RESTRICTION ENZYME ANALYSIS

IMBB 2013

RE: recap

NEBcutter-online tool (free)

• This tool will take a DNA sequence and find the

largest, non-overlapping ORF.

• Can select restriction enzymes that cut the

sequence from a wide range of commercial RE.

• Can customize restriction digestion.

• Can visualize restriction fragments on a virtual

agarose gel.

• http://tools.neb.com/NEBcutter2/

RFs