RAPID DNA:PILOT STUDY FOR LAW ENFORCEMENT

Michael P. Garvey, Jr, PhD

Director, Office of Forensic Science

Philadelphia Police Department

Stephanie Stoiloff

Senior Police Commander, Forensic Services Bureau

Miami-Dade Police Department

Jan Girten

Director, Forensic Services

Colorado Bureau of Investigation

Rapid DNA Automated DNA process Fieldable platform Non-expert user 90-120 minute swab to answer Reference or Evidence

VS

Is it us against them?

We need to start somewhere…

Law Enforcement Organizations

Philadelphia Police

Colorado Bureau of Investigation

Miami Dade Police

Rapid DNA Vendors

GE DNAscan™ Rapid DNA Analysis™ System

IntegenX® RapidHIT® Human DNA Identification System

Validation & Pilot Studies

Internal Validation (100 items)

Sensitivity Repeatability Reproducibility Controls Precision Mixture Mock Evidence

Reference Limited Forensic

Operational Pilots Reference samples

Lab based Field based General purpose vs. targeted

collections Arrestee collections

Limited forensic evidence Confirmatory testing Sample size Intelligence value

Goals Law Enforcement and Forensic evaluation of current

state of technologies through two independent assessments involving multiple labs

Data shared with LE and Forensic communities to allow informed decisions

Cost comparisons Operational scenarios Development of LE and forensic protocols Work with other labs who are participating in efforts

to complete testing for NDIS approvals, etc.

Current Status Philadelphia Police Department

DNAscan (GE Healthcare) Complete: delivered, installed, training, internal validation Pending: evaluation of forensic cartridges and operational pilot program

RapidHIT (IntegenX) Complete: delivered, installed, training In progress: internal validation of reference and forensic samples Pending: Operational pilot program

Miami Metro Dade Police Pending delivery and installation

Colorado Bureau of Investigation Pending delivery and installation

The purpose of these studies is to provide an evaluation of each of the leading vendors of Rapid DNA.

Each study/pilot is an independent assessment. It has not been designed or performed as a head to head comparison. Any comparisons will be left to the informed consumer.

Philadelphia Office of Forensic Science DNAscan Evaluation

April 2015

Internal validation summary of samples tested

Promega PowerPlex™ 19 runs, 95 samples from 20 known donors

60 single source samples Sensitivity Reproducibility Repeatability NIST traceable

25 mixture samples 10 blanks

DNAscan™ :References DNAscan RFID swab used for all samples 60 reference buccal swabs from 20 known donors

55 generated a full 16 locus profile without human intervention. 4 generated a 16 locus profile with minimal human intervention

Human intervention was limited to exporting data then importing into GeneMapper® ID-X v1.2 software for analysis.

1 sample generated partial profile at (1 swipe) Mixtures

1:1 -- all flagged as mixtures 5:1 -- 1 sample in each mixture set flagged as mixture, others only major 19:1 – all called as major contributor only

Controls All as expected

6 swipes

3 Swipes

1 swipe

DNAscan™ : Forensic Pending

Evaluation of the forensic (low concentration) cartridge

Philadelphia Office of Forensic Science RapidHIT Evaluation

April 2015

Test Plan and Success RatesSample Type DescriptionRapidHit # of Samples

RapidHIT % loci generated

Positive Control NIST traceable swabs 2 100

Negative Control Blank wells 7 0

Sensitivity Cotton swabs - 10, 5, 2, 1 swipes; 1 touch 10 99.1

Precision  Allelic ladders 17 ±0.05

bp std. dev.  Accuracy and Repeatibility Bode Buccal Collector punches (6 mm) 6 97.1

  Cotton Swabs 9 99.5

  Blood punches (6 mm) 4 70

  Blood on swab (5 ul) 2 100

Mixtures19:1, 9:1, 5:1, 1:1, 1:5, 1:9, 1:19  (total vol 5 ul blood) 7 NA

Semen Neat semen on swab (1 ul) 1 100

Cigarette Butt Cutting 1 72

Water Bottle Cotton Swab 1 95.8

Blood on Denim Cutting on Denim (5 ul) 1 100

Preliminary Data: Validation still in progress

5uL Blood on Denim (direct insertion)

1uL Semen on Swab

Cigarette Butt Filter Paper (direct insertion)

Plastic Water Bottle (swabbed)

Future Complete the studies Present to LE and Forensics Develop recommendations, training, and

protocols Cost analysis Technical analysis Operational analysis