B.S (Hons.) 3rd Semester Organic Chemistry Practicals

BS (Hons) 2nd Semester Organic Chemistry Practical Manual

ExperimentTitle:Qualitative tests for fats and oils.

ExperimentsObservationsInferences

Solubility Test

(i) Org. compound + H2O

(ii) Org. compd. + chloroform or etherInsoluble

SolubleFats and oils present

Fats and oils present

LitmusTest

Org. compd. + litmus sol. No change in colourFats and oils present

Acrolein Test (For glycerol)

Organic compd. + KHSO4 + heatIrritating smell of acrolein (CH2=CH-CHO)Fats and oils present

Unsaturation Test

1 mL of alcoholic solution of organic compound + 1-2 drops of bromine water ( or dil. KMnO4)Colour of bromine is dischargedUnsaturated fats and oils present

Soap formation (Sponification test)

Organic compound + Alcoholic solution of NaOH + heatSoap is formedFats and oils present

Liebermann-Burchards Test(For Cholesterol)

3 ml of org. compd. (Cholesterol soln) + 10 drops of acetic anhydride + 1-2 drops of conc. H2SO4 + mix.Red colour at first and followed by blue and finally becomes greenFats and oils present

1Dr. Muhammad Aslam

Assistant Professor-Chemistry

University of Education, Township Campus, Lahore.