restriction enzymes
DESCRIPTION
Restriction EnzymesTRANSCRIPT
Describe digestion of DNA by
LearningOutcomes:1.DefineandGiveexamplesofRestrictionEndonucleases.
2.ExplainhowrestrictionenzymesproduceRecombinantDNA.
DNA Manipulation:
Enzymescalledrestrictionendonucleasesrevolutionisedmolecularbiologybecauseoftheirabilityto“cut”DNAatspecificsites.
ButHowDOrestrictionenzymeswork?
Theyallowthecreationofrecombinantmolecules;theseenzymesrecogniseaspecificDNAsequence,rangingfromandcuttheDNAataspecificbasewithinthissequence.
TheRecognitionsitesformanyRestrictionEnzymesarepalindromes...
Apalindromeisawordorphrasethatreadsthesameforwardandinreverse,e.g...
SO!...ThepalindromicDNAsequencereadsthesamefrom5'to3'ononestrand
asitdoesonthecomplementarystrand.
CuttingtheDNAatthesamebaseoneitherstrandproducesstaggeredcutsCalled“stickyends.”
SO,These“stickyends”allowDNAfromdifferentsourcestobeeasilyjoinedtogether!
Theseshort,unpairedsequencesarethesameforanyDNAthatiscutbythisenzyme...
Describe this Diagram!! Work in pairs, You have 5 minutes to provide a step by step, explanation of what it is going on! (use the animation to help!)