RABIES VIRUSRABIES VIRUS

What’s this?What’s this?

• Morphology and structure• Replication• Pathogenesis • Clinical symptoms• Diagnosis• Prevention and Treatment

Morphology Morphology • bullet-shaped; • 45-100 nm in diameter; 100-430 nm long.

structurestructure• Core -ssRNA• L-protein (large protein)• ---RNA dependent RNA polymerase RNP Capsid N-protein ---protect RNA from hydrolysis P-protein

Envelope Bilipid layer• M-protein (matrix protein)• G-protein (glucoprotein)• ---spike• ---bind to the receptor of the host cell•

• Rabies is an RNA virus. The genome encodes 5 proteins designated as N, P, M, G, and L. The order and relative size of the genes in the genome are shown in the figure below. The arrangement of these proteins and the RNA genome determine the structure of the rabies virus.

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ReplicationReplication• Adsorption• Penetration• Uncoating• Biosynthesis• Assembly and release

biosynthesisbiosynthesis• -ssRNA• RNA polymerase

• +ssRNA

• -ssRNA protein• • virus

PathogenesisPathogenesis• CNS

afferent fibers efferent fibers nerve endings salivary glandNeuromuscular organs and tissuesjunction muscles Virus

Clinical symptomsClinical symptoms• Early stage (1-3days)• fever(37.5-38)• vomit• wound:pain,red,itching• Exciting stage(1-3days)• fever(39-40)• hydrophobia:pharyngeal muscle cramp,dyspnea• dehydration• Paralytic stage(15-20hs)• nerve fiber paralysis• respiratory and circulatory failure death

DiagnosisDiagnosis

• Pathogenic history• Isolating the dog(7-10days)

cerebral smear or slice immunofluorescent stain

Prevention and treatmentPrevention and treatment

• Kill wild dogs• Keep away from

dogs

• Clean the wound• Passive immunity• vaccine

ContributersContributers• Bian Shu• Cong Ping• Mu Xueru• Shao Cong• Zhang Jingjing• Zhu Wenying

• Cheng Wei• Hu Chen• Li Yuntao• Li Daowei• Wang Shengxi