recombinant dnaboth dna strands of recombinant plasmids els j. m. verhoeven, and ben p. h. peeters...
TRANSCRIPT
Methods in Enzymology
Volume 153
Recombinant DNA
Part D
Ray Wu
Lawrence Grossman
CONTRIBUTORS TO VOLUME 153 ix
PREFACE Xiii
NATHAN 0 . KAPLAN XV
VOLUMES IN SERIES XVii
Section I . Vectors for Cloning DN A
1. Production of Single-Stranded Plasmid DNA
JEFFREY VIEIRAAND JOACHIM MESSING
A "
2 . pKUN, Vectors for the Separate Production of Rout) N . H . KONINGS ,
Both DNA Strands of Recombinant Plasmids ELS J . M . VERHOEVEN ,AND BEN P . H . PEETERS
12
3 . Restriction Site Bank Vectors for Cloning in JOHN DAVISON ,
Gram-Negative Bacteria and Yeast
MICHEL HEUSTERSPREUTE ,
AND FRANcOISE BRUNEL
34
4. Plasmids for the Selection and Analysis of Pro- JURGEN BROSIUS AND
karyotic Promoters
JAMES R . LUPSKI
54.
5 . A X DNA Protocol Based on Purification of Phage CYNTHIA HELMS ,
on DEAE-Cellulose
JAMES E . DUTCHIK, AND
MAYNARD V . OLSON
69
6. Double cos Site Vectors : Simplified Cosmid
PAUL BATES
82Cloning
7. Improved in Vitro Packaging of ,A DNA
SUSAN M . ROSENBERG
95
8 . A Phage Vectors-EMBL Series
A . M . FRISCHAUF ,
N . MURRAY ,AND H . LEHRACH
103
9 . Plasmid and Phage Vectors for Gene Cloning and DAVID A. Hopwoon ,Analysis in Streptomyces
MERVYN J . BIBB ,
KEITH F . CHATER, AND
TOBIAS KIESER
11 6
10 . Cosmid Shuttle Vectors for Cloning and Analysis R . NAGARAJA RAO ,
of Streptomyces DNA
M . A . RICHARDSON ,
AND S . KUHSTOSS
166
11 . Host-Vector Systems for Gene Cloning in Cyano- C . J . KUHLEMEIER AN Dbacteria
G . A . VAN ARKEL
19 9
12 . Genetic Engineering of the Cyanobacterial Chro- SUSAN S . GOLDEN ,mosome
JUDY BRUSSLAN, AN DROBERT HASELKORN
21 5
13 . Conjugal Transfer of Plasmids to Cyanobacteria TERESA THIEL AN DC . PETER WOLK
23 2
14 . Site-Directed Chromosomal Rearrangements in RICHARD T . SUROSKYYeast
AND BIK-KWOON TYE
24 3
15 . Improved Vectors for Plant Transformation : Ex- S . G . ROGERS, H . J . KLEE ,
pression Cassette Vectors and New Selectable R . B . HORSCH, AN DMarkers
R. T . FRALEY
25 3
16. Vectors for Cloning in Plant Cells
R . DEBLAERE ,A . REYNAERTS, H . HÖFTE ,J.-P . HERNALSTEENS ,J. LEEMANS, AN DM . VAN MONTAGU
277
17 . Binary Ti Vectors for Plant Transformation and GYNHEUNG AN
292
Promoter Analysi s
18 . Detection of Monocot Transformation via Agro- PAUL J . J . HOOYKAAS AN Dbacterium tumefaciens
RoB A . SCHILPEROORT
305
19 . Direct Gene Transfer to Protoplasts of Dicotyle- RAYMOND D . SHILLIT odonous and Monocotyledonous Plants by a AND INGO POTRYKUS
31 3Number ofMethods, Including Electroporation
20 . Uptake of DNA and RNA into Cells Mediated by W . H . R . LANGRIDGE ,Electroporation
B . J . LI, AN DA . A . SZALAY
33 6
21. Electroporation of DNA and RNA into Plant Pro- MICHAEL FROMM ,
toplasts
JUDY CALLIS ,LOVERINE P . TAYLOR ,AND VIRGINIA WALBOT
35 1
22 . Cloning Vectors of Mitochondria) Origin for Eu- CHRISTINE LANG-HINRICH Skaryotes
AND ULF STAHL
36 6
Section II . Vectors for Expression of Cloned Gene s
23 . Short Homopeptide Leader Sequences Enhanced WING L . SUNG, FEI-L . YAO ,Production of Human Proinsulin in Escherichia AND SARAN A . NARANG
385call
24 . Expression of Bovine Growth Hormone Deriva- HANSEN M . HSIUNG AN D
tives in Escherichia coli and the Use of the De- WARREN C . MACKELLAR
390rivatives to Produce Natural Sequence Growt h
Hormone by Cathepsin C Cleavag e
25 . Expression of Eukaryotic Genes in Escherichia BRIGITTE E . SCHONER ,
coli with a Synthetic Two-Cistron System
RAMA M . BELAGAJE, AN D
RONALD G . SCHONER
40 1
26. Expression of Heterologous Unfused Protein in ERIK REMAUT ,
Escherichia coli
ANNE MARMENOUT ,
Guus SIMONS, AN D
WALTER FIERS
41 6
27. Directing Ribosomes to a Single mRNA Species : ANNA Hut ,
A Method to Study Ribosomal RNA Mutations PARKASH JHURANI, AN D
and Their Effects on Translation of a Single HERMAN A . DE BOER
43 2Messenger in Escherichia col i
28 . New Expression Vectors for Identifying and Test- KLAUS SCHNEIDER AN D
ing Signal Structures for Initiation and Termina- CHRISTOPH F . BECK
452tion of Transcription
29_ Synthesis and Sequence-Specific Proteolysis of KIYOSHI NAGAI AN D
Hybrid Proteins Produced in Escherichia coli HANS CHRISTIAN TH¢GERSEN 46 1
30 . Expression Plasmid Containing the X PL Promoter GREGORY MILMAN
48 2and c1857 Repressor
31 . Expression and Secretion of Foreign Proteins in Guy D . DUFFAUD ,
Escherichia coli
PAUL E . MARCH, AN D
MASAYORI INOUYE
49 2
32 . Engineering for Protein Secretion in Gram-Posi- SHING CHANG
50 7
tive Bacteria
33 . Expression and Secretion Vectors for Yeast
GRANT A . BITTER ,
KEVIN M . EGAN ,
RAYMOND A . KOSKI ,
MATTHEW O . JONES ,
STEVEN G . ELLIOTT, AND
JAMES C . GIFFIN
51 6
34 . Vaccinia Virus as an Expression Vector
ANTONIA PICCINI ,
MARION E . PERKUS ,
AND ENZO PAOLETTI
545
AUTHOR INDEX 565
SUBJECT INDEX 593