reprogramming of differentiated cells into stem cells or other somatic cells
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Reprogramming of differentiated cells into stem cells or other somatic cells. Content. Basics Stem cells Transcription factors Reprogramming of differentiated cells Method of reprogramming into a pluripotent stem cells - PowerPoint PPT PresentationTRANSCRIPT
Reprogramming of differentiated cells into stem cells or other
somatic cells
Content1. Basics 2. Stem cells3. Transcription factors4. Reprogramming of differentiated cells5. Method of reprogramming into a pluripotent stem cells6. Reprogramming of cells into multipotent stem cells7. Direct reprogramming into another somatic cell8. Alternative methods of reprogramming9. Vector systems 10. Possible areas of use for this methods
Basics
• Stem cells
• Transcriptionfactors
Aktivator
Bindung an Enhancer
Verstärkung der Transkription
Bindung an Operator
Stabilisation der RNS-
Polymerase
Bindung an Coaktivator
Verstärkung der Transkription
Katalysation der Acetylierung
Offenere DNS = Hochregulation
Repressor
Bindung an Operator
Verhindern der Transkription
Bindung an Corepressor
Abregulierung der Transkription
Katalysieren der Deacetylierung
Engere DNS = Verminderung
der Transkription
Bindung an einen Silencer
Herabregulation der Transkription
Reprogramming of differentiated cells
• Method of reprogramming into a pluripotent stem cells
• Reprogramming of cells into multipotent stem cells
Transkriptionsfaktoren
Somatische Zelle
Multipotente Zelle
Zellen eines bestimmten Keimblattes
• Direct reprogramming into another somatic cell
Transkriptionsfaktoren
Somatische Zelle
Somatische Zelle des eigenen Keimblattes
Somatische Zelle eines anderen Keimblattes
• Alternative methods of reprogramming
TranskriptionsfaktorenTranskriptionsfaktoren +
Chemische Verbindungen
mikro-RNS
Somatische Zellen
Pluripotente Stammzelle
Zellen der drei Keimblätter
Zellen der Keimbahn
• Vector systems
Retrovirus
Lentivirus
• Possible areas of use for this methods
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