns

YMC-Pack Pro C8, C4

1

2

34

5

0 5 10 15 20 min

0 5 10 15 20 25 min

1

2

3

5

4

Pro C18

Pro C8

Pro C8

Column ：

Eluent ：

Flow rate ：Temperature ：Detection ：

YMC-Pack Pro C8 and Pro C18150×4.6 mmI.D.20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (25/75)1.0 mL/min37 ℃UV at 254 nm

1.Toluene2.Deoxycorticosterone acetate3.Imipramine hydrochloride4.Triphenylene5.n-Amylbenzene

0 10 20min

0 10 20 30 40 min

12

34 5 6

21

4

3

5 6

Pro C18

Pro C4

Pro C4

Column ：

Eluent ：

Flow rate ：Temperature ：Detection ：

YMC-Pack Pro C4 and Pro C18150×4.6 mmI.D.20 mM KH2PO4-K2HPO4 (pH 6.9)/methanol (35/65)1.0 mL/min37 ℃UV at 254 nm

1.Toluene2.Testosterone3.Acenaphthene4.n-Propylbenzene5.n-Butylbenzene6.Amitriptyline hydrochloride

Comparison of separation characteristics

ThesechromatogramsshowcomparisonoftheseparationcharacteristicsofProC8,ProC4andProC18.ProC8andProC4allowanalysisofcompoundswithgreatlydifferenthydrophobicityinashortertimethanProC18.Thesecolumnsalsoshowdifferentretentioncharacteristicsforhydrophilicorplanarcompoundsduetodifferentalkylchainlengthofthestationaryphases.Forsomegroupsofcompounds,ProC8andProC4mayprovidebetterseparationbehaviorinashortertime.

第02章.indd 62 11.1.28 10:46:43 AM

63

Reversed-P

hase Colum

ns (Other than O

DS

)

2

YMC-Pack Pro C8Analytical columns

Particle size（µm）

Pore size（Å）

Column sizeinnerdiameter×length（mm） Product number

S-3 120 2.0× 35 OS12S03-H502WT2.0× 50 OS12S03-0502WT2.0× 75 OS12S03-L502WT2.0×100 OS12S03-1002WT2.0×150 OS12S03-1502WT3.0× 50 OS12S03-0503WT3.0×100 OS12S03-1003WT3.0×150 OS12S03-1503WT4.6× 35 OS12S03-H546WT4.6× 50 OS12S03-0546WT4.6× 75 OS12S03-L546WT4.6×100 OS12S03-1046WT4.6×150 OS12S03-1546WT

S-5 120 1.0×150 OS12S05-1501WT1.0×250 OS12S05-2501WT1.5×150 OS12S05-15P5WT1.5×250 OS12S05-25P5WT2.0× 50 OS12S05-0502WT2.0× 75 OS12S05-L502WT2.0×100 OS12S05-1002WT2.0×150 OS12S05-1502WT3.0× 50 OS12S05-0503WT3.0×150 OS12S05-1503WT3.0×250 OS12S05-2503WT4.6× 50 OS12S05-0546WT4.6× 75 OS12S05-L546WT4.6×100 OS12S05-1046WT4.6×150 OS12S05-1546WT4.6×250 OS12S05-2546WT6.0×150 OS12S05-1506WT

Semi-preparative columnsS-5 120 20×250 OS12S05-2520WT

Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 OS12S03-0102CC

4.0×23 OS12S03-G304CC

S-5 120 1.0×10 OS12S05-0101CC1.5×10 OS12S05-01P5CC2.0×10 OS12S05-0102CC4.0×23 OS12S05-G304CC

Aguardcartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.

Cartridge holderSemi-micro guard cartridge holder(inner diameter: 1.0, 1.5, or 2.0 mm)

XPCHSMW

Cartridge holder(inner diameter: 4.0 mm)

XPCHW

Guard columnsS-5 120 20×50 OS12S05-0520WTG

YMC-Pack Pro C4Analytical columns


Pore size（Å）


S-3 120 2.0× 35 BS12S03-H502WT2.0× 50 BS12S03-0502WT2.0× 75 BS12S03-L502WT2.0×100 BS12S03-1002WT2.0×150 BS12S03-1502WT3.0× 50 BS12S03-0503WT3.0×100 BS12S03-1003WT3.0×150 BS12S03-1503WT4.6× 35 BS12S03-H546WT4.6× 50 BS12S03-0546WT4.6× 75 BS12S03-L546WT4.6×100 BS12S03-1046WT4.6×150 BS12S03-1546WT

S-5 120 1.0×150 BS12S05-1501WT1.0×250 BS12S05-2501WT1.5×150 BS12S05-15P5WT1.5×250 BS12S05-25P5WT2.0× 50 BS12S05-0502WT2.0× 75 BS12S05-L502WT2.0×100 BS12S05-1002WT2.0×150 BS12S05-1502WT3.0× 50 BS12S05-0503WT3.0×150 BS12S05-1503WT3.0×250 BS12S05-2503WT4.6× 50 BS12S05-0546WT4.6× 75 BS12S05-L546WT4.6×100 BS12S05-1046WT4.6×150 BS12S05-1546WT4.6×250 BS12S05-2546WT6.0×150 BS12S05-1506WT

Semi-preparative columnsS-5 120 20×250 BS12S05-2520WT

Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 BS12S03-0102CC

4.0×23 BS12S03-G304CC

S-5 120 1.0×10 BS12S05-0101CC1.5×10 BS12S05-01P5CC2.0×10 BS12S05-0102CC4.0×23 BS12S05-G304CC



XPCHSMW


XPCHW

Guard columnsS-5 120 20×50 BS12S05-0520WTG

Ordering information

第02章.indd 63 11.1.28 10:46:44 AM

64

2

Reversed-P

hase Colum

ns (Other than O

DS

)

Analytical columns

YMC-Pack C8

● Stationary phase with lower hydrophobicity than ODS● Useful for separating samples with relatively high hydrophobicity

R e v e r s e d - p h a s e c o l u m n w i t h m o d e r a t e h y d r o p h o b i c i t yThehydrophobicityofYMC-PackC8ismoderateforareversed-phasepackingmaterial.RetentiontimesofsamplesonYMC-PackC8tendtobeshorterthanthose

onODSstationaryphase.ThemoderatehydrophobicityofYMC-PackC8makesitusefulforseparatingsampleswithrelativelyhighhydrophobicity.

■Poresize：120Å,200Å,300Å■Carboncontent：10％ ,7％ ,4％■UsablepHrange：2.0～ 7.5

10 20

21

3

4

0 min

Anti-HIV nucleoside derivatives

HN

O

O

N

NN

HOH C2

ddI

N

O

O

NH

N

HOH C2

2

ddC

HN

O

O

O

N

HOH C2

CH3

d4T

HN

O

O

O

N

HOH C2

CH3

3

AZT

N

1. 3. 4.2.

Column ：YMC-PackC8(5µm,120Å) 150×4.6mmI.D.Eluent ：methanol/10mMKH2PO4(10/60)Flowrate ：1.0mL/minTemperature：37℃Detection ：UVat254nm

A p p l i c a t i o n （K930311A）

Analytical columnsParticle size（µm）

Pore size（Å）


S-3 120 2.0× 35 OC12S03-H502WT2.0× 50 OC12S03-0502WT2.0× 75 OC12S03-L502WT2.0×100 OC12S03-1002WT2.0×150 OC12S03-1502WT3.0× 50 OC12S03-0503WT3.0×100 OC12S03-1003WT3.0×150 OC12S03-1503WT4.6×100 OC12S03-1046WT4.6×150 OC12S03-1546WT

S-5 120 1.0×150 OC12S05-1501WT1.0×250 OC12S05-2501WT1.5×150 OC12S05-15P5WT1.5×250 OC12S05-25P5WT2.0×150 OC12S05-1502WT2.0×250 OC12S05-2502WT4.6× 75 OC12S05-L546WT4.6×100 OC12S05-1046WT4.6×150 OC12S05-1546WT4.6×250 OC12S05-2546WT4.6×300 OC12S05-3046WT6.0×100 OC12S05-1006WT6.0×150 OC12S05-1506WT6.0×250 OC12S05-2506WT6.0×300 OC12S05-3006WT

200 4.6×150 OC20S05-1546WT4.6×250 OC20S05-2546WT

300 1.0×150 OC30S05-1501WT1.0×250 OC30S05-2501WT


Pore size（Å）


S-5 300 1.5×150 OC30S05-15P5WT1.5×250 OC30S05-25P5WT2.0×150 OC30S05-1502WT2.0×250 OC30S05-2502WT4.6× 75 OC30S05-L546WT4.6×100 OC30S05-1046WT4.6×150 OC30S05-1546WT4.6×250 OC30S05-2546WT4.6×300 OC30S05-3046WT6.0×100 OC30S05-1006WT6.0×150 OC30S05-1506WT6.0×250 OC30S05-2506WT6.0×300 OC30S05-3006WT

S-10 120 4.6×100 OC12S11-1046WT4.6×150 OC12S11-1546WT4.6×250 OC12S11-2546WT4.6×300 OC12S11-3046WT6.0×100 OC12S11-1006WT6.0×150 OC12S11-1506WT6.0×250 OC12S11-2506WT6.0×300 OC12S11-3006WT

300 4.6×100 OC30S11-1046WT4.6×150 OC30S11-1546WT4.6×250 OC30S11-2546WT4.6×300 OC30S11-3046WT6.0×100 OC30S11-1006WT6.0×150 OC30S11-1506WT6.0×250 OC30S11-2506WT6.0×300 OC30S11-3006WT


第02章.indd 64 11.1.28 10:46:46 AM

65

Reversed-P

hase Colum

ns (Other than O

DS

)

2

Semi-preparative columnsParticle size（µm）

Pore size（Å）


S-5 120 10×150 OC12S05-1510WT10×250 OC12S05-2510WT10×300 OC12S05-3010WT20×100 OC12S05-1020WT20×150 OC12S05-1520WT20×250 OC12S05-2520WT

200 20×150 OC20S05-1520WT20×250 OC20S05-2520WT

300 10×150 OC30S05-1510WT10×250 OC30S05-2510WT10×300 OC30S05-3010WT20×150 OC30S05-1520WT20×250 OC30S05-2520WT

S-10 120 10×150 OC12S11-1510WT10×250 OC12S11-2510WT10×300 OC12S11-3010WT20×150 OC12S11-1520WT20×250 OC12S11-2520WT

200 20×150 OC20S11-1520WT20×250 OC20S11-2520WT

300 10×150 OC30S11-1510WT10×250 OC30S11-2510WT10×300 OC30S11-3010WT20×150 OC30S11-1520WT20×250 OC30S11-2520WT

SeeP148forpreparativecolumnsotherthanthoselistedabove.

Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 OC12S03-0102CC

4.0×23 OC12S03-G304CC

S-5 120 1.0×10 OC12S05-0101CC1.5×10 OC12S05-01P5CC2.0×10 OC12S05-0102CC4.0×23 OC12S05-G304CC

200 4.0×23 OC20S05-G304CC

300 1.0×10 OC30S05-0101CC1.5×10 OC30S05-01P5CC2.0×10 OC30S05-0102CC4.0×23 OC30S05-G304CC



XPCHSMW


XPCHW

Guard columnsParticle size（µm）

Pore size（Å）


S-5 120 4.0×10 OC12S05-0104WFG5.0×10 OC12S05-0105WFG10×30 OC12S05-0310WTG20×50 OC12S05-0520WTG

200 4.0×10 OC20S05-0104WFG20×50 OC20S05-0520WTG

300 4.0×10 OC30S05-0104WFG5.0×10 OC30S05-0105WFG10×30 OC30S05-0310WTG20×50 OC30S05-0520WTG

S-10 120 4.0×10 OC12S11-0104WFG5.0×10 OC12S11-0105WFG10×30 OC12S11-0310WTG20×50 OC12S11-0520WTG

200 20×50 OC20S11-0520WTG

300 4.0×10 OC30S11-0104WFG5.0×10 OC30S11-0105WFG10×30 OC30S11-0310WTG20×50 OC30S11-0520WTG


第02章.indd 65 11.1.28 10:46:47 AM

66

2

Reversed-P

hase Colum

ns (Other than O

DS

)

Analytical columns

YMC-Pack C4

● Stationary phase with low hydrophobicity● Different separation characteristics from ODS

R e v e r s e d - p h a s e c o l u m n w i t h s h o r t e r a l k y l c h a i nTheYMC-PackC4stationaryphasesurfacehydrophobicityislowerthanthatofbothODSandC8RetentiontimesofsamplesonYMC-PackC4thereforetendtobeshorterthanthoseonODSorC8.Separation

characteristicsofYMC-PackC4alsodifferfromthoseofODS.YMC-PackC4achievesbetterseparationthanODSforsometypesofsamples.

■Poresize：120Å,200Å,300Å■Carboncontent：7％ ,5％ ,3％■UsablepHrange：2.0～ 7.5

A p p l i c a t i o n （T920302A）

1

2

34

5

6

7

8910

11

12

50 10 15 20 25 min

2,4-DNPH derivatives of aldehydes and ketones

Column ：YMC-PackC4(5µm,120Å) 150×4.6mmI.D.Eluent ：A)tetrahydrofuran/water(10/90) B)acetonitrile 35％B(0-7min),35-65％B(7-18min,linear), 100％B(18-19min),35％B(19-35min)Flowrate ：1.5mL/minTemperature：30℃Detection ：UVat360nm

1. Formaldehyde 2,4-DNPH 2. Acetaldehyde 2,4-DNPH 3. Acetone 2,4-DNPH 4. Acrolein 2,4-DNPH 5. Propionaldehyde, 2,4-DNPH 6. Crotonaldehyde 2,4-DNPH 7. Methylethylketone 2,4-DNPH 8. Isobutyraldehyde 2,4-DNPH 9. Benzaldehyde 2,4-DNPH 10. n-Valeraldehyde 2,4-DNPH 11. p-Tolualdehyde 2,4-DNPH 12. Capronaldehyde 2,4-DNPH



Pore size（Å）


S-3 120 2.0× 35 BU12S03-H502WT2.0× 50 BU12S03-0502WT2.0× 75 BU12S03-L502WT2.0×100 BU12S03-1002WT2.0×150 BU12S03-1502WT3.0× 50 BU12S03-0503WT3.0×100 BU12S03-1003WT3.0×150 BU12S03-1503WT4.6×100 BU12S03-1046WT4.6×150 BU12S03-1546WT

S-5 120 1.0×150 BU12S05-1501WT1.0×250 BU12S05-2501WT1.5×150 BU12S05-15P5WT1.5×250 BU12S05-25P5WT2.0×150 BU12S05-1502WT 2.0×250 BU12S05-2502WT4.6× 75 BU12S05-L546WT4.6×100 BU12S05-1046WT4.6×150 BU12S05-1546WT4.6×250 BU12S05-2546WT4.6×300 BU12S05-3046WT6.0×100 BU12S05-1006WT6.0×150 BU12S05-1506WT6.0×250 BU12S05-2506WT6.0×300 BU12S05-3006WT

200 4.6×150 BU20S05-1546WT4.6×250 BU20S05-2546WT

300 1.0×150 BU30S05-1501WT1.0×250 BU30S05-2501WT


Pore size（Å）


S-5 300 1.5×150 BU30S05-15P5WT1.5×250 BU30S05-25P5WT2.0×150 BU30S05-1502WT2.0×250 BU30S05-2502WT4.6× 75 BU30S05-L546WT4.6×100 BU30S05-1046WT4.6×150 BU30S05-1546WT4.6×250 BU30S05-2546WT4.6×300 BU30S05-3046WT6.0×100 BU30S05-1006WT6.0×150 BU30S05-1506WT6.0×250 BU30S05-2506WT6.0×300 BU30S05-3006WT

S-10 120 4.6×100 BU12S11-1046WT4.6×150 BU12S11-1546WT4.6×250 BU12S11-2546WT4.6×300 BU12S11-3046WT6.0×100 BU12S11-1006WT6.0×150 BU12S11-1506WT6.0×250 BU12S11-2506WT6.0×300 BU12S11-3006WT

300 4.6×100 BU30S11-1046WT4.6×150 BU30S11-1546WT4.6×250 BU30S11-2546WT4.6×300 BU30S11-3046WT6.0×100 BU30S11-1006WT6.0×150 BU30S11-1506WT6.0×250 BU30S11-2506WT6.0×300 BU30S11-3006WT

第02章.indd 66 11.1.28 10:46:48 AM

67

Reversed-P

hase Colum

ns (Other than O

DS

)

2


Pore size（Å）


S-5 120 10×150 BU12S05-1510WT10×250 BU12S05-2510WT10×300 BU12S05-3010WT20×100 BU12S05-1020WT20×150 BU12S05-1520WT20×250 BU12S05-2520WT

200 20×150 BU20S05-1520WT20×250 BU20S05-2520WT

300 10×150 BU30S05-1510WT10×250 BU30S05-2510WT10×300 BU30S05-3010WT20×150 BU30S05-1520WT20×250 BU30S05-2520WT

S-10 120 10×150 BU12S11-1510WT10×250 BU12S11-2510WT10×300 BU12S11-3010WT20×150 BU12S11-1520WT20×250 BU12S11-2520WT

200 20×150 BU20S11-1520WT20×250 BU20S11-2520WT

300 10×150 BU30S11-1510WT10×250 BU30S11-2510WT10×300 BU30S11-3010WT20×150 BU30S11-1520WT20×250 BU30S11-2520WT


Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 BU12S03-0102CC

4.0×23 BU12S03-G304CC

S-5 120 1.0×10 BU12S05-0101CC1.5×10 BU12S05-01P5CC2.0×10 BU12S05-0102CC4.0×23 BU12S05-G304CC

200 4.0×23 BU20S05-G304CC

300 1.0×10 BU30S05-0101CC1.5×10 BU30S05-01P5CC2.0×10 BU30S05-0102CC4.0×23 BU30S05-G304CC



XPCHSMW


XPCHW


Pore size（Å）


S-5 120 4.0×10 BU12S05-0104WFG5.0×10 BU12S05-0105WFG10×30 BU12S05-0310WTG20×50 BU12S05-0520WTG

200 4.0×10 BU20S05-0104WFG5.0×10 BU20S05-0105WFG20×50 BU20S05-0520WTG

300 4.0×10 BU30S05-0104WFG5.0×10 BU30S05-0105WFG10×30 BU30S05-0310WTG20×50 BU30S05-0520WTG

S-10 120 4.0×10 BU12S11-0104WFG5.0×10 BU12S11-0105WFG10×30 BU12S11-0310WTG20×50 BU12S11-0520WTG

200 20×50 BU20S11-0520WTG

300 4.0×10 BU30S11-0104WFG5.0×10 BU30S11-0105WFG10×30 BU30S11-0310WTG20×50 BU30S11-0520WTG


第02章.indd 67 11.1.28 10:46:49 AM

68

2

Reversed-P

hase Colum

ns (Other than O

DS

)

Analytical columns

YMC-Pack TMS● Stationary phase with the lowest hydrophobicity among reversed-phase packing

materials● Different separation characteristics from ODS

R e v e r s e d - p h a s e c o l u m n w i t h t h e l o w e s t h y d r o p h o b i c i t yYMC-PackTMSshowslowerretentionduetohydrophobicinteractionthanotherpackingmaterials,anditisusefulforelutinghighlyhydrophobiccompounds

inashorttime.Inaddition,itcansometimesachievegreaterretentionandbetterseparationofhydrophiliccompoundsthanotherreversed-phasecolumns.

■Poresize：120Å■Carboncontent：4％■UsablepHrange：2.0～ 7.5

Shorten analysis time using TMS

Column :50×2.0mmI.D.Eluent A)water/formicacid (100/0.05) B)acetonitrile/water/ formicacid(50/50/0.05)Flowrate :0.2mL/minTemperature:37℃Detection :ESIpositivemode

YMC-Pack Pro C18

Isocratic 36%B

5 10 15 20 25 30 min0

1

2

3

4

Gradient 30-90%B (0-10 min), 90%B (10-15 min)

5 10 15 min0

12

3

4

YMC-Pack Pro C18

YMC-Pack TMS

Isocratic 30%B

5 10 15 20 min0

1

2

3

4

Soy isoflavone

TMSenablesanalysistimeofhighlyhydrophobiccompoundstoshorten

1

2 34

100 20 min

Water-soluble vitamins1. p-Aminobenzoic acid (PABA)2. Biotin (Vitamin H)3. Riboflavin (Vitamin B2)4. Cyanocobalamin (Vitamin B12)

Column ：YMC-PackTMS(5µm,120Å) 150×4.6mmI.D.

Eluent ：acetonitrile/50mMNH4H2PO4(10/90)Flowrate ：1.0mL/minTemperature：37℃Detection ：UVat210nm

change column

change elution conditions

1.Daidzin2.Genistin3.Daidzein4.Genistein

A p p l i c a t i o n （M920212C）

第02章.indd 68 11.1.28 10:46:51 AM

69

Reversed-P

hase Colum

ns (Other than O

DS

)

2


Pore size（Å）


S-3 120 4.6×100 TM12S03-1046WT4.6×150 TM12S03-1546WT

S-5 120 1.0×150 TM12S05-1501WT1.0×250 TM12S05-2501WT1.5×150 TM12S05-15P5WT1.5×250 TM12S05-25P5WT2.0×150 TM12S05-1502WT2.0×250 TM12S05-2502WT4.6× 75 TM12S05-L546WT4.6×100 TM12S05-1046WT4.6×150 TM12S05-1546WT4.6×250 TM12S05-2546WT4.6×300 TM12S05-3046WT6.0×100 TM12S05-1006WT6.0×150 TM12S05-1506WT6.0×250 TM12S05-2506WT6.0×300 TM12S05-3006WT

S-10 120 4.6×100 TM12S11-1046WT4.6×150 TM12S11-1546WT4.6×250 TM12S11-2546WT4.6×300 TM12S11-3046WT6.0×100 TM12S11-1006WT6.0×150 TM12S11-1506WT6.0×250 TM12S11-2506WT6.0×300 TM12S11-3006WT


Pore size（Å）


S-5 120 10×150 TM12S05-1510WT10×250 TM12S05-2510WT10×300 TM12S05-3010WT20×100 TM12S05-1020WT20×150 TM12S05-1520WT20×250 TM12S05-2520WT

S-10 120 10×150 TM12S11-1510WT10×250 TM12S11-2510WT10×300 TM12S11-3010WT20×150 TM12S11-1520WT20×250 TM12S11-2520WT


Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)Particle size（µm）

Pore size（Å）


S-3 120 4.0×23 TM12S03-G304CC

S-5 120 1.0×10 TM12S05-0101CC1.5×10 TM12S05-01P5CC2.0×10 TM12S05-0102CC4.0×23 TM12S05-G304CC



XPCHSMW


XPCHW


Pore size（Å）


S-5 120 4.0×10 TM12S05-0104WFG5.0×10 TM12S05-0105WFG10×30 TM12S05-0310WTG20×50 TM12S05-0520WTG

S-10 120 4.0×10 TM12S11-0104WFG5.0×10 TM12S11-0105WFG10×30 TM12S11-0310WTG20×50 TM12S11-0520WTG


第02章.indd 69 11.1.28 10:46:52 AM

70

2

Reversed-P

hase Colum

ns (Other than O

DS

)

Analytical columns

YMC-Pack Ph● Reversed-phase column with π electrons● Unique selectivity due to π-π interaction● Useful in cases where separation optimization is difficult to achieve using ODS

D i f f e r e n t s e l e c t i v i t y f r o m O D SYMC-PackPhhasπelectronsofphenylgroup.YMC-PackPhshowsdifferentseparationcharacteristicsfromalkyl-silicastationaryphasesincludingODSforseparationofsolutessuchasaromaticcompounds,

sinceπ-πinteractionbetweenthestationaryphaseandsolutes,aswellashydrophobicinteraction,contributetotheseparation.

■Poresize：120Å■Carboncontent：9％■UsablepHrange：2.0～ 7.5

1. (-)-Epigallocatechin2. (+)-Catechin3. (-)-Epicatechin4. (-)-Epigallocatechin gallate5. (-)-Epicatechin gallate

Establishment of simple conditions using Ph

Column :150×4.6mmI.D.Flowrate :1.0mL/minTemperature:37℃Detection :UVat280nm

acetonitrile / water / TFA (12/88/0.1)

YMC-Pack Pro C18

1

3

5

2 4

N050622A

0 10 20 min

acetonitrile / ethyl acetate / water / TFA (9/3/88/0.1)

YMC-Pack Pro C18

2

34

5

1

N050620A

0 10 20 min

YMC-Pack Ph

acetonitrile / water / TFA (12/88/0.1)

2

53

1

4

N050623A

0 10 20 min

Catechins

Phissuitableforseparatingcatechinswithsimplesolvent,whereasifusingODSandoptimizinganalysisconditions,thesolventcomposition,additionwithaceticether,iscomplicated.

1

2

3

50 10 min

Aspartame in a commercially available carbonated beverage1. Benzoic acid2. Caffeine3. Aspartame

Column ：YMC-PackPh(5µm,120Å) 150×4.6mmI.D.

Eluent ：50mMKH2PO4/acetonitrile/methanol(84/3/13）Flowrate ：1.5mL/minTemperature：30℃Detection ：UVat214nm

change column

change solvent

A p p l i c a t i o n （T910123）

第02章.indd 70 11.1.28 10:46:55 AM

71

Reversed-P

hase Colum

ns (Other than O

DS

)

2


Pore size（Å）


S-3 120 2.0× 35 PH12S03-H502WT2.0× 50 PH12S03-0502WT2.0× 75 PH12S03-L502WT2.0×100 PH12S03-1002WT2.0×150 PH12S03-1502WT3.0× 50 PH12S03-0503WT3.0×100 PH12S03-1003WT3.0×150 PH12S03-1503WT4.6×100 PH12S03-1046WT4.6×150 PH12S03-1546WT

S-5 120 1.0×150 PH12S05-1501WT1.0×250 PH12S05-2501WT1.5×150 PH12S05-15P5WT1.5×250 PH12S05-25P5WT2.0×150 PH12S05-1502WT2.0×250 PH12S05-2502WT4.6× 75 PH12S05-L546WT4.6×100 PH12S05-1046WT4.6×150 PH12S05-1546WT4.6×250 PH12S05-2546WT4.6×300 PH12S05-3046WT6.0×100 PH12S05-1006WT6.0×150 PH12S05-1506WT6.0×250 PH12S05-2506WT6.0×300 PH12S05-3006WT

S-10 120 4.6×100 PH12S11-1046WT4.6×150 PH12S11-1546WT4.6×250 PH12S11-2546WT4.6×300 PH12S11-3046WT6.0×100 PH12S11-1006WT6.0×150 PH12S11-1506WT6.0×250 PH12S11-2506WT6.0×300 PH12S11-3006WT


Pore size（Å）


S-5 120 10×150 PH12S05-1510WT10×250 PH12S05-2510WT10×300 PH12S05-3010WT20×100 PH12S05-1020WT20×150 PH12S05-1520WT20×250 PH12S05-2520WT

S-10 120 10×150 PH12S11-1510WT10×250 PH12S11-2510WT10×300 PH12S11-3010WT20×150 PH12S11-1520WT20×250 PH12S11-2520WT



Pore size（Å）


S-3 120 2.0×10 PH12S03-0102CC4.0×23 PH12S03-G304CC

S-5 120 1.0×10 PH12S05-0101CC1.5×10 PH12S05-01P5CC2.0×10 PH12S05-0102CC4.0×23 PH12S05-G304CC

Acartridgeholderwillneedtobepurchasedseparatelybeforeusingthisproductforthefirsttime.


XPCHSMW


XPCHW


Pore size（Å）


S-5 120 4.0×10 PH12S05-0104WFG5.0×10 PH12S05-0105WFG10×30 PH12S05-0310WTG20×50 PH12S05-0520WTG

S-10 120 4.0×10 PH12S11-0104WFG5.0×10 PH12S11-0105WFG10×30 PH12S11-0310WTG20×50 PH12S11-0520WTG


第02章.indd 71 11.1.28 10:46:56 AM

72

2

Reversed-P

hase Colum

ns (Other than O

DS

)

Analytical columns

YMC-Pack CN● Normal-phase and reversed-phase modes are selectable according to the purpose

of analysis● Low hydrophobicity● Unique selectivity due to cyano group

Column can be used in both normal -phase and reversed -phase modesYMC-PackCNcanbeusedinbothnormal-phaseandreversed-phasemodes,sinceithascyanopropylgroupofmediumpolaritychemicallybondedtothestationaryphase.Itcanbeusedinnormalphasemodewithlow-polaritymobilephasesuchashexane.Itcanalsobeusedinreversed-phasemodewithhighly-polarmobilephasesuchasmethanolandwater.

ThehydrophobicityofYMC-PackCNisrelativelylowforareversed-phasepackingmaterial,anditshowsdifferentselectivityfromODSduetoπelectronsofthecyanogroups.YMC-PackCNisusefulforshorteninganalysistimewhenretentiontimeistoolongwithODSandusefulincaseswhereseparationoptimizationisdifficulttoachieveusingODS.

■Poresize：120Å,300Å■Carboncontent：7％ ,3％■UsablepHrange：2.0～ 7.5

10 20 min

1

4

32

0

Anti-hyperlipidemia drugs

CI O C COOHCONHCH CH2

CH

CH3

3

2

1.

Bezafibrate

CI O HC COOC

CH CH

CHCH3

3

2.

Clofibrate

52 O ( CH ) C COOH

CH

CH3

33

3

3.

Gemfibrozil

2 3

CI CO O C COOCH

CH

CH

CH

CH3

3

3

3

4.

Fenofibrate

Column ：YMC-PackCN(5µm,120Å) 150×4.6mmI.D.

Eluent ：acetonitrile/methanol/water/aceticacid(20/20/60/1)Flowrate ：1.0mL/minTemperature：37℃Detection ：UVat275nm

A p p l i c a t i o n （S931025E）


Pore size（Å）


S-3 120 2.0× 35 CN12S03-H502WT2.0× 50 CN12S03-0502WT2.0× 75 CN12S03-L502WT2.0×100 CN12S03-1002WT2.0×150 CN12S03-1502WT3.0× 50 CN12S03-0503WT3.0×100 CN12S03-1003WT3.0×150 CN12S03-1503WT4.6×100 CN12S03-1046WT4.6×150 CN12S03-1546WT

S-5 120 1.0×150 CN12S05-1501WT1.0×250 CN12S05-2501WT1.5×150 CN12S05-15P5WT1.5×250 CN12S05-25P5WT2.0×150 CN12S05-1502WT2.0×250 CN12S05-2502WT4.6× 75 CN12S05-L546WT4.6×100 CN12S05-1046WT4.6×150 CN12S05-1546WT4.6×250 CN12S05-2546WT4.6×300 CN12S05-3046WT6.0×100 CN12S05-1006WT6.0×150 CN12S05-1506WT6.0×250 CN12S05-2506WT6.0×300 CN12S05-3006WT


Pore size（Å）


S-5 300 1.0×150 CN30S05-1501WT1.0×250 CN30S05-2501WT1.5×150 CN30S05-15P5WT1.5×250 CN30S05-25P5WT2.0×150 CN30S05-1502WT2.0×250 CN30S05-2502WT4.6× 75 CN30S05-L546WT4.6×100 CN30S05-1046WT4.6×150 CN30S05-1546WT4.6×250 CN30S05-2546WT4.6×300 CN30S05-3046WT6.0×100 CN30S05-1006WT6.0×150 CN30S05-1506WT6.0×250 CN30S05-2506WT6.0×300 CN30S05-3006WT

S-10 120 4.6×100 CN12S11-1046WT4.6×150 CN12S11-1546WT4.6×250 CN12S11-2546WT4.6×300 CN12S11-3046WT6.0×100 CN12S11-1006WT6.0×150 CN12S11-1506WT6.0×250 CN12S11-2506WT6.0×300 CN12S11-3006WT


第02章.indd 72 11.1.28 10:46:58 AM

73

Reversed-P

hase Colum

ns (Other than O

DS

)

2


Pore size（Å）


S-5 120 10×150 CN12S05-1510WT10×250 CN12S05-2510WT10×300 CN12S05-3010WT20×100 CN12S05-1020WT20×150 CN12S05-1520WT20×250 CN12S05-2520WT

300 20×150 CN30S05-1520WT20×250 CN30S05-2520WT

S-10 120 10×150 CN12S11-1510WT10×250 CN12S11-2510WT10×300 CN12S11-3010WT20×150 CN12S11-1520WT20×250 CN12S11-2520WT


Guard cartridge columns (with inner diameter 1.0, 1.5, or 2.0 mm: 2/pack, 4.0 mm: 3/pack)S-3 120 2.0×10 CN12S03-0102CC

4.0×23 CN12S03-G304CC

S-5 120 1.0×10 CN12S05-0101CC1.5×10 CN12S05-01P5CC2.0×10 CN12S05-0102CC4.0×23 CN12S05-G304CC

300 1.0×10 CN30S05-0101CC1.5×10 CN30S05-01P5CC2.0×10 CN30S05-0102CC4.0×23 CN30S05-G304CC



XPCHSMW


XPCHW


Pore size（Å）


S-5 120 4.0×10 CN12S05-0104WFG5.0×10 CN12S05-0105WFG10×30 CN12S05-0310WTG20×50 CN12S05-0520WTG

300 4.0×10 CN30S05-0104WFG5.0×10 CN30S05-0105WFG10×30 CN30S05-0310WTG20×50 CN30S05-0520WTG

S-10 120 4.0×10 CN12S11-0104WFG5.0×10 CN12S11-0105WFG10×30 CN12S11-0310WTG20×50 CN12S11-0520WTG


第02章.indd 73 11.1.28 10:46:58 AM

74

2

Reversed-P

hase Colum

ns (Other than O

DS

)

Analytical columns

YMC-Pack PROTEIN-RP● Improved recovery of proteins or peptides● Improved durability when used with TFA solution● Enables elution of high molecular weight proteins

R e v e r s e d - p h a s e c o l u m n f o r s e p a r a t i o n o f p r o t e i n s o r p e p t i d e sYMC-PackPROTEIN-RPisareversed-phasecolumnutilizingasilicagelbase.Itcontainsastationaryphase,specificallydesignedforseparationofproteinsorpeptides.Problemsthatareassociatedwithconventional

reversed-phasecolumnswithshortalkylchainlengthsareminimized.Robustcolumnlifetimeandexcellentrecoveryofhydrophobicproteinsaretypicallypossiblewiththisphase.

■Carboncontent：4％■UsablepHrange：1.5～ 7.5

Improved recovery of proteins or peptides

Recoveryofvariousproteinsandpeptidesareshown.YMC-PackPROTEIN-RPgivesgreaterrecoverythancompetitorC4columns.

recovery of proteins or peptides

SampleRecovery（％）

YMC-Pack PROTEIN-RP

C4 column,company A

C4 column,company B

Ribonuclease A 93 95 86Cytochrome c 94 89 95Lysozyme 98 93 76Myoglobin 97 85 93BSA 94 92 86Ovalbumin 90 73 88Transferrin 94 98 88Insulin(bovine) 97 73 92Insulin B chain 82 76 70α-Mating factor 93 82 81Leu-Enkephalin 92 84 91Gly-Gly-Gly-Gly 95 86 70

Improved durability when used with TFA solution

110

100

90

80

70

60

50

40

30

20

10

0100 200 300 400

C4 column, Company B

C4 column, Company A

（Peak split）

YMC-Pack PROTEIN-RP

×

Solvent flow in hours

0.1%TFA condition

% retention of diphenyl

1. Uracil2. Benzene3. Naphthalene4. Diphenyl

〈Flowconditions〉Eluent ：water/TFA(100/0.1)Flowrate ：1.0mL/minTemperature：ambient

〈Measurementconditions〉Column ：YMC-PackPROTEIN-RP

250×4.6mmI.D.Eluent ：acetonitrile/water(40/60)Flowrate ：1.0mL/minTemperature：30℃Detection ：UVat254nm,0.32AUFS

Testresultsofthestabilityofstationaryphasewith0.1%aqueousTFAisshownontheleft.RetentionofdiphenylonC4columnsmanufacturedbyothercompaniesgreatlydecreasesastimepasses.Thisiscausedbycleavageofbutylgroupsfromthepackingmaterialduetoacidhydrolysis.RetentionofdiphenylonYMC-PackPROTEIN-RPisshowntobestableafter500hoursofmobilephaseflow.

〈Prior to flow〉1

2

3

4

0 5 10 min

Theoretical plate number (4) = 14,300

〈After 500 hours〉

1

2

3

4

0 5 10 min

Theoretical plate number (4) = 13,800

第02章.indd 74 11.1.28 10:47:01 AM

75

Reversed-P

hase Colum

ns (Other than O

DS

)

2

0 10 20 30 min

12

3

Proteins1. Cytochrome c (MW:12,400, 5 µg)2. BSA (MW:66,000, 25 µg)3. Ferritin (MW:440,000, 10 µg)

Column ：YMC-PackPROTEIN-RP250×4.6mmI.D.

Eluent ：A)water/TFA(100/0.1) B)acetonitrile/TFA(100/0.1) 30-90％B(0-45min,linear)

Flowrate ：1.0mL/minTemperature：ambientDetection ：UVat280nm,0.04AUFS

A p p l i c a t i o n

1

23 4 5

67

8

9

1011

12

13

403020100 min

Proteins and peptides

Column ：YMC-PackPROTEIN-RP 250×4.6mmI.D.

Eluent ：A)water/TFA(100/0.1) B)acetonitrile/TFA(100/0.1) 10-90％B(0-60min,linear)

Flowrate ：1.0mL/minTemperature：ambientDetection ：UVat220nm,0.32AUFS

1. Met-Enkephalin2. Leu-Enkephalin3. Oxytocin4. Bradykinin5. Angiotensin l6. Ribonuclease A7. α-Mating factor

8. Insulin(bovine)9. Cytochrome c

10. Lysozyme11. BSA12. β-Lactoglobulin13. Ovalbumin




S-5 2.0×150 PR99S05-1502WT2.0×250 PR99S05-2502WT4.6×150 PR99S05-1546WT4.6×250 PR99S05-2546WT

Semi-preparative columnsS-5 10×250 PR99S05-2510WT

20×150 PR99S05-1520WT20×250 PR99S05-2520WT

Guard cartridge columns (three-pack)S-5 4.0×23 PR99S05-G304CC


Cartridge holderCartridge holder(inner diameter: 4.0 mm)

XPCHW



S-5 4.0×10 PR99S05-0104WFG10×30 PR99S05-0310WTG20×50 PR99S05-0520WTG


第02章.indd 75 11.1.28 10:47:02 AM

76

2

Reversed-P

hase Colum

ns (Other than O

DS

)

Analytical columns

YMCbasic● Superior separation of basic compounds● Useful for separation of peptides● Secondary interaction minimized as much as possible

C o l u m n f o r s e p a r a t i o n o f b a s i c c o m p o u n d sYMCbasicisareversed-phasesilicabasedC8columndesignedforseparationofbasiccompounds,includingpharmaceuticalproducts.Itishighlyevaluatedasa"neutral"silanolgroupinEuropeandtheU.S.Itoffers

superiorseparationofacidiccompoundsaswellasbasiccompounds.Itissuitableforseparatingpeptideswithmolecularweightsintherangeofseveralthousands,suchasinsulin.

■Carboncontent：7％■UsablepHrange：2.0～ 7.5

0 10 20 30 40 50

0

20

40

60mAU

60 min

*

* undigested BSA

Tryptic digest of BSA

Column ：YMCbasic(5µm) 150×2.0mmI.D.

Eluent ：A)water/TFA(100/0.1) B)acetonitrile/TFA(100/0.1) 5-35% B(0-50min),35-45% B(50-55min), 45% B(55-60min)Flowrate ：0.2mL/minTemperature：37℃Detection ：UVat220nm




Pore size（Å）


S-3 200 2.0× 35 BA99S03-H502WT2.0× 50 BA99S03-0502WT2.0× 75 BA99S03-L502WT2.0×100 BA99S03-1002WT2.0×150 BA99S03-1502WT3.0× 50 BA99S03-0503WT3.0×100 BA99S03-1003WT3.0×150 BA99S03-1503WT4.6× 50 BA99S03-0546WT4.6×100 BA99S03-1046WT4.6×150 BA99S03-1546WT

S-5 200 2.0×150 BA99S05-1502WT3.0×150 BA99S05-1503WT4.6× 50 BA99S05-0546WT4.6×100 BA99S05-1046WT4.6×150 BA99S05-1546WT4.6×250 BA99S05-2546WT6.0×150 BA99S05-1506WT6.0×250 BA99S05-2506WT


Pore size（Å）


S-3 200 4.0×23 BA99S03-G304CC

S-5 200 2.0×10 BA99S05-0102CC4.0×23 BA99S05-G304CC



XPCHSMW


XPCHW

第02章.indd 76 11.1.28 10:47:03 AM

Reversed-P

hase Colum

ns (Other than O

DS

)

2

77

Analytical columns

YMC Carotenoid● Resolves polar and nonpolar geometric carotenoid isomers● Separates carotenoids in blood samples, food products, natural product extracts, and

commercial preparations● Operates with low aqueous or non aqueous mobile phases desirable in LC/MS and prep fraction recovery

C a r o t e n o i d a n a l y t i c a l c o l u m nYMCCarotenoidisC30bondedsilicabasedreversedphasecolumn.Itisforcarotenoidanalysisandusefulforseparationofgeometricisomers.

■UsablepHrange：2.0～ 7.5

0 10 20 30 40 50 60 70 80

1

23

4

5

6

7

8

9

10

11

12

13 14

min

Carotene and xanthophyll

Column ：YMCCarotenoid 250×4.6mmI.D.

Eluent ：A)methanol/MTBE＊/H2O(81/15/4)B)methanol/MTBE＊/H2O(6/90/4)0-100%B(0-90min)

Flowrate ：1.0mL/minTemperature：ambientDetection ：UVat450nm＊methyltert-butylether

1. Astaxanthin2. Capsanthin3. Lutein4. Zeaxanthin5. Canthaxanthin6. β-Cryptoxanthin7. Echinenone8. 15-cis β-Carotene9. 13-cis β-Carotene

10. α-Carotene11. trans β-Carotene12. 9-cis β-Carotene13. δ-Carotene14. Lycopene



Analytical and Semi-preparative columnsParticle size（µm）


S-3 4.6×100 CT99S03-1046WT4.6×150 CT99S03-1546WT

S-5 4.6×150 CT99S05-1546WT4.6×250 CT99S05-2546WT20×150 CT99S05-1520WT20×250 CT99S05-2520WT

Guard cartridge columns (three-pack)Particle size（µm）


S-3 4.0×23 CT99S03-G304CC

S-5 4.0×23 CT99S05-G304CC


Cartridge holderCartridge holder(inner diameter: 4.0 mm)

XPCHW

第02章.indd 77 11.1.28 10:47:04 AM

第02章.indd 78 11.1.28 10:47:05 AM

reversed-phase columns (other than ods) · pdf fileymc-pack series include a variety of...

Documents