q cycle ubiquinone (q) pool is critical to h + shuttling to maintain the pmf and drive...
Post on 19-Dec-2015
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Q cycle• Ubiquinone (Q) pool is critical to H+ shuttling
to maintain the pmf and drive phosphorylation to produce ATP
Quinone (Q) is reduced with an electron through cytochrome bc1 to form hydroquinone (QH2) – QH2 is then oxidized by another site on cytochrome bc1 back to Q – this shuttles H+ across the membrane, the H+ goes through ATPase back into the cell, generating ATP
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Growth
• Monod equation – accounts for substrate availability and idea that organism can only eat so fast… (saturation of transporters)
•Michaelis-Menton equation, derived from enzyme activities, also accounts for substrate availability and idea that specific number of enzymes can only work so fast…
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• Specific growth rate, m, (time-1)• Specific metabolic rate, V, (mmol cell-1 h-1)
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Microbial Communities
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‘thiovulum’ mats, Pozzo di Cristale, Frassassi caves
0.5 m
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• Biofilm formation
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Biofilm characteristics:dynamic, complex structures
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Biofilms’ genetic expression different
• When in a biofilm the same organism express different proteins
• Functions become specialized
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• Different cells communicate to coordinate behaviors/genetic expression in a biofilm
• Cells can sense when there is a sufficient density of cells and the conditions to create a biofilm – quorum sensing
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Capabilities of the group
• Biofilms are ore resistant to attack than planktonic expressions of the same organisms (attack = antimicrobial agents, viruses, physicochemical changes)
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