2015 LanzaTech. All rights reserved.

Recycling Carbon with

Synthetic Biology

2016 LanzaTech. All rights reserved.

The LanzaTech process is driving innovation

Gas Feed Stream

Gas Reception Compression Fermentation Recovery ProductTank

• Process recycles waste carbon into fuels and chemicals

• Process brings underutilized carbon into the fuel pool via industrial symbiosis

• Potential to make material impact on the future energy pool (>100s of billions of gallons per year)

Novel gas fermentation

technology captures CO-rich

gases and converts the carbon

to fuels and chemicalsProprietary

Microbe

Gases are the soleenergy and carbon source

2

3

Staying within 2 degrees

65% of 2°carbon budget: USED

1870-2011: 1900 GtCO2

Remaining: 1000 GtCO2

To stay within budget biofuels consumption must grow

~350 billion gpy>1500 new 200M gallon

biofuel facilities

Must stay in the ground

Steel Gases: 30Bn Gal Ethanol Capacity

USA

925

BRAZIL

955INDIA

1,315

CHINA

10,800

RUSSIA

1,830

W. EUROPE

4,870

JAPAN

3,750

Steel Mills (>5 MT/year)

Country

Potential Ethanol Production Capacity (MMGPY)

Brazil

Argentina

Mexico

UnitedStates

Russia

Kazakhistan

Iceland

Australia

Thailand

Indonesia

China

S. KOREA

1,270E. EUROPE

1,300

TOTAL

27,015 MMGPY

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Gas Feed Stream

Gas reception CompressionFermentation Recovery Producttank

The LanzaTech Process

CO

CO2

5.2 barrels of gasoline are displaced by every

tonne of ethanol produced

1 tonne ethanol produced as CO averted from flare

Per tonne of LanzaTech Ethanol

CO2 MT kg PM kg NOx

Averted from flare 2.1 0.6 4.1

Displaced gasoline +0.5 +2.5 +7.4

Energy required for

LanzaTech Process-0.8 -0.2 -0.8

Avoided per

tonne of ethanol1.8 2.9 10.7

Broader Environmental Impact

LanzaTech Process emits

~40%

less NOx and ~80% fewer

particulates than electricity

generation per MJ energy

recovered

BiogasLFG, Methane

BiomassSolid WasteIndustrial, MSW, DSW

Waste carbon streams as a Resource

CO2

CO CO + H2 CO + H2 + CO2 CO2 + H2 CO2 + H2O + e-

Gas Fermentation

Reforming Gasification Renewable

ElectricityRenewable H2

Industrial Waste Gas

Steel, PVC, Ferroalloys

Available Most Point SourcedHigh Volume/Low Intrinsic Value

Non-Food

*2010 global production; 2012 proven gas reserves data (IEA, UNEP, IndexMundi, US DOE Billion Ton Update)

~ 1.4B MTA (Steel only) * ~184.2T M3 * >1.3B MTA (US Alone) *>2B MTA *

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LanzaTech’s Integrated Gas Fermentation Platform

Industrial provenhost strain

‒ Evolved proprietary strain with optimal performance, tolerance and robustness

‒ Chassis strains for key metabolic nodes

‒ Automatedevolutionplatform

Efficient genetic toolbox

‒ Robust tools (stable modular plasmids, genome engineering)

‒ Extensive Genetic parts library for precise control

‒ Automated DNA assembly platform

Integrated models and algorithms

‒ Codon usage algorithm and BioCAD

‒ Validated genome-scale model

‒ Process models and automation

‒ LCA and TEA

Process optimization and scale up

‒ Pure continuousprocess with high stability

‒ No sterility requirements (CO toxic and sole carbon)

‒ Proprietary scalable reactor designs

‒ Proven at scale at industrial sites

Feedstockflexibility

Low Cost

CarbonCaptureUtilization(CCU)

Products

Plug-in of existing pathways (in silico, in vitro; E. coli/yeast, retrosynthesis)

IP (>300 granted patents on all aspects of process)

Existing plants/ partners

8

1 Organism, over 20 Products…

Pyruvate

CO/H2

Acetyl-CoA

Fatty Acids,

Terpenoids

Aromatics

Ethanol

Succinate

Lactate

2,3-Butanediol

(2,3-BDO)

Biodiesel (FAEE)

3-Hydroxypropionate

(3-HP)

Isopropanol

Acetone

3-Hydroxybutyrate

(3-HB)

1,3-Butanediol (1,3-BDO)

Branched-chain

Amino Acids

Butyrate

1-Butanol

Methyl Ethyl Ketone

(MEK)

2-Butanol

Acetoin

1,2-Propanediol

1-Propanol

Jet Fuel

Isoprene

Aromatics

DiscoveryContinuous

lab-scale

fermentation

Scale

Up1 Organism, over 25 Products…

Acetate

Plug-and-play with engineered strains

Ethanol, 2,3-butanediol

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Same reactor

Same operating conditions

Same feedstock

“hardware”

“software”

improved efficiency, tolerance, ethanol only

new product molecule

Microbe 1.0

Microbe 1.1

Microbe 2.0

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Controlling Selectivity by Metabolic Engineering/ Process Conditions

Shift of flux between Acetyl-CoA derived products (Ethanol) and Pyruvate derived

products (BDO)

BDO pathway Knock-Out Unmodified BDO Process Optimization

Runs under controlled conditions for comparison,

flux can be pushed further to Ethanol and BDO by process control

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Metabolic engineering: Isopropanol and Acetone

Acetoacetyl-CoA

Thiolase

Acetoacetate

Acetyl-CoA

CoA transferase

Clostridial ABE pathway

Biomass Syngas

CO/CO2/H2

Acetyl-CoA

2x

AcetateATP

• Acetate as by-product• No net ATP generation

Acetone

C. autoethangenum native pathway

heterologous Clostridial ABE pathway

Acetoacetate decarboxylase

CO2 NADPH NADP+ i-propanol

Primary secondary alcohol

dehydrogenase

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LanzaTech Microbe 2.0: Isopropanol production

Thiolase

CoA transferase

decarboxylase

Secondary ADH

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Acetogens hold their own against established model organisms

Inokuma et al., 2010, Improvement of isopropanol production by metabolically

engineered Escherichia coli using gas stripping. J Biosci Bioeng, 110: 696-701.

PathwayNative producer

C. acetobutylicum/C. beijerinckii

Genotype -

Expression system

--

Enzymes

Nativenativenative

nativeSet Up Fed-batchSubstrate GlucoseMedia Complex

Production Rate<0.1 g/L/h

acetone/IPAOperational 3 days

May et al., 2012A modified pathway for the production of acetone in

Escherichia coli. Met. Eng,, 15:218-25.

Using a similar approach, same or higher rates and yields achieved from gas compared to sugar:

Best engineered strains from literature

E. coli E. coli

non-optimized strain non-optimized strainplasmid-based plasmid-based

inducible promoter inducible promoterThlA (Cac) ThlA (Cac)

YbgC (Hin)/CtfAB (Cac)AtoAD (Eco)/CtfAB

(Cac)Adc (Cac) Adc (Cac)

- SecAdh (Cbe)Fed-batch Fed-batchGlucose GlucoseComplex Complex

0.15 g/L/h acetone 0.67 g/L/h IPA

2 days 10 days

Acetogen

C. autoethanogenum

non-optimized strainplasmid-based

inducible promoterThlA (Cac)

CtfAB (Cac)Adc (Cac)

Native/KOContinuous

Steel Mill GasMinimal

>2.3 g/L/h acetone/IPA

>3 weeks

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Questions?

Gas Feed Stream

Gas Reception Compression Fermentation Recovery ProductTank