Replace multi-step RNA purification methods with the RealTime ready Cell Lysis Kit, and go directly from cells to cDNA synthesis for high-performance real-time qPCR.

• Increase convenience: Purify RNA using a simple one-step cell lysis procedure instead of cumbersome multi-step protocols.

•Speed time to results: Lyse samples in just 5 minutes at room temperature, unlike other lysis methods.

•Reduce handling steps: Save time by performing DNase treatment during the cDNA synthesis incubation.

• Increase experimental flexibility: Escape from the workflow limitations of other suppliers’ all-in-one kits. Instead, perform multiple one-step RT-qPCR or cDNA synthesis reactions from each lysis.

RealTime ready Cell Lysis Kit Fast One-Step Cell Lysis for RT-qPCR

For life science research only. Not for use in diagnostic procedures.

Figure 1. Time savings and increased convenience in two-step RT-qPCR analysis of cell cultures*. Choose the Accelerated Workflow using the RealTime ready Cell Lysis Kit to cut your time in half for gene expression analysis in comparison to the Conventional Workflow.

* Calculation of time for the preparation of 96 cell culture samples.

Simplify Your Gene Expression Workflow by Significantly Reducing Handling Steps

Con

veni

ence

30 60 90 120 150

Accelerated Workflow

Conventional Workflow

Time-to-Result (minutes)

RealTime ready Cell Lysis Transcriptor Universal cDNA Master with DNase Treatment Real-Time PCR

Column-Based RNA Isolation cDNA Synthesis Real-Time PCR

Published by Roche Molecular Systems, Inc.Pleasanton, California, USA

https://lifescience.roche.com

© 2014 Roche Molecular Systems, Inc.

For life science research only. Not for use in diagnostic procedures.NOTICE: This product may be subject to certain use restrictions. Before using this product please refer to the Online Technical Support page (http://technical-support.roche.com) and search under the product number or the product name, whether this product is subject to a license disclaimer containing use restrictions.

RealTime ready DNA Probes Master: Under license from Gilead Sciences Inc.

HIGH PURE, LIGHTCYCLER, FASTSTART, and REALTIME READY are trademarks of Roche. SYBR is a registered trademark of Molecular Probes, Inc. Exiqon and ProbeLibrary are registered trademarks of Exiqon A/S, Vedbaek, Denmark. All other product names and trademarks are the property of their respective holders.

Rapid Gene Expression Analysis — Directly from Cells!

Figure 2. Real-time PCR results using the Accelerated Workflow with the RealTime ready Cell Lysis Kit, and the Conventional Workflow using the column-based High Pure RNA Isolation Kit. Real-time PCR analysis shows accurate, reproducible results for both RNA isolation techniques, resulting in high correlation of quantification cycles (Cqs) for the 19 different reference genes tested.

Experimental setup: 30,000 HeLa cell equivalents/well were used for RNA preparation. RNA was reverse transcribed using the Transcriptor First Strand cDNA Synthesis Kit. Real-time PCR amplification was performed on a real-time PCR instrument (96-well format, 20 µl reaction), using the LightCycler® 480 Probes Master and RealTime ready Human Reference Gene Panel.

RealTime ready Cell Lysis Kit (Cq)

Hig

h P

ure

RN

A Is

olat

ion

Kit

(Cq)

Cat. No. Product

06 366 821 001 05 943 523 001

RealTime ready Cell Lysis Kit50 x 40 µl reactions500 x 40 µl reactions

07 248 431 001

RealTime readyCell Lysis Buffer5000 x 40 ul reactions

03 335 399 001 03 335 402 001

Protector RNase Inhibitor2000 U5 x 2000 U

Cat. No. Product

04 379 012 001 04 896 866 001 04 897 030 001

Transcriptor First Strand cDNA Synthesis Kit 50 x 20 µl reactions 100 x 20 µl reactions 200 x 20 µl reactions

05 893 151 001

Transcriptor Universal cDNA Master 100 x 20 µl reactions

Streamline Your Gene Expression Workflow and Speed Time to Results

Cat. No. Products for use on the LightCycler® 480 System

04 707 494 001 04 887 301 001

LightCycler® 480 Probes Master 5 x 1 ml (500 x 20 µl reactions) 10 x 5 ml (5,000 x 20 µl reactions)

04 707 516 001 04 887 352 001

LightCycler® 480 SYBR Green I Master 5 x 1 ml (500 x 20 µl reactions) 10 x 5 ml (5,000 x 20 µl reactions)

05 502 381 001 RealTime ready DNA Probes Master (1,250 x 20 µl reactions)

RealTime ready assays

Cat. No. Products for use on other qPCR instruments

04 913 850 00104 913 914 001

FastStart Universal SYBR Green Master (Rox)4 x 1.25 ml for 500 reactions of 20 µl10 x 5 ml for 5,000 reactions of 20 µl

04 913 949 00104 913 957 001 04 914 058 001

FastStart Universal Probe Master (Rox) 2 x 1.25 ml for 250 reactions of 20 µl 10 x 1.25 ml for 1,250 reactions of 20 µl 10 x 5 ml for 5,000 reactions of 20 µl

Universal ProbeLibrary assays

Cell Lysis cDNA Synthesis DNase Treatment

Real-Time PCR

qPCR results in just 75 minutes...

Cell Lysis

Visit www.gene-expression.roche.com to learn more about these and other Roche products for fast, accurate gene expression analysis.