refining margarine crypthecodinium cohnii: a natural dha factory 20 % lipid content by weight which...
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Refining Margarine
Crypthecodinium cohnii:A natural DHA Factory
•20 % lipid content by weight which is 40 % DHA•Facultative heterotroph, which does not perform
photosynthesis •Growth rate of 0.0375 h-1 on glucose substrate•Uses glucose as a carbon source •Uses dead yeast from ethanol bottoms as a nitrogen
source•Requires dissolved oxygen for optimal growth•Grows in brackish (saline) water.
Fermentation
Travis Dallas Eric Graves Joaquin Martinez Chris McNinch Ramune O. Meskyte Charu SainiMentors: Dan Rusinak, Jeffery Perl, John Micheli
•Lack of DHA is associated with poor or slower retinal
development and brain development•Helps infants gain weight faster•Recommended 300 mg/day for pregnant or lactating
women
•A lack of DHA is associated with Alzheimer’s disease•Inhibits the growth of human colon carcinoma cells•Increases the efficacy of chemotherapy
•Infant Health
•Adult Health
C22 H32 O2
INGREDIENTS: INTERESTERIFIED SOYBEAN OIL, WATER, ALGAE OIL, NATURAL SEA SALT, MONO/DIGLYCERIDES, NATURAL LECITHIN, SORBIC ACID, BETA-CAROTENE (VITAMIN A), ERGOCALCIFEROL (VITAMIN D), DIACETYL (FLAVORING)
DegummingBleaching
Removal FFADeodorization
CentrifugeCrude SBO
Citric Acid
GumWater
Citric Acid
Citric AcidAcid Activated Clay
Enzyme Acid ActivateClay
Interesterification
Hardfat SBO
Emulsification Pasteurization
Water
Sea Salt
Lecithin
Diacetyl
Mono/Diglycerides
B-Carotenes
Sorbic Acid
Ergocaliferols
Sodium methoxide
Citric Acid
Soft Water
Sodium methoxideCitric AcidSoft Water
ScrapedSurface HeatExchanger
Packaging
Refrigerant
To Wholesaler
DissolvedAir
FloatationFermentation Centrifuge
CO2
Lysing
Glucose
Ethanol StillBottom
Antifoam
Water
Algae
Glucanex
SteamCoolingWater
Sterile air
Vent
Water
Air CO2
Water
NaOH
Steam
Steam
Salt
CIPSystem
DryerAlgae Cake to
Wholesaler
Docosahexaenoic Acid
HO
O
Main Fermenter
Cooling Water
Sparged Steam
Vented Air13
Sterile Air
Sterile Glucanex Sterile
Anti-foam
7
8
11
Filter 1
Filter 2
P- 8
Filter 3
TIC
14
Compressor 1Air
PIC
PIC
FIC
QIC
QIC
9
P- 3
TIC
10QIC
Visc
Foam
QIC
P- 6
Turbidity
QIC
QIC
HX-1Fermentation Controls
35,000 gallon Fermentor
Height 50 feet
Diameter 12 feet
Total Project Capital Cost 37,328,232.77 USD
Operating Costs 18,962,032.85 USD/Year Raw Materials 6,031,280.00 USD/Year Utilities 3,532,157.83 USD/Year Maintenance 2,030,000.00 USD/Year Operating Labor Costs 2,828,000.00 USD/Year Operating Charges 707,000.00 USD/Year Plant Overhead 2,429,000.00 USD/Year G and A Costs 1,404,594.96 USD/Year
Total Product Sales 25,963,238.43 USD/Year
Payback Period 5.7 Years
Net Present Value 84,727,200.17 USD
Internal Rate or Return 25.5%
-$50
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Cumulative Cash FlowBasis: 1% of Market
Year of Plant Life
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Cumulative Cash FlowBasis: 2% in 6 Years
Year of Plant Life
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0 5 10 15
Millio
ns
Cum
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Cas
hflo
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Cumulative Cash FlowBasis: 2% of Market
Year of Plant Life
Expand to 2% Market Share in 6 Years
Payback Period 7.13 Years
Net Present Value 158,394,244.01 USD
Internal Rate or Return 34.5%
2% Market Share from Start
Payback Period 5.00 Years
Net Present Value 198,627,853.09 USD
Internal Rate or Return 34.5%
Growth Phase
Maintenance
What is Sanitary Design?Preventing microbial growth and simply being clean
How do you go about this?Physical separations of the different plantsAll equipment composed of stainless steelMargarine Plant composed of polished stainless steelLinear and horseshoe shaped plant designClean in Place system (CIP) on the insideSpray cleaning foam on the outside
Who cares?You do, and so do we. We do not want you getting sick from our product.
Algae Oil
Alpha Algae Co.1928 SE Otis Rd.Cedar Rapids, IA 52401
Alpha Algae Co.
Alpha Algae Co.
Truck Engines
Just Spread It On!
Sooo Healthy!0g Trans Fatty Acids100% Vegan100% Daily Omega-3 Fatty Acids per Serving
Eat Premium Golden rather than me for your essential fatty acids. Butter your biscuits!
We like it clean!
You should get DHA from algae just like me. It is tons healthier for both of us.
Algae Growth Cycle
0
5
10
15
20
25
30
0 20 40 60 80 100
Time (Hours)
Ce
ll D
en
sit
y (
g/L
)