queen rearing: malaysia status · meliponiculture. introduction the queen bee of stingless bees are...
TRANSCRIPT
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MARDI
Queen rearing : Sustainable the Meliponiculture
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Introduction
The queen bee of stingless bees are unable to find new colonies independently.
They also do not exploit colonies of other species, as do some ants that practice dependent nest foundations.
Consequently the colonies must multiply by swarming- the queen and a group of workers depart from the mother colony in order to find a new one.
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To date there are more than 600 farmers involved in stingless bee rearing with almost 20,000 colonies in the country.
Demand for feral (wild) colonies of stingless bee are very high since the price of stingless bee honey catches a premium price.
Trees being cut down unselectively which in the end resulted to the destruction of the natural ecosystems
Therefore new and fast multiplication method for stingless bee queen rearing must be established in order to overcome some of the problems indicated before.
One of the fast techniques is rearing of the queen via in-vitro technique
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Important of queen rearing
Reduce price of the colony.
Sustainable the environment.
Maintaining the industry.
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Our focus
Produce virgin queen
Enhance meliponicuture with box style
If got 5 colonies from the project also the best result.
Mobile Meliponiculture (Mobile Pollination Services)
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Honey industrySABAH
Highest no of
farmers (95%)
No. of farmers
651 org
605 org
134(K) Kg
Sales (RM)
27(K) Kg
Production (Kg)
RM 2j
RM 20j
SARAWAKHighest no. of honey
production
(51%)
SARAWAKHighest no of sales
(42%)
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Our basic dataOvipositioning
behaviour
Foraging behaviour
Pollen types
Mating behaviour
Diversity and
abundance
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Stingless Bees Mapping
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Abundance of stingless bee species in Peninsular Malaysia from June 2013 to June 2015
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Observation colony
The colony placed in the lab to study the food source
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Total pollen collected from 8am to 3pm
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Pollen
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Ovipositioning process
Fastest oviposition
was 4 second
Ovipositioningprocess start from 7
am and happen once every two
hours
Brood cell built every 2 hours and takes 2 hours to
complete
Mean no. egg laid by queen per day was
45±
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Trend of brood produced by H.itama from Jun to December
y = 0.4286x + 47.857
R² = 0.2798
45.0
46.0
47.0
48.0
49.0
50.0
51.0
52.0
53.0
June July August September October November December
Nu
mb
er o
f b
ro
od
per d
ay
Sampling Month
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Queen rearing started
2014 First study
2015 failed2016 give
up when no attention
2017 grant from MARDI
2018 method
established
2019 farm test
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• 156 trial since 2014.
• Sacrifice about 312 layer of brood which is similar to 30 colonies.
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Failed again
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Failed again Worker
The eggs not turn into
larva
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Larval food test
No Larval food test Observation
1 60 µl The eggs transform to larva stage in 3 days and become worker in 30± days.
2 80 µl The eggs transform to larva stage in 3 days and become worker in 45± days.
3 100 µl The eggs transform to larva stage in 3 days. 50% of sample change into queen in 75± days.
4 120 µl The eggs transform to larva stage in 3 days. 50% of sample change into queen in 85± days.
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Healthy larval
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Here the successful story
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Our improvement (come the headache)
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Here the queen
The queen from the plate
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Field test
6 inchi
6 inchi
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Challenge
Uneven hatching
Humidity
The eggs (which eggs u must choose)or it will turn into drone
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The technique
1. Collect the larval food, store at fridge.
2. Prepare 96 well Elisa Plate
3. Harvest the young brood cell (Egg)
4. Insert the liquid food into ELISA well
5. Insert the egg vertically
6. Close the ELISA Plate than put in incubator.
7. Control the humidity and wait
8. Good Luck
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Special thank to
KETENGAH for funding the basic study(KG0007410).
MARDI for funding preliminary study
MOA for mass scale (KG002910).
Farmers that I took the brood for samples