Presented at The 2nd INTERNATIONAL WORKSHOP ON SATOUMI-GEMPITA SPL-GAPURA

Pekalongan,Central Java, Indonesia, November 26, 2014

RESEARCH ACTIVITIES ON THE DEVELOPMENT OF

MARINE RESOURCES BASED FOOD AND HEALTH PRODUCTS

IN BPPT

Dr. Ir. Listyani WijayantiDeputy Chairman BPPT for Agroindustry and Biotechnology

MARINE AFFAIRS AND FISHERIES IN INDONESIA

• LARGEST ARCHIPELAGO IN THE WORLD, More Than 17.000 Islands

• 2/3 OF ITS TERRITORY IS SEA, 81.000 km Coast Line

• HIGHLY DEPEND ON THE SEA

Nawacita (Nine Priorities Agenda)• Bringing back the country for warranty on national security and defence

• Promoting a clean, effective, democratic and trusted governance

• Development of regional and villages area in the frame of a unitary state

• System reformation and corruption free, dignified and trustworthy law enforcement

• Enhancing the life quality of Indonesian people

• Increase productivity and competitiveness in the international market

• Promoting self-reliance economy by driving domestic economy strategic sectors

• Character revolution of the nation

• Affirming the nation’s diversity and strengthening social restoration

MARINE AFFAIRS AND FISHERIES IN INDONESIA

National Long Term Development Plan (RPJPN) 2005-2025:

1) National competitiveness where fishery sector is a primemover for food security and national economic growth

2) National self-reliances based on Indonesia as an archipelagonational interest by securing marine and fishery resources anddeveloping optimal and sustainable marine and fishery industry

• ACT NO. 32 ON MARINE WAS VOTED FOR IN SEPTEMBER 2014

• ACT NO. 45/2009 ON FISHERIES

MARINE BIOTECHNOLOGY RESEARCH

Development of Marine Biotechnology as written in the Act No 32 on Marine (article 25th and 26th)

FOOD

HEALTH

ENERGY

ENVIRONMENT

INDUSTRIAL PRODUCT & PROCESS

Aim of Marine Biotechnology Industry :(as written in Act No. 32/2914 on marine Affairs)

• 1. To prevent from the extinction of marine biota due to over exploration

• 2. To generate various added-value product

• 3. To reduce dependency on import goods by production of import- substitute products

• 4. To develop environmentally friendly technology in marine biotechnology industry

• 5. To develop a system for a sustainable management of marine bio-resources

TARGET RESEARCH AREA• Genomics and Molecular Biology

• Cultivation of Marine organisms

• Bio-engineering of Marine Micro-organisms

• Marine Model Organisms

MARINE BIODIVERSITY OF INDONESIA

• POTENCY :

More than 415 Fish Species; 13 Seagrass,682 Species of Seaweed, 2500 Species ofCrustacea, 745 Species of Echinodermata; etc.

POTENCY FOR FOOD• FISH PRODUCT HAS CONTRIBUTED AS MUCH AS 72%

AS SOURCES OF ANIMAL PROTEIN

COMPARE TO POULTRY (56,98%) AND BEEF (16,18%)

• RANK 5th AS EXPORT COMMODITY

• INCREASING FISH CONSUMPTION 2015 : 40,09 Kg/capita/year (5,5 % increase per year)

• TOTAL FISH CONSUMPTION DEMAND 2015 : 10,3 Million Ton (6,5 % increase per year)

POTENCY FOR HEALTH INDUSTRY• Utilization of sea for pharmaceutical and cosmetics industry has not been

yet optimal. In fact, the potential for marine based pharmaceutical andcosmetic products reaches 9 Billion US$ or equivalent to Rp 81.000 Trillionper year

• World demand for drugs increases by 20-30 percent per year. World HealthOrganization (WHO) has encouraged the development of the pharmaceuticalindustry. If marine pharmaceutical industry is well running, the economicvalue generated could reached 4-5 times of the overall economic potentialof fisheries,

• The utilization of potency for the pharmaceutical and cosmetics industry iscurrently only 10 percent.

TECHNOLOGY PRODUCTION OF GROUPER FISH(Humpback & Tiger Grouper)

Genetic Improvement of Humpback Grouper- Production super growth Humpback grouperMethod : transgenesis

Fish Disease Preventiona. Production of Vibrio Vaccine

Method : inactivation of Vibrio spp. bacteria b. Production of VNN Vaccine

Method: inactivation of Viral Necrosis Nervous c. Identification and characterization of pathogen,

Method : molecular marker using 16SrRNA

Fish Feed and Nutrition

• Production feed for growing out and brood stock• Development of feed additive Protein

Recombinant Growth Hormone (rGH)

PRODUCTION TECHNOLOGY OF SALINA (Saline Indonesian Tilapia)

• Salina can tolerate salinity until 20 ppt

• To be cultured in Brackish water pond

PRODUCTION TECHNOLOGY OF MARINE TILAPIA

• Next step, Salina Tilapia is to be cultured in sea waters (salinity 32 ppt)• Solution for the limitation of freshwater and inland fish culture area• The fillet of marine tilapia can substitute seabass fillet’s

One of the problem in aquacultureincluding tillapia farming is the highcost of feed which is nearly 65% ofthe total production.

To overcome this problem, it isnecessary to develop a technologicalbreakthrough and innovation in thefeed production. BPPT develops afeed additive: Growth HormoneRecombinant Protein (rGH).Feed additive Recombinant Proteins Growth

Hormone is very useful to accelerate the growthof fish including tilapia and developed through theapplication of recombination proteins that canproduce recombinant growth hormone in largeamounts by E. coli

DEVELOPING FEED ADDITIVES TECHNOLOGY: GROWTH HORMONE

RECOMBINANT PROTEINS (rGH)

To increase security in aquaculture

and reduce imported vaccine

products, BPPT develops DNA

Vaccines Streptococcus production.

DNA vaccines have advantages over

conventional vaccines that are

generic, safe and poses no risk of

infectious disease.

To produce the DNA Vaccine

Streptococcus, the bacteria S. inniae

isolated from organs of infected

tilapia. Then the gene was isolated

and cloned virulent streptococcus in

the expression vector and

transformed into E. coli to produce

plasmid DNA as a DNA vaccine

Streptococcus DNA Vaccine to Prevent Disease Outbreak in Tilapia Aquaculture

Integrated Multi Tropic Aquaculture

Bio-recycling System culture

Integrated Poly Culture among Sea weed as Environment buffer; Green Mussel as filter feeder; Tilapia fish (Nila Salina) and Black tiger prawn (Penaeus monodon)

DEVELOPMENT OF DEVICES FOR TRACEABILITY OF AQUACULTURE PRODUCT

Background

▪ Fundamental traceability information is required to identify the product and tracesits physical movement through the supply chain

▪ BPPT developed a Specifically Required Information to be recorded at anappropriate stages of the aquaculture product process and supply chainCommercially Desirable Information about the origin of the product and thecircumstances of its production that is recorded by finfish/shellfish aquaculturecompanies is needed for a variety of reasons

▪ Part of a program for elevating global competitiveness of aquaculture productsfrom Indonesia

Activity

- Developing traceability system to monitor, maintain, make plots, and verify security,nutrition and quality of Indonesian fishery products;

- Developing traceability entity tool kits (seed, feed, nutrition, processed products) tosupport the manufacture of Indonesian fishery product traceability systems;

- Developing traceability unit model for all stages from production, harvesting,transportation and to processing

Wireless barcode scanner barcode printer

Computer

DEVELOPMENT OF DEVICES FOR TRACEABILITY OF AQUACULTURE PRODUCT

High Export Standard (Grade AA) :

1. Gel Strength : 457,82 gr.cm2. Whiteness Degree : 59,94% 3. Crude Fat : 0,44%

In comply with standard of PT. Global Seafood International Indonesia (GSII)

SALINA AND MARINE TILAPIA PRODUCT POTENTIAL AS RAW MATERIAL OF SURIMI

TILAPIA FISHWHOLE BODY

TILAPIA FISHFILLET

TILAPIA FISHSURIMI

PRODUCTION TECHNOLOGY OF FISH GELATIN

Fish Fillet Extraction Evaporation Drying & Printing

Fish Gelatin Product

Searching of active compounds from marine organisms (Sponges,

Marine microbes and Microbial Symbiosis in the sponge)

Applied Research Activities :• Isolation and Fermentation of Marine Microbial• Screening activities of some Marine Microbial

Extracts• Isolation of active compounds, structure

elucidation and bioassay

MARINE RESOURCES RESEARCH FOR HEALTH

Fish Oil is susceptible to oxidation and unpleasant fishy taste

Difficult to purify DHA like compounds

DHA content << 7-14 % DHA content is high over fishing dterimental to the ecosystem

-

Other source of DHA should be found

MICROALGAE

Crypthecodinium cohnii, Ulkeniaesp. Thraustochytrium aureum andSchizochytrium sp isolated from mangrove forests in Indonesia

Development Polyunsaturated Fatty Acid (PUFA) Production From Microalgae

Technology Innovation on Pharmaceutical Salts Production for

Self Reliance on Pharmaceutical Raw Materials

Patent has been granted to BPPT on theproduction of pharmaceutical salt(Pharmaceutical Grade).The technology has been commercialized viacooperation with PT. Kimia Farma (PerseroTbk, a large pharmaceutical state-ownedenterprise.The pharmaceutical salt plant (cap. 2000 tons)is going to be built in Watudakon, East JavaTechnology transfer and production processesare expected by mid 2015.

The success of this pharmaceutical salt plantwill have an impact on reducing imports ofpharmaceutical salts and trigger thedevelopment of other pharmaceutical rawmaterials industryies