pseudomonas identification
DESCRIPTION
Pseudomonas Identification. Done By : Omniya Tayem Dr: Adbelraouf Elmanama Mohamad Laqan. Structure & Physiology. Pseudomonas. * Gram _ Negative rods. Gram negative rod. Motile with polar flagella *. - PowerPoint PPT PresentationTRANSCRIPT
Pseudomonas IdentificationPseudomonas Identification
Done ByDone By : :
Omniya TayemOmniya Tayem
Dr: Adbelraouf ElmanamaDr: Adbelraouf Elmanama
Mohamad LaqanMohamad Laqan
Structure & PhysiologyStructure & Physiology
* *Gram _ Negative rodsGram _ Negative rods
Gram negative rodGram negative rod
Motile with polar flagellaMotile with polar flagella **
Motility positiveMotility positive
Obligate aerobeObligate aerobe
Oxidase _ positiveOxidase _ positive* *
Do not ferment Do not ferment Carbohydrates Carbohydrates
Resistant to multiple drugsResistant to multiple drugs
Majority of human infectionsMajority of human infections
_ _ P. aeruginosaP. aeruginosa
Common in the environmentCommon in the environment
. .WaterWater
. .AirAir
..SoilSoil
Very simple nutritional requirementsVery simple nutritional requirements
Many organic compound used asMany organic compound used as
C & N sources , but onlyC & N sources , but only
afew carbohydrates byafew carbohydrates by
oxidative metabolismoxidative metabolism
Some strains appear mucoid due toSome strains appear mucoid due to
Polysaccharide CapsulePolysaccharide Capsule
Some produce diffusible pigmentsSome produce diffusible pigments
PyocyaninPyocyanin _ _
nonfluorescent bluish pigmentnonfluorescent bluish pigment
PyoverdinPyoverdin _ _
Fluorescent greenish pigmentFluorescent greenish pigment
Pyorubin & PyomelaninPyorubin & Pyomelanin
Fluorescent PigmentsFluorescent Pigments
Pseudomonas . aeruginosaPseudomonas . aeruginosa
Pseudomonas aeruginosa colonies Pseudomonas aeruginosa colonies on Agaron Agar
Pseudomonas . aeruginosaPseudomonas . aeruginosa
Identification of P. AeruginosaIdentification of P. Aeruginosa
Usually based onUsually based on
colonial morphologycolonial morphology , ,
B- hemolysisB- hemolysis , ,
Oxidase positivityOxidase positivity , ,
The presence of characteristic pigmentsThe presence of characteristic pigments
& &Sweet odorSweet odor
& &Growth at 42cGrowth at 42c
Identification Of PseudomonasIdentification Of Pseudomonas
PigmentsPigments **Pyocyanin (blue _ green )Pyocyanin (blue _ green )
Fluorescein (green _yellow_fluorescent )Fluorescein (green _yellow_fluorescent )
Pyorubin (red _ brown )Pyorubin (red _ brown )
Culures have fruity smellCulures have fruity smell **
Biochemical reactionsBiochemical reactions* *
..Gelatine hydrolysisGelatine hydrolysis
..Starch hydrolysisStarch hydrolysis
..Carbohydrates useCarbohydrates use
Trehalose (O_F medium)Trehalose (O_F medium)
..Flourescent PigmentFlourescent Pigment
P. aeruginosaP. aeruginosa
Gelatin hydrolysis Gelatin hydrolysis +/ - +/ - ( ( variable result variable result ))
Starch hydrolysis Starch hydrolysis + / - + / - ( ( variable result variable result ))
Acid Aerobically from D_ TrehaloseAcid Aerobically from D_ Trehalose
) ) negativenegative ( (
Fluorescent pigment ( Fluorescent pigment ( positive positive ))
Non Fluorescent pigment ( Non Fluorescent pigment ( positive positive ))
P .fluorescensP .fluorescens
Gelatin hydrolysis ( Gelatin hydrolysis ( positive positive ))
Starch hydrolysis ( Starch hydrolysis ( negative negative ))
Acid aerobically from D _ Trehalose Acid aerobically from D _ Trehalose
( (positivepositive))
Fluorescent pigment ( Fluorescent pigment ( negative negative ))
Non fluorescent pigment (Non fluorescent pigment (positive positive ))
P. SaccharophilaP. Saccharophila
Gelatin hydrolysis (Gelatin hydrolysis (PP))
Starch hydrolysis (Starch hydrolysis (PP))
Acid aerobically from D_ Trehalose (Acid aerobically from D_ Trehalose (P P ))
Fluorescent pigment ( Fluorescent pigment ( N N ))
Non fluorescent pigment ( Non fluorescent pigment ( N N ))
P. PutidaP. Putida
Gelatine hydrolysis ( Gelatine hydrolysis ( negative negative ))
Starch hydrolysis (Starch hydrolysis (negative negative ))
Acid aerobically from D_ Trehalose (Acid aerobically from D_ Trehalose (N N ))
Fluorescent pigment ( Fluorescent pigment ( P P ))
Non fluorescent pigment ( Non fluorescent pigment ( P P ))
The EndThe End