rare donor program in japan

Japanese Red Cross Kinki Block Blood Center

Rare donor program in Japan

Yoshihiko Tani, MD, PhD


ISBT Working Party on Rare Donors


Have a national Rare Donor Program


Rare Blood in Japan

Category I (35)

Category II (5)

Bombay para-Bombay Mk/Mk En(a–) MiV/MiV

Nsat/Nsat S–/s–/U– p Pk Rhnull Rhmod

–D– cD– LW(a–b–) Lu(a–b–) In(Lu)

Ko Kp(a+b–) Kp(a–b–) K14 – k– Kmod McLeod

Fy(a–b–) Jk(a–b–) I – Ge:–2,–3 Lan– IFC–

UMC– Dr(a–) Gy(a–) Ok(a–) JMH– Er(a–)

s– Fy(a–b+) Di(a+b–) Jr(a–) Do(a+b–)


Number of Donors in Database


Registration of Rare Donors in Japan (Category I)

Rare Blood RhD + RhD –

Bombay 5 0

MkMk 1 0

En(a–) 2 0

MiV / MiV 4 0

Nsat/Nsat 1 0

S–s–U– 2 1

Pk 2 0

Rhnull － 5

Rhmod － 7

Kp(a–b–) 2 0

k– 8 0

K:–14 2 0

Fy(a–b–) 6 0

as of 2012.3.31


McLeod 19 0

I – 19 0

Ge– 11 0

Lan– 16 0

Gy(a–) 6 0

Ok(a–) 19 0

JMH– 12 0

para- Bombay 77 0

p 25 1

D– – 103 －

In(Lu) 93 1

Ko 100 2

Jk(a–b–) 69 1


Registration of Rare Donors in Japan （Category Ⅱ）


Fy(a–) 3,117 495

Di(b–) 2,671 101

Jr(a–) 2,130 42

s– 342 106

Category I

as of 2012.3.31


Number of Rare Blood Donors registered (Category I) (1996 - 2011)


Number of Rare Blood Donors registered (Category II) (2000 - 2011)


Number of Frozen Rare Cells

Number of Frozen Rare Cells

Rare Blood A O B AB

+ – + – + – + –

Bombay 18

para-Bombay 79 60 16 4

Rhnull 13

Rhmod 9

D– – 89 25 23 20

p 1 3 6 4

Pk 3

En(a–) 8 15

MiV/MiV 7

Ko 50 55 2 51 1 32

McLeod 18 14 32 4

Jk(a–b–) 53 64 4 26 29

I– 15 10 5 10

Ge– 24 11 13

Lan– 19 20 8 3

Gy(a–), Hy– 6 1

Ok(a–) 17 9 6 18

Fy(a–b–) 14 11 3 1 1 1

In(Lu) 37 4 35 33 7 31

numbers

as of 2012.3.31


How are Rare Donors found?


Monoclonal antibodies for blood grouping (1)

Cell line MoAb Cell line MoAb Cell line MoAb

OSK1 anti-CD44

OSK7 anti-Pk OSK15-1 anti-P

OSK1-1 OSK8 anti-U＋N OSK15-2

OSK2 anti-Rh29 (anti-Rh17)

OSK9 anti-A

OSK16 anti-H

OSK2-1 OSK9-1 OSK16-1

OSK2-2 OSK10 anti-B

OSK17

anti-P1 OSK3

anti-D

OSK10-1 OSK17-1

OSK3-1 OSK10-2 OSK17-2

OSK3-2 OSK11 anti-M OSK17-3

OSK3-3 OSK12 anti-N

OSK18 anti-Le related

OSK3-4 OSK12-1 OSK18-1 anti-LebH

OSK4 anti-EnaFR OSK13 anti-Lea

OSK19 anti-Fy6

OSK4-1 anti-EnaFS OSK13-1 OSK20

anti-E OSK4-2 anti-EnaTS OSK13-2 OSK20-1

OSK5 anti-K2 OSK14 anti-I OSK20-2

OSK6 anti-Ge OSK15 anti-P OSK21 anti-Acquired B

red：Human MAb ：rare blood screening


Monoclonal antibodies for blood grouping (2) Cell line MoAb Cell line MoAb Cell line MoAb

OSK22

anti-Fy3

OSK29-2

anti-

GPA&B

OSK36 anti-Kpb

OSK22-1 OSK29-3 OSK37 anti-Dia

OSK22-2 OSK29-4 OSK38 anti-Xga

OSK23 anti-T OSK29-5 OSK39 anti-IFC

OSK24 anti-Tn

OSK29-6 OSK40 anti-Miltenberger

OSK24-1 OSK29-7 OSK41 anti-Fyb

OSK25 anti-K14

OSK29-8 anti-GPA OSK42 anti-e

OSK25-1 OSK30 anti-Jra OSK43 anti-Lan

OSK26 anti-c OSK31 anti-Dib OSK44 anti-Hro

OSK27 anti-Tk OSK32 anti-K5

(Ku)

OSK46 anti-Kg

OSK28 anti-i OSK33 anti-Kpc OSK47 anti-Fya

OSK29 anti-GPA&B

OSK34 anti-K1 OSK48 anti-C

OSK29-1 OSK35 anti-Jkb OSK49 anti-S

OSK-MoAb: 49 kinds, 93 cell lines red：Human MAb ：rare blood screening


Blood Grouping Reagent :

Cross-linked Monoclonal antibody

Mouse anti-human IgG MoAb

mix

Human MoAb

Red Blood cells

Cross-linked MoAb agglutination


Total volume of monoclonal antibodies supplied to other blood centers（1987-2009）

Year No. of

MoAbs No. of Blood

centers supplied Total volume

(mL)

1987

1988

1989

1990

1991

1992

1993

1994

1995

1996

1997

1998

1999

2000

2001

2002

2003

2004

2005

2006

2007

2008

2009

2010

2011

6

8

14

15

20

24

24

27

28

30

26

31

27

36

31

34

32

30

37

34

33

35

28

32

39

19

23

19

21

23

23

22

30

45

41

31

36

25

28

32

34

34

37

34

28

19

12

10

10

10

21,890

18,290

15,540

16,840

19,845

20,460

18,045

30,348

50,301

59,018

49,187

32,217

28,655

39,731

56,962

75,770

77,005

87,590

111,910

97,630

125,050

255,365

330,512

397,204

455,610

1987~： anti-Ena, anti-Hro(Rh17)

anti-K2, anti-Ge

anti-U+N, anti-CD44

1991~： anti- I

1994~： anti-Jra, anti-Dib

2005~： anti-Lan

2007~: Consolidating of laboratory

・Screening of rare blood

・Screening of antigen negative blood

・Blood typing

Japanese Red Cross OSAKA Blood Center

Automated machine

PK7300


Images of hemagglutination on PK7300

D ABO antibody rare blood

1

2

3

4

5

6

7

8

9

10

A D − Ab−

A D＋ Ab−

A D＋ Ab−

O D＋ Ab−

O D＋ Ab−

O D＋ Ab−

B D＋ Ab−

B D＋ Ab−

AB D＋ Ab−

AB D＋ Ab−

detamination


Number of Rare Donor units used per year


Units of Rare Blood Provided (Category I) (1997 – 2011)


Units of Rare Blood Provided (Category I)

(2000 – 2011) units

0

10

20

30

40

50

60

D– – Jk(a–b–) p Ko Bombay(para)

2011


Units of Rare Blood Provided (Category II)

(2000 - 2011)

0

200

400

600

800

1.000

Jr(a–) Fy(a–) Di(b–)

Units

2011


Units of Rare Blood Internationally shipped

(1977–1998) year country rare blood units

1977 Germany(W) Di(b–) 6

1981 Germany(W) Di(b–) 16

1982 USA

Germany(W)

D– –

Di(b–)

19

9

1983

Germany(W)

Germany(W)

France

Di(b–), Ccee, Fy(b-)

Di(b–), Jk(b-)

D– –

6

13

12

1984 Thailand

Germany(W)

Bombay(Para-Bombay)

Di(b–), Jk(b-)

5

16

1985 Thailand

Germany(W)

p

Di(b–), Jk(b-)

4

10

1986 Germany(W)

Di(b–), Jk(b-)

Di(b–)

Ko

20

9

6

1987 Germany(W) Ko 6

year country rare blood units

1988

USA

Germany(W)

Thailand

MkMk

Ko

Bombay

6

8

4

1989

USA

Germany(W)

Thailand

Jr(a–)

Ko

Di(b–)

4

4

14

1991 Thailand Bombay 7

1995 Australia

Thailand

Di(b–)

Ko

8

9

1996 Sweden Di(b–) 8

1997 Taiwan

USA

Jk(a–b–)

D– –

11

6

1998

Switzerland

Norway

Argentina

UK

Ko

Jr(a–)

D– –

Jr(a–)

8

8

4

4


Units of Rare Blood Internationally shipped

(1999–2012.3) year country rare blood units

1999

Sweden

USA

Netherlands

En(a–)

Jr(a–)

Ko

12

4

10

2000 Indonesia

UK

Para-Bombay

D– –

10

6

2001 China

Canada

Di(b–)

D– –

32

2

2002 Philippine Jk(a–b–) 2

2003 Spain D– – 8

2004

Philippine

USA

Korea

Brazil

Brazil

Jk(a—b–)

Jr(a–)

D– –

D– –

Ko, D–

12

40

8

6

4

2005

Thailand

Czech Republic

Philippine

p

D– –

Jk(a–b–)

10

8

15

year country rare blood units

2006 Thailand

China

Ko

Fy(a–), C–

8

16

2007 New Zealand D– – 6

2008 Netherlands

Thailand

Di(b–), D–

Fy(a-),C-,e-,Jk(b-)

4

12

2009

Netherlands

Thailand

Malaysia

Di(b–), D–

Fy(a-),C-,e-,Jk(b-)

p, Fy(b–)

4

12

2

2010 UK

Australia

D– –

p

4

4

2011

Canada

UK

New Zealand

Ko

D– –

D– –

11

8

4

2012 Australia Lan– 4


Units of Rare Blood Internationally shipped–1

(1977–2012.3) country year rare blood units

Germany

(West)

1977

1981

1982

1983

1984

1985

1986

1987

1988

1989

Di(b–)

Di(b–)

Di(b–)

Di(b–), Fy(b-),Ccee

Di(b–), Jk(b-)

Di(b–), Jk(b-)

Di(b–), Jk(b-)

Di(b–), Jk(b-)

Di(b–)

Ko

Ko

Ko

Ko

6

16

9

6

13

16

10

20

9

6

6

8

4

France 1983 D– – 12

Norway 1998 Jr(a–) 8

Sweden 1996

1999

Di(b–), C–, S–, P2

En(a–)

8

12

country year rare blood units

Netherlands 1999

2009

Ko

Di(b–), D–

10

4

Switzerland 1998 Ko, c–E–,Fy(b-) 6

UK

1998

2000

2010

2011

Jr(a–)

D– –

D– –

D– –

4

6

4

8

Spain 2003 D– – 8

Czech Republic 2005 D– – 8

USA

1982

1988

2004

2005

D– –

MkMk

Jr(a–)

Jr(a–)

Jr(a–)

19

6

4

40

16

Canada 2001

2011

D– –, Fy(b–), Jk(a–)

Ko

2

11


Units of Rare Blood Internationally shipped–2

(1977–2012.3)


Taiwan 1997 Jk(a–b–) 11

Hong kong 1997 Di(b–) 8

China 2001

2006

Di(b–), Jk(a–)

Fy(a–), C–

32

16

Korea 2004 D– – 8

Australia

1995

2010

2012

Di(b–)

p

Lan–

8

4

4

New Zealand 2007

2011

D– –

D– –

6

4

Argentina 1998 D– – 4

Brazil 2004 Ko, D–

D– –

4

6


Thailand

1984

1985

1988

1989

1991

1995

2005

2006

2009

Bombay(Para-Bombay)

p

Bombay

Di(b–)

Bombay

Ko

Jk(a–b–)

p

p

Ko, Le(a–)

Fy(a–), C–e–, Jk(b–)

5

4

4

14

7

9

9

4

6

8

12

Philippine 2004

2005

Jk(a–b–), c–E–

Jk(a–b–)

12

15

Indonesia 2000 Para-Bombay 10

Malaysia 2009 p, Fy(b–) 4


Countries & Rare Blood Internationally shipped

(1977-2012.3)

Germany, Sweden, Norway, France, Netherlands, UK,

Switzerland, Spain, Czech Republic,

USA, Canada, Argentina, Brazil,

Thailand, Philippine, China, Taiwan, Hong Kong,

Korea, Indonesia, Malaysia, Australia, New Zealand

23 countries

rare blood units numbers rare blood units numbers

Di(b–) 179 25 Bombay (para-) 26 7

D– – 95 17 p 22 5

Jr(a-) 72 6 En(a–) 12 1

Ko 72 15 MkMk 6 3

Jk(a–b–) 47 10 Lan– 4 2

Fy(a–) 28 4 Total 563 92


Screening for rare blood by monoclonal antibodies (1987 ~ 2011)

Monoclonal Antibody No. of tested Rare Blood No. of detected Frequemcy

anti-U+N 2,062,873 U– 3 0.00015 %

anti-Ena 12,842.271 En(a–) 2 0.00002 %

MkMk 2 0.00002 %

anti-Hro 25,703,046 D– – 217 0.00084 %

Rhnull 10 0.00004 %

anti-CD44 6,850,028 Lu(a–b–) 622 0.00908 %

anti-K2, anti-K5,

anti-K14 17,265,220

Ko 306 0.00177 %

McLeod or Kmod 186 0.00108 %

anti-Ge 7,828,135 Ge– 20 0.00026 %

anti-Lan 7,197,279 Lan– 69 0.00096 %

anti-Fy3 795,239 Fy(a–b–) 0 0 %

anti-IFC 610,271 IFC– 1 0.00016 %

anti-I 1,138,211 I– 0 0 %

anti-Jra 21,161,014 Jr(a–) 11,026 0.05211 %

anti-Dib 2,532,761 Di(a+b–) 4,763 0.18806 %

Including repeat donors


Frequency of Rare Blood (Japan)

1/100

1/1,000

1/10,000

1/100,000

1/1,000,000

Fy(a–)

Di(b–), s–

Jr(a–)

Lu(a–b–), InLu Ko, Lan– Jk(a–b–), Ok(a–) Ge–

–D–

Rhnull

I– Oh P Fy(a–b–) En(a–) EnaFR– (MiV/MiV, NSAT/NSAT) IFC– Dr(a–)

Gy(a–), Kx–(McLeod)

Summarized by Dr. Uchikawa in Tokyo BC


Other items of interest


Rare Blood Internationally Provided

to Japan

rare blood country units

1974 –D– Canada 4

1977 p USA

Canada

2

2

1984 I – USA 2

1985 En(a–) UK 2

1999 Ge – Sweden

USA

4

4

Thank you for your attention.

38


rare donor program in japan

Documents