FSC-A

SSC

-A

Live/Dead

SSC

-A

FSC-A

FSC

-H

SSC-A

SSC

-W

92.7%

CD11c

SSC

-A

CD11b

Gr1

52.7%

23.2%

4.57%

CD11c

MH

IIC

4.47%

CD3

NK

1.1

2.72%

5.29%

CD19

CD

34.71%

6.52%

P2X7

SSC

-A

Neu/mono

23.1%

P2X7

SSC

-A

DC

3.26%

P2X7

SSC

-A

NK

3.41%

P2X7

SSC

-A

T cells

CD45SS

C-A

3.85%

P2X7

SSC

-AB cells

Qin et al., Sup Fig. 1A

B

84.6%

F4/80

SSC

-A

83.2%

P2X7

SSC

-A

TAM

P2X7

+ CD

68+

cells

/fiel

d

P2X7CD68

DAPI MergeHuman

50μm

Qin et al., Sup Fig. 2A B

D

C

ID8 intraperitoneal injection

P2X7+/+

P2X7-/-

12.1% 6.15%

F4/80-APC

CD

206-

PE

P2X7+/+ P2X7-/-

Orthotopic

P2X7+/+

P2X7-/-

P2X7+/+

P2X7-/-

Ort

hoto

pic

CD206CD68DAPI Merge

Perc

ent s

urvi

val

Day

P2X7+/+P2X7-/-

P=0.0485

E

G

F

Mill

ilite

rs

Ascites volume

P2X7+/+ P2X7-/-

Perc

ent o

f M2

mac

roph

age

(%)

P2X7+/+ P2X7-/-

0

20

40

60

80

100 ***

P2X7+/+ P2X7-/-

CD

206+

/F4/

80+ (

%)

100μm

100μm

50μm

50μm

Qin et al., Sup Fig. 3

Medium LPS+IFNγ

F4/80

CD

16/3

2

16.7%

15.9%

44.7%

44.2%

P2X7+/+

P2X7-/-

B

D

CA

P2X7+/+P2X7-/-

Rel

ativ

e ex

pres

sion

of i

NO

S

Control LPS+IFNγ

BMDMP2X7+/+P2X7-/-

Rel

ativ

e ex

pres

sion

of T

NFα

Control LPS+IFNγ

BMDM

P2X7+/+P2X7-/-

0

20

40

60

80

100ns

Rel

ativ

e ex

pres

sion

of C

XCL1

0

Control LPS+IFNγ

BMDM

P2X7+/+P2X7-/-

Perc

ent o

f M1

mac

roph

ages

(%)

Control LPS+IFNγ

BMDM

Qin et al., Sup Fig. 4BA C

Rel

ativ

e ex

pres

sion

of F

izzl

ControlLLC-CMLLC-CM+ O-ATP

E

D

F G

K L M

H

Rel

ativ

e ex

pres

sion

of A

rg1

MH-S

Rel

ativ

e ex

pres

sion

of F

izzl

MH-S

MFI

Nor

mal

ized

to M

ode

CD206

MH-S

Control IL4 IL4+ O-ATPIL4+ A-43 IL4+ A-74

Rel

ativ

e ex

pres

sion

of A

rg1

ControlIL4IL4+ O-ATP

BMDM

BMDM

Rel

ativ

e ex

pres

sion

of C

D20

6 ControlLLC-CMLLC-CM+ O-ATP

BMDM

Rel

ativ

e ex

pres

sion

of A

rg1

ControlLLC-CMLLC-CM+ O-ATP

BMDM

Control

Rel

ativ

e ex

pres

sion

of F

izzl IL4

IL4+ O-ATP

BMDM

Rel

ativ

e ex

pres

sion

of C

D20

6 ControlIL4IL4+ O-ATP

BMDM

I J

Control IL4 IL4+ O-ATP IL4+ A-43 IL4+ A-74

Rel

ativ

e ex

pres

sion

of C

D20

6

MHS

0

50

100

150

200

250*** *

**

Rel

ativ

e ex

pres

sion

of A

rg1

0

1000

2000

3000

4000*** * ***

Rel

ativ

e ex

pres

sion

of F

izzl

Con

trol

IL4

IL4+

A-4

3

IL4+

A-7

4

F4/80

CD

206

4.35% 27.4%

15.7% 9.7%

BMDMControl IL4 IL4+ A-43 IL4+ A-74

BMDM

Qin et al., Sup Fig. 5A

B

0.913% 10% 8.77%8.47%

IFNγ

0.906% 7.48% 8.70%9.63%

TNFα

CD

4+T

cells

α-CD3/CD28Controlα-CD3/CD28

+WT Mϕα-CD3/CD28+P2X7-/- Mϕ

%IF

Nγ

+C

D4+

T c

ells

%TN

Fα+

CD

4+T

cells

Control α-CD3/CD28 α-CD3/CD28+WT Mϕα-CD3/CD28+P2X7-/- Mϕ

A B C

PBS

A-43

Cisplatin

A-43+Cis

A-74+Cis

A-74

Tum

or w

eigh

t (g)

Qin et al., Sup Fig. 6

Volu

me

of tu

mor

(mm

3 ) PBS

A-43Cisplatin

A-43+ Cis

Day

A-74+ CisA-74

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