Functional Analysis of

SOYBEAN LEC1Hala Addassi

Undergraduate Harada Lab

Seed Development

LEC1 expression in Soybean

EP EP-AX EP-COT ESSUS Seed Coat sub-regions

Glyma17g00950

Glyma07g39820

Glyma20g00240

Glyma03g18670

Arabidopsis

Research Question

Does Soybean LEC1 have a similar function to Arabidopsis

LEC1?

LEC1 is a Central Regulator of Seed Development

DesiccationTolerance

wt

lec1 mutant

DesiccationTolerance

DesiccationTolerance

Embryo Rescue

LEC1 regulates transition from embryogenesis to postembryonic development

Approach Step 1

LEC1 CASSETTE LEC1 CASSETTE SOYBEAN LEC1

CONSTRUCT

Generate Construct for SOYBEAN LEC1

Transformed the plants by agrobacterium infiltration

LEC1 GFPLEC1 CASSETTE LEC1 CASSETTE

CONTROL

LEC1 CASSETTE LEC1 CASSETTE SOYBEAN LEC1

CONSTRUCT

1. ARABIDOPSIS LEC 1 in lec 1 mutant

2. ARABIDOPSIS LEC 1 in WS-O (Wild Type)

3. SOYBEAN LEC 1 in lec 1 mutant

4. SOYBEAN LEC 1 in WS-O (Wild Type)

Approach Step 2

Results

Before BASTA After BASTA

Summary

▪Transformation of SOYBEAN LEC1 in WS-O most likely contains the transgene.

▪Currently, I am trying to confirm the presence of the transgene.

Future Plans

▪Complete agrobacterium transformation with 3 other lines.

▪ARABIDOPSIS LEC1 in WS-O

▪ARABIDOPSIS LEC1 in lec 1 mutant

▪SOYBEAN LEC1 in lec 1 mutant

▪ Screen the T1 plants to see if soybean LEC1 gene can complement for Arabidopsis lec1 mutant plants