recombinant dna - western oregon universityguralnl/441recombinant dna.pdfdna ligase inks the strands...
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![Page 1: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/1.jpg)
Recombinant DNA• Restriction enzymes
& palindromes • Cloning with
plasmid vectors• Restriction mapping
& RFLPs• Probes• PCR analysis &
technique • DNA sequencing
![Page 2: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/2.jpg)
Restriction enzymes & palindromes
![Page 3: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/3.jpg)
Cloning with plasmid vectors
![Page 4: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/4.jpg)
Cloning
![Page 5: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/5.jpg)
Cloning
![Page 6: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/6.jpg)
Restriction mapping & RFLPs
![Page 7: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/7.jpg)
Probes
![Page 8: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/8.jpg)
Detection using probes
ASO= allele specific oligonucleotidesCan detect single nucleotide change
Sickle cell anemiaCystic Fibrosis
![Page 9: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/9.jpg)
PCR analysis & technique
![Page 10: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/10.jpg)
PCR applications
![Page 11: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/11.jpg)
DNA sequencing
![Page 12: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/12.jpg)
DNA sequencing
![Page 13: Recombinant DNA - Western Oregon Universityguralnl/441Recombinant DNA.pdfDNA ligase Inks the strands together FIGURE 12.7 Recombinant DNA molecules can be created using DNA from two](https://reader034.vdocuments.mx/reader034/viewer/2022050214/5f6047216d55474ff76240f6/html5/thumbnails/13.jpg)
Transgenic plants
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Gene Therapy