Slide 1

QuattroMix Single Use Mixing System For Single Use Bag Integration work In cooperation with:

Slide 2

QuattroMix Principles Application: Design 16 outlet nozzles Single Use Bag Theoretical fluid stream Connected with pump in- and outlet Exocirculation Via pump Random Vector No Vortexing

Slide 3

Performance test 500L Solid/liquid mixing performance: Addition of NaCl to 500L Water 4g/l Completely dissolved after 10 min 2g/l; Completely dissolved after 5 min 500L bag with two QuattroMixers, in parallel connected to one QF1200SU.

Slide 4

Applications Typical application in biopharmaceutical processes Buffer preparation Media preparation Virus inactivation And others Salts Purified Water Acids/ Bases Carbohydrates Nutrients SU Bag with Mixer Sterile Filtration Upstream or Downstream Process

Slide 5

QuattroMix Enables: Quattroflow Pump becomes a multi purpose device, because Recirculation of liquid is used for mixing effect Can be used for filling and emptying of the bag Can be used for post-mixing processes, like sterile filtration, virus filtration, flow through chromatography, etc. Recirculation loop allows for additional features like Integration of in-line sensors (pH, conductivity, temperature, UV) Usage of heat exchanger for cooling/heating of media Filter Filtrated Product Heat exchanger TIR Feedstock

Slide 6

JetMixer Solid/liquid mixing performance: Addition of 500 g NaCl to 200L Water Result: Better performance of QF mixer Still undissolved NaCl after 18 minCompletely dissolved after 4 min Comparison 200L

Slide 7

Performance test JetMixer Liquid/liquid mixing performance: adding 100 g predesolved NaCl to 200L Water Result: Comparable mixing performance:

Slide 8

Comparison 200L JetMixer QuattroMix

Slide 9

Available For: Application development work Mixing problem resolution Bag Sizes: 100L, 200L with single mixer 500L with dual mixer Bag Configuration/porting On request to HPNE From: HPNE